Summary
Experiments have been conducted on different methods of storing soils and soil extracts to preserve the mineral-N status unaltered. Neither the addition of chemicals at the rates used nor storage at sub-zero temperatures were found to preserve the mineral-N status of the soils completely unaltered over short periods of time. Furthermore, evidence was found to show that storage at sub-zero temperatures can effect subsequent mineralization of nitrogen during incubation.
The mineral-N content of soil extracts prepared with an acidified K2SO4 reagent was not preserved over short periods by the addition of chemicals, by storing the extracts at sub-zero temperatures or by the addition of copper ions to the extracting solution. The mineral-N content of soil extracts prepared with a neutral KCl reagent could be preserved during storage at subzero temperatures but the addition of copper ions to the extracting reagent was not effective.
Changes in the mineral-N content of both soils and soil extracts during storage were not consistent and in the soils appeared to be related to biological activity and the concentration of mineral N present. In soil extracts changes appeared to be related to the concentration of mineral-N present and to some other factor(s).
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References
Beck, von Th. and Poschenrieder, H., Versuche über die Wirkung von Toluene auf die Microflora von Boden. Plant and Soil18, 346–357 (1963).
Birch, H. F., Further observations on humus decomposition and nitrification. Plant and Soil9, 262–286 (1959).
Bremner, J. M., Rothamsted Exp. Sta. Rept., 59 (1959).
Bremner, J. M., Methods of Soil Analysis. Edited by Black, C. A.et al., Series Agron. No. 9, published by Am. Soc. Agron. Inc., Madison, Wisconsin, U.S.A., Vol.2, 1199 (1965).
Bremner, J. M., Methods of Soil Analysis. Edited by Black, C. A.et al., Series Agron. No. 9, published by Am. Soc. Agron. Inc., Madison, Wisconsin, U.S.A., Vol.2, 1195–1198 (1965).
Bremner, J. M., Methods of Soil Analysis. Edited by Black, C. A.et al., Series Agron. No. 9, published by Am. Soc. Agron. Inc., Madison, Wisconsin, U.S.A., Vol.2, 1338–1340 (1965).
Bremner, J. M., Methods of Soil Analysis, Edited by Black, C. A.et al., Ser. Agron. No. 9, published by Am. Soc. Agron. Inc., Madison Wisconsin, U.S.A., Vol.2, 1187–1191 and 1205 (1965).
Bremner, J. M., Methods of Soil Analysis. Edited by Black C. A.et al., Series Agron. No. 9, published by Am. Soc. Agron. Inc., Madison, Wisconsin, U.S.A. Vol.2, 1341 (1965).
Bremner, J. M. and Keeney, D. R., Steam distillation methods for determination of ammonium, nitrate and nitrite. Anal. Chim. Acta.32, 485–495 (1965).
Bremner, J. M. and Shaw, K., Determination of ammonia and nitrate in soil. J. Agr. Sci.46, 320–328 (1955).
Buddin, W., Partial sterilisation of soil by volatile and non-volatile antiseptics. J. Agr. Sci.6, 417–451 (1914).
Clement, C. R. and Williams, T. E., An incubation technique for assessing the nitrogen status of soils newly ploughed from leys. J. Soil Sci.13, 82–91 (1962).
D'Hoore, J. L., Soil Map of Africa. Explanatory Monograph, Project No. 11. Comm. Tech. Co-op. in Africa, Lagos, 1964, p. 103–104. (1964).
Gasser, J. K. R., Use of deep-freezing in the preservation and preparation of fresh soil samples. Nature181, 1334–1335 (1958).
Grossbard, E. and Hall, D. M., An investigation into possible changes in the microbial population of soils stored at −15°C. Plant and Soil.21, 317–332 (1964).
Jackson, M. L., soil Chemical Analysis. Published by Constable & Co. Ltd., London, p. 198 (1958).
Lewis, D. G., Determination of inorganic nitrogen in soil. J. Sci. Food Agr.12, 735–742 (1961).
Mack, A. R., Biological activity and mineralization of nitrogen in three soils as induced by freezing and drying. Canad. J. Soil Sci.43, 316–324 (1963).
Piper, C. S., Soil and Plant Analysis. Published by the Univ. of Adelaide, 1st. edition, 203, (1950).
Robinson, J. B. D., Experiments to determine the influence of pre-drying soil samples on their behaviour during incubation. Ann. Rept. Coffee Research Services, Kenya1965/57, 65–68 (1957).
Russell, E. J. and Hutchinson, H. B., The effect of partial sterilization of soil on the production of plant food. II. The limitation of bacterial numbers in normal soils and its consequences. J. Agric. Sci.5, 152–221 (1912–1913).
Shaw, K., Personal communication, (1965).
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Part of a thesis submitted for the degree of Ph. D in the University of East Africa.
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Robinson, J.B.D. The preservation unaltered, of mineral nitrogen in tropical soils and soil extracts. Plant Soil 27, 53–80 (1967). https://doi.org/10.1007/BF01373978
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DOI: https://doi.org/10.1007/BF01373978