Abstract
Studies were conducted to determine the effects of a simulated Martian environment on the survival of terrestrial microorganisms. Mariner IV data were utilized to establish the Martian model. Day/night cycling, temperature, humidity, pressure, atmospheric constituents and solar irradiation were controlled in a 3600 ft3 simulation chamber at the Boeing Kent Space Simulation Laboratory.
Microorganisms used in this study included suspensions of:Bacillus subtilis var.niger spores, a psychrophilic sporeformer, and the organisms contained in a soil emulsion prepared from several soils. Aliquots of each suspension were placed on separate sterile stainless steel planchets, air dried, and positioned in 4 layers in sterile limonite. One-half of the samples placed on the surface received the total solar spectrum (0.44 earth solar constants); the remainder of the surface samples received the above treatment minus the ultraviolet (UV) portion of the spectrum. Subsurface samples were placed in limonite at depths of 0.5, 1.5 and 3 in.
Survival data were obtained for chamber exposure periods of 2, 4, and 8 days. These data indicate that: (1) organisms exposed to the total solar spectrum did not survive, (2) a time dependent, 1 to 3 log, reduction in numbers occurred in the surface samples that received the solar spectrum minus UV, and (3) subsurface survival varied with depth and type or organism.
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References
Angelotti, R.: 1968 ‘Protective Mechanisms Affecting Dry Heat Sterilization’,COSPAR Technique Manual Series 4, 59–74.
COSPAR Information Bulletin No. 20, November 1964, 25–26.
Fox, K. and Pflug, I. J.: 1968, ‘Effect of Temperature and Gas Velocity on the Dry Heat Destruction Rate of Bacterial Spores’,Appl. Microbiol. 16, 343–348.
Hagen, C. A. and Jones, R.: 1963, ‘Life in Extraterrestrial Environments’, Quarterly Status Report, Sept–Nov. NASr 22:IITRI Proj. C194.
Hagen, C. A., Hawrylewicz E. J., and Ehrlich, R.: 1967, ‘Survival of Microorganisms in a Simulated Martian Environment, II. Moisture and Oxygen Requirements for Germination ofBacillus cereus andBacillus subtilis var.niger Spores,Appl. Microbiol. 15, 285–291.
Hall, L. B.: 1968, ‘The Importance of Sterilization Techniques in Space Exploration’,COSPAR Technique Manual Series 4, 3–18.
Hawrylewicz, E. J., Hagen, C. A., and Ehrlich, R.: 1965, ‘Response of Microorganisms to a Simulated Martian Environment’,Life Sci. Space Res. III, 64–73.
Kliore, A., Cain, D. L., Levy, G. S., Eshleman, V. R., Fjeldbo G., and Drake, F. D.: 1965, ‘Occulation Experiment: Results of the First Direct Measurement of Mar's Atmosphere and Ionosphere’,Science 149, 1243–1248.
Murrell, W. G. and Scott, W. J.: 1966, ‘The Heat Resistance of Bacterial Spores at Various Water Activities’,J. Gen. Microbiol. 43 411–425.
Olson, R. L., Green, R. H., Gustan, E. A., and Pilgrim, A. J.: 1967, ‘Microbial Survival After Simulated Meteoroid Impact’,Develop. Ind. Microbiol. 8, 235–245.
Roberts, T. L., 1963, ‘Studies with a Simulated Martian Environment’,J. Astronaut. Sci. X, 65–74.
Silverman, G. J., Davis, N. S., and Keller, W. H.: 1964, ‘Exposure of Microorganisms to Simulated Extraterrestrial Space Ecology’,Life Sci. Space Res. II, 372–384.
Silverman, G. J., Davis, N. S., and Beecher, N.: 1967, ‘Resistivity of Spores to Ultraviolet and Gamma Radiation while Exposed to Ultrahigh Vacuum or at Atmospheric Pressure’,Appl. Microbiol. 15, 510–515.
Young, R. S., Deal, R. H., and Whitfield O. 1968, ‘The Response of Spore Forming vs. Non Spore Forming Bacteria to Diurnal Freezing and Thawing’,Space Life Sci. 1, 113–117.
Zhukova, A. I. and Kondratyev,: 1964, ‘On Artificial Martian Conditions Reproduced for Microbiological Research’,Life Sci. Space Res. III, 120–127.
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Green, R.H., Taylor, D.M., Gustan, E.A. et al. Survival of microorganisms in a simulated Martian environment. Space Life Sciences 3, 12–24 (1971). https://doi.org/10.1007/BF00924210
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DOI: https://doi.org/10.1007/BF00924210