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The subunit proportions and kinetic properties of 6-phosphofructo-1-kinase isozymes from rat heart atria and ventricle progressively change during aging

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Abstract

Relative to 2–3 month rats, total 6-phosphofructo-1-kinase (PFK) activity in heart atria from 12 month rats declined 31%; but, by 24 months it was decreased by only 13%. PFK activities from 12 and 24 month ventricles relative to the 2–3 month rat were decreased by 40% and 30%, respectively. This change in PFK activity in each heart region was associated with alterations of subunit composition. In heart atria from 12 and 24 month rats when compared to 3 month rats, the levels of L-type subunit were not significantly different; but the levels of the M-type subunit were decreased by 43% and 38%, respectively. With respect to levels in 2–3 month atria, the C-type subunit in 12 month atria decreased by 27%; and at 24 months it increased by 31%. Making the same comparison for the heart ventricle at 12 and 24 months, L-type subunit decreased by 30% and 24% respectively; M-type subunit decreased by approximately 47%; and the C-type subunit increased 1.9 and 4.7 fold, respectively. These age-related changes of subunit composition in atrial and ventricular PFK isozyme pools led to changes in their kinetic and regulatory properties suggesting that the aged rat could exhibit a diminished capacity to produce ATP from glucose.

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Abbreviations

PFK:

6-Phosphofructo-1-Kinase

PAGE:

Polyacrylamide Gel Electrophoresis

SDS:

Sodium Dodecyl Sulfate

fructose-2,6-P2 :

D-fructose 2,6-bisphosphate

fructose-6-P:

D-fructose 6-phosphate

References

  1. Sandi DR: Metabolic changes and their significance in aging. Handbook of the Biology or Aging, Finch, CE & Hayflick L (eds.), first edition, Van Nostrand-Reinholt Co., NY, 1977, pp 73–98

    Google Scholar 

  2. Masoro EJ: Metabolism. Handbook of the Biology of Aging, Finch, CE & Schneider EL (eds.), second edition, Van Nostrand-Reinholt Co., NY, 1985, p 540

    Google Scholar 

  3. Wollenberger A, Jehl J: Influence of age on rate of respiration of sliced cardiac muscle. Am J Physiol 170: 126–130, 1952

    PubMed  CAS  Google Scholar 

  4. Barrows CH, Roeder LM, Falzone JA: Effect of age on activities of enzymes and the concentration of nucleic acids in the tissues of wild female rats. J Gerontol 17: 144–147, 1962

    PubMed  CAS  Google Scholar 

  5. Chen JC, Warsaw JB, Sandi DR: Regulation of mitochondrial respiration in senescence. J Cell Physiol 80: 141–148, 1972

    Article  PubMed  CAS  Google Scholar 

  6. Randle PJ, Tubbs PK: Carbohydrate and fatty acid metabolism. Handbook of Physiology, The Cardiovascular System, Berne RM, Sperelakis N (eds.), vol 1. American Physiological Society, Bethesda, MD, 1978, pp 805–844

    Google Scholar 

  7. Dunaway GA: A review of animal phosphofructokinase isozymes with an emphasis on their physiological role, Mol Cell Biochem 52: 75–91, 1983

    Article  PubMed  CAS  Google Scholar 

  8. Dunaway GA, Kasten TP: Physiological relevance of the changing subunit composition and regulatory properties of the 6-phosphofructo-1-kinase isozyme pools during heart and muscle development. Mole Cell Biochem 87: 71–77, 1989

    Article  CAS  Google Scholar 

  9. Dunaway GA, Kasten TP: Characterization of the rat heart 6-phosphofructo-1-kinase isozymes. J Mol Cell Cardiol 17: 947–957, 1985

    PubMed  CAS  Google Scholar 

  10. Dunaway GA, Kasten TP, Naqui D: Insulin-mediated regulation of heart atrial and ventricular 6-phosphofructo-1-kinase. J Biol Chem 261: 7831–7833, 1986

    PubMed  CAS  Google Scholar 

  11. Dunaway GA, Kasten TP: Nature of the subunits of the 6-phosphofructo-1-kinase from rat tissues. Biochem J 242: 667–671, 1987

    PubMed  CAS  Google Scholar 

  12. Dunaway GA, Kasten TP, Kolm P: Alteration of 6-phosphofructo-1-kinase isozyme pools during heart development and aging. J Biol Chem 261: 17170–17173, 1986

    PubMed  CAS  Google Scholar 

  13. Dunaway GA, Kasten TP, Nickols GA, Chesky J: Regulation of skeletal muscle 6-phosphofructo-1-kinase during aging and development. Mech Ageing Dev 36: 13–23, 1986

    Article  PubMed  CAS  Google Scholar 

  14. Olefsky JM, Reaven GM: Effects of age and obesity on insulin binding to isolated adipocytes. Endocrinology 96: 1486–1498, 1975

    Article  PubMed  CAS  Google Scholar 

  15. Gold G, Karoly K, Freeman C, Adelman RC: A possible role for insulin in the altered capability for hepatic enzyme adaption during aging. Biochem Biophys Res Commun 73: 1003–1010, 1976

    Article  PubMed  CAS  Google Scholar 

  16. Bracho-Romero E, Reaven GM: Effect of age and weight on plasma glucose and insulin responses in rat. J Am Geriatr Soc 25: 299–302, 1977

    PubMed  CAS  Google Scholar 

  17. Sartin J, Chaudhuri M, Obenrader M, Adelman RC: The role of hormones in changing adaptive mechanisms during aging. Fed Proc 39: 3163–3167, 1980

    PubMed  CAS  Google Scholar 

  18. Bass A, Gutmann E, Hanzlikova V: Biochemical and histochemical changes in energy supply-enzyme pattern of muscles of rat during old age. Gerontologia 21: 31–45, 1975

    Article  PubMed  CAS  Google Scholar 

  19. Iwangoff P, Reichlmeir K, Enz A, Meier-Ruge W: Neurochemical findings in physiological ageing of the brain. Interdispl Topics Geront 15: 13–33, 1979

    CAS  Google Scholar 

  20. Dunaway GA, Kasten TP: Physiological implications of the alteration of 6-phosphofructo-1-kinase isozyme pools during brain aging and development. Brain Res 456: 310–316, 1988

    Article  PubMed  CAS  Google Scholar 

  21. Fukuda H, Iritani N: Effect of aging on changes in substrate and effector levels of rat liver glycolytic and lipogenic enzymes during induction. Biochim Biophys Acta 795: 79–84, 1984

    PubMed  CAS  Google Scholar 

  22. Hron WT, Menahan LA: Age-related changes in activities of hepatic phosphofructokinase, pyruvate kinase, and pyruvate dehydrogenase, in liver and adipose tissue of the swiss albino mouse. Enzyme 30: 83–88, 1983

    PubMed  CAS  Google Scholar 

  23. Vitorica J, Andres A, Satrustegui J, Machado A: Age-related quantitative changes in enzyme activities of rat brain. Neurochem Res 6: 127–136, 1981

    Article  PubMed  CAS  Google Scholar 

  24. Vitorica J, Cano J, Satrustegui J, Machado A: Comparison between development and senescent changes in enzyme activities linked to energy metabolism in rat heart. Mech Age Dev 16: 105–116, 1981

    Article  CAS  Google Scholar 

  25. Dunaway GA, Kasten TP, Crabtree S, Mhaskar Y: Age-related changes in the subunit composition and regulation of hepatic 6-phosphofructo-1-kinase. Biochem J 266: 823–827, 1990

    PubMed  CAS  Google Scholar 

  26. Richardson A, Cheung HT: The relationship between age-related changes in gene expression, protein turnover, and responsiveness of an organism to stimuli. Life Sci 31: 605–613, 1982

    Article  PubMed  CAS  Google Scholar 

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  1. Department of Pharmacology, Southern Illinois University School of Medicine, P.O. Box 19230, 62794-9230, Springfield, IL, USA

    Yashanad Mhaskar & George A. Dunaway

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  1. Yashanad Mhaskar
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  2. George A. Dunaway
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Mhaskar, Y., Dunaway, G.A. The subunit proportions and kinetic properties of 6-phosphofructo-1-kinase isozymes from rat heart atria and ventricle progressively change during aging. Mol Cell Biochem 107, 39–45 (1991). https://doi.org/10.1007/BF02424574

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  • DOI: https://doi.org/10.1007/BF02424574

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