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Regulation of Bacterial Communities Through Antimicrobial Activity by the Coral Holobiont

  • Host Microbe Interactions
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Abstract

Interactions between corals and associated bacteria and amongst these bacterial groups are likely to play a key role in coral health. However, the complexity of these interactions is poorly understood. We investigated the functional role of specific coral-associated bacteria in maintaining microbial communities on the coral Acropora millepora (Ehrenberg 1834) and the ability of coral mucus to support or inhibit bacterial growth. Culture-independent techniques were used to assess bacterial community structures whilst bacterial culture was employed to assess intra- and inter-specific antimicrobial activities of bacteria. Members of Pseudoalteromonas and ribotypes closely related to Vibrio coralliilyticus displayed potent antimicrobial activity against a range of other cultured isolates and grew readily on detached coral mucus. Although such bacterial ribotypes would be expected to have a competitive advantage, they were rare or absent on intact and healthy coral colonies growing in situ (analysed using denaturing gradient gel electrophoresis and 16S rRNA gene sequencing). The most abundant bacterial ribotypes found on healthy corals were Gammaproteobacteria, previously defined as type A coral associates. Our results indicate that this group of bacteria and specific members of the Alphaproteobacteria described here as ‘type B associates’ may be important functional groups for coral health. We suggest that bacterial communities on coral are kept in check by a combination of host-derived and microbial interactions and that the type A associates in particular may play a key role in maintaining stability of microbial communities on healthy coral colonies.

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Acknowledgements

This project was supported in part by grants from the PADI Foundation, the Australian Coral Reef Society and the Winifred Violet Scott Foundation.

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Authors and Affiliations

  1. Centre for Marine Science, The University of Queensland, St Lucia, Brisbane, Queensland, 4072, Australia

    E. Charlotte E. Kvennefors, Eugenia Sampayo, Caroline Kerr, Genyess Vieira & Andrew C. Barnes

  2. School of Biological Sciences, The University of Queensland, St Lucia, Brisbane, Queensland, 4072, Australia

    George Roff & Andrew C. Barnes

  3. ARC Centre of Excellence for Coral Reef Studies, The University of Queensland, St Lucia, Brisbane, Queensland, 4072, Australia

    E. Charlotte E. Kvennefors & George Roff

Authors
  1. E. Charlotte E. Kvennefors
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  2. Eugenia Sampayo
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  4. Genyess Vieira
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  5. George Roff
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  6. Andrew C. Barnes
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Correspondence to E. Charlotte E. Kvennefors.

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Kvennefors, E.C.E., Sampayo, E., Kerr, C. et al. Regulation of Bacterial Communities Through Antimicrobial Activity by the Coral Holobiont. Microb Ecol 63, 605–618 (2012). https://doi.org/10.1007/s00248-011-9946-0

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  • DOI: https://doi.org/10.1007/s00248-011-9946-0

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