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Winter–Summer Succession of Unicellular Eukaryotes in a Meso-eutrophic Coastal System

  • Microbiology of Aquatic Systems
  • Published:
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Abstract

The objective of this study was to explore the succession of planktonic unicellular eukaryotes by means of 18S rRNA gene tag pyrosequencing in the eastern English Channel (EEC) during the winter to summer transition. The 59 most representative (>0.1 %, representing altogether 95 % of total reads), unique operational taxonomic units (OTUs) from all samples belonged to 18 known high-level taxonomic groups and 1 unaffiliated clade. The five most abundant OTUs (69.2 % of total reads) belonged to Dinophyceae, Cercozoa, Haptophyceae, marine alveolate group I, and Fungi. Cluster and network analysis between samples distinguished the winter, the pre-bloom, the Phaeocystis globosa bloom and the post-bloom early summer conditions. The OTUs-based network revealed that P. globosa showed a relatively low number of connections—most of them negative—with all other OTUs. Fungi were linked to all major taxonomic groups, except Dinophyceae. Cercozoa mostly co-occurred with the Fungi, the Bacillariophyceae and several of the miscellaneous OTUs. This study provided a more detailed exploration into the planktonic succession pattern of the EEC due to its increased depth of taxonomic sampling over previous efforts based on classical monitoring observations. Data analysis implied that the food web concept in a coastal system based on predator–prey (e.g. grazer–phytoplankton) relationships is just a part of the ecological picture; and those organisms exploiting a variety of strategies, such as saprotrophy and parasitism, are persistent and abundant members of the community.

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Acknowledgements

This study was supported by the INSU/CNRS/EC2CO-IMMERSE (EC2CO_2011), the ‘Nord-Pas de Calais’ Region and FRB-DEMO (FRB_2013), the ANR-ROME (ANR 12 BSV7 0019 02), the BQR-ULCO 2012 projects and the SOMLIT network. We are grateful to Eric Lecuyer and JD Grattepanche for the sampling assistance. www.englisheditor.webs.com is acknowledged for the paper’s English proofing. Finally, we are grateful to four anonymous reviewers for their constructive comments, which improved our work.

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  1. Laboratoire d’Océanologie et Géosciences (LOG), UMR CNRS 8187, Université du Littoral Côte d’Opale (ULCO), 32 av. Foch, 62930, Wimereux, France

    Urania Christaki, Savvas Genitsaris, Clément Georges & Sébastien Monchy

  2. Department of Ichthyology and Aquatic Environment, University of Thessaly, 384 46, Nea Ionia, Greece

    Konstantinos A. Kormas

  3. Laboratoire Microorganismes: Génome et Environnement (LMGE), UMR CNRS 6023, Clermont Université Blaise Pascal, BP 80026, 63171, Aubière Cedex, France

    Télesphore Sime-Ngando

  4. Center for Infection and Immunity of Lille (CIIL), Institut Pasteur of Lille, Inserm U1019, CNRS UMR 8204, Biology and Diversity of Emerging Eukaryotic Pathogens, EA4547, Université Lille Nord de France, Lille, France

    Eric Viscogliosi

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  1. Urania Christaki
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Christaki, U., Kormas, K.A., Genitsaris, S. et al. Winter–Summer Succession of Unicellular Eukaryotes in a Meso-eutrophic Coastal System. Microb Ecol 67, 13–23 (2014). https://doi.org/10.1007/s00248-013-0290-4

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