Abstract
Larvae of sea bass (Dicentrarchus labrax) and sea bream (Sparus aurata) were transferred to normal or glucose-enriched sea water immediately after mouth opening to assess their ability to absorb and assimilate glucose at the beginning of the larval period. Assimilation was monitored by histological and cytochemical analysis of the liver. The results showed that (1) the larvae of both species regularly ingested water, (2) glucose absorption resulted in glycogen accumulation in the hepatocytes (this was more marked in sea bass than in sea bream), and (3) glucose delayed the pathological effects of fasting. Consideration of metabolic derivatives indicates that hepatic glycogen probably arises from neoglucogenesis.
Similar content being viewed by others
References
Baker N (1977) Measurement of glucose recycling and liver glycogen synthesis in mice using labeled substrates. Fedn Proc Fedn Am Socs exp Biol 36:253–258
Bashan N, Gross V, Moses S, Gutman A (1979) Rat liver glycogen metabolism in the perinatal period. Biochim biophys Acta 587: p 145
Beaumont A (1958) Etude expérimentale de l'apparition du glycogène hépatique chez les larves d'amphibiens anoures. Thèse. Faculté des Sciences de Paris
Bedard D, Lalancette LM (1989) Etude du développement larvaire de l'Eperlan, Osmerus mordax, en eau pure et dans deux milieux de matière organique, le glucose et l'acétate. Aquaculture, Amsterdam 80:351–362
Cowey CB, Adron JW, Brown DA, Shanks AM (1975) Studies on the nutrition of marine flatfish. The metabolism of glucose by plaice (Pleuronectes platessa) and the effect of dietary energy source on protein utilization in plaice. Br J Nutr 33:219–231
Cowey CB, Sargent JR (1979) Nutrition. In: Hoar WS, Randall DI, Brett JR (eds) Fish physiology. VIII. Bioenergetics and growth, Academic Press, New York, pp 1–69
Daimon T, David H, Zglinicki VT, Marx I (1982) Correlated ultrastructural and morphometric studies on the liver during prenatal development of rats. Expl Path 121:237–250
Devos P, Hers HG (1973) Glycogen metabolism in the liver of the fetal rat. Biochem J 140:331–340
Diaz JP, Connes R (1991) Development of the liver of the sea bass, Dicentrarchus labrax L. (teleost, fish). II. Hepatocyte differentiation. Biol Struct Morphogen 3:57–65
Diaz JP, Guyot E, Chatain B, Vigier S Connes R (1992) Altérations hépatiques d'origine nutritionnelle chez les larves du loup (Dicentrarchus labrax) et de la daurade (Sparus aurata) en élevage. In: Vivarès CP (ed) Fifth International Colloquiun on Pathology in Marine Aquaculture. [Abstracts] BIOCIM, Montpellier, p 81
Fänge R, Grove D (1979) Digestion. In: Hoar WS, Randall DJ, Brett JR (eds) Fish physiology. VIII. Bioenergetics and growth, Academic Press, New York, pp 162–260
Fauconneau B, Kaushik SJ, Blanc JM (1989) Uptake and metabolization of dissolved compounds in rainbow trout (Salmo gairdneri R.) fry. Comp Biochem Physiol 93 A:839–843
Favard P, Jost A (1966) Différenciation et charge en glycogène de l'hépatocyte du foetus de rat normal ou décapité (étude au microscope électronique). Archs Anat microsc Morph exp 55: 603–632
Fiala-Médioni A (1988) Matière organique dissoute et production secondaire (adultes). Océanis, Paris 14:399–407
Guyot E, Connes R, Diaz JP (1993) Résorption des réserves vitellines et passage de l'endotrophie à l'exotrophie chez la larve de Daurade (Spatus aurata) nourrie et à jeun. In: Barnabé G, Kestemont P (eds) Production, environment and quality. Bordeaux Aquaculture 92. European Aquaculture Society, Ghent, Belgium, pp 213–226 (Speo Publs eur Aquacult Soc No 18)
Le Gal Y (ed) (1988) La matière organique dans l'environnement marin. Biochimie marine. Masson et Cie, Paris, pp 1–8
Martoja R, Martoja M (1967) Inltiation aux techniques de l'histologie animale, Masson et Cie, Paris
Mosconi-Bac N (1987) Pepatic disturbances induced by an artificial feed in the sea bass (Dicentrarchus labrax) during the first year of life. Aquaculture, Amsterdam 67:93–99
Newgard CB, Moore SV, Foster DW, McGarry JD (1984) Efficient hepatic glycogen synthesis in refeeding rats requires continued carbon flow through the gluconeogenic pathway. J biol Chem 259:6958–6963
Pavillon JF (1988) Matière organique dissoute et production secondaire (larves). Océanis, Paris 14:389–397
Pavillon JF, Vu TT (1981) Un aspect de l'absorption des substances organiques dissoutes chez les organismes marins et d'eaux saumâtres: données autoradiographiques aux premiers stades du développement chez Artemia species (ex salina) et Dicentrarchus labrax. Océanis, Paris 7:705–718
Radziuk J (1990) The liver and glycogen metabolism. J parent ent Nutr 15:77–81
Randall GCB, L'Ecuyer C (1976) Tissue glycogen and blood glucose and fructose in the pig fetus during the second half of gestation. Biol Neonate 28:74–82
Sandström B, Westman J (1971) Ultrastructure of the developing chicken, liver before hatching. Z Zellforsch 117:516–525
Segner H, Witt U (1990) Weaning experiments with turbot (Scophtalmus maximus): electron microscopic study of liver. Mar Biol 105:353–361
Seligman AM, Wasserkrug HL, Hanker JS (1966) A new staining method (OTO) for enhancing contrast of lipid-containing membranes and droplets in osmium tetroxide-fixed tissue with osmiophilic thiocarbohydrazide (TCH). J Cell Biol 30:424–432
Singh J (1964) A modification of the Masson-Hamperl method for staining of argentaffin cells. Anat Anz 115:81–82
Sire MF, Vernier JM (1980) Lipid staining on semithin sections with Sudan black B or Nile blue sulphate. Application to intestinal fat absorption. Acta Histochem Cytochem 13:193–201
Storch V, Stählin W, Juraio JV (1983) Effect of different diets on the ultrastructure of Chanos chanos fry (Chanidae: Teleostei): an electron microscopic and morphometric analysis. Mar Biol 74: 101–104
Terner C (1968) Studies of metabolism in embryonic development. I. The oxidative metabolism of unfertilized and embryonated eggs in the rainbow trout. Comp Biochem Physiol 24:941–950
Vernier JM, Sire MF (1976) Evolution of the glycogen content and of glucose-6-phosphatase activity in the liver of Salmo gairdneri during development. Tissue Cell 8:531–546
Verreth J, Storch V, Segner H (1987) A comparative study on the nutritional quality of decapsulated Artemia cysts, micro-encapsulated egg diets and enriched dry feeds for Clarias gariepinus (Burchell) larvae. Aquaculture, Amsterdam 63:269–282
Author information
Authors and Affiliations
Additional information
Communicatd by J. M. Pérès, Marseille
Rights and permissions
About this article
Cite this article
Diaz, J.P., mani-Ponset, L., Guyot, E. et al. Assimilation of dissolved glucose from sea water by the sea bass Dicentrarchus labrax and the sea bream Sparus aurata . Marine Biology 120, 181–186 (1994). https://doi.org/10.1007/BF00349677
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00349677