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Vitis vinifera canes, a source of stilbenoids against Spodoptera littoralis larvae

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Abstract

Stilbenes are constitutively accumulated in grape canes at a high concentration of about 10 g kg−1 DW. Some of these compounds have antimicrobial activity and are involved in grapevine defence in leaves and fruits, acting as phytoalexins. In this work, we studied their insecticidal effects against Spodoptera littoralis larvae by using an extract from grapevine canes containing 27% (w/w) of total stilbenes and the major ones isolated from this extract (E-piceatannol, E-resveratrol, isohopeaphenol, E-ε-viniferin and E-vitisin B). The stilbenes isolated from the grapevine extract did not show any significant antifeedant effect or acute toxicity, but they did cause chronic mortality to the larvae population of an important polyphagous pest, S. littoralis. The tetramer, E-vitisin B, appeared to be the most effective compound with LC50 and LC90 values of 134 and 298 µg cm−2, respectively. Significant synergic relationships were found between some stilbenes, resulting in a significant increase in the insecticidal efficacy of the mixture of these substances. Taken together, these results suggest that grapevine canes are a good source of active substances for the development of botanical insecticides.

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Acknowledgements

The authors would like to thank the Technology Agency of the Czech Republic for their financial support of the botanical pesticide and basic substance research. Financial support for this work was provided by the Technology Agency of the CR (Project No. TA04020103).

Funding

The authors also wish to thank the Conseil Regional d’Aquitaine for their financial support in this research. The work was supported by the French National Research Agency (ANR LabCom program, Stilbene Innovation project, ANR-14-LAB5-0005-01), the Bordeaux Metabolome Facility and MetaboHUB (ANR-11-INBS-0010 project).

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Authors and Affiliations

  1. Crop Research Institute, Research Team – Biologically Active Substances in Crop Protection, Drnovska 507, 161 06, Prague 6 Ruzyne, Czech Republic

    Roman Pavela

  2. Univ. Bordeaux, Faculté des Sciences Pharmaceutiques, ISVV, EA 4577 Oenologie, Molécules d’Intérêt Biologique (GESVAB), 33882, Villenave d’Ornon, France

    Pierre Waffo-Teguo, Benoît Biais, Tristan Richard & Jean-Michel Mérillon

  3. ISVV, USC 1366 Oenologie, INRA, Villenave d’Ornon, France

    Pierre Waffo-Teguo, Benoît Biais, Tristan Richard & Jean-Michel Mérillon

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  1. Roman Pavela
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  2. Pierre Waffo-Teguo
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  4. Tristan Richard
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  5. Jean-Michel Mérillon
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Correspondence to Jean-Michel Mérillon.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Communicated by M.B. Isman.

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Pavela, R., Waffo-Teguo, P., Biais, B. et al. Vitis vinifera canes, a source of stilbenoids against Spodoptera littoralis larvae. J Pest Sci 90, 961–970 (2017). https://doi.org/10.1007/s10340-017-0836-1

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  • DOI: https://doi.org/10.1007/s10340-017-0836-1

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