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Identification of odor volatile compounds and deodorization of Paphia undulata enzymatic hydrolysate

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Abstract

Unfavorable fishy odour is an inevitable problem in aquatic products. In the present study, headspace solid-phase microextraction gas chromatography mass spectrometry (HS-SPME-GC-MS) analysis of volatiles from untreated samples and three deodorized samples (under the optimal conditions) of Paphia undulata enzymatic hydrolysate revealed that the compounds contributing to the distinctive odor were 1-octen-3-ol, n-hexanal, n-heptanal, 2,4-heptadienal, and 2,4-decadienal, whereas n-pentanal, n-octanal, n-octanol, benzaldehyde, 2-ethylfuran and 2-pentylfuran were the main contributors to the aromatic flavor. The deodorizing effects of activated carbon (AC) adsorption, yeast extract (YE) masking and tea polyphenol (TP) treatment on a P. undulata enzymatic hydrolysate were investigated using orthogonal experiments with sensory evaluation as the index. The following optimized deodorization conditions were obtained: AC adsorption (35 mg mL−1, 80°C, 40 min), YE masking (7 mg mL−1, 45°C, 30 min) and TP treatment (0.4 mg mL−1, 40°C, 50 min). AC adsorption effectively removed off-flavor volatile aldehydes and ketones. YE masking modified the odor profile by increasing the relative contents of aromatic compounds and decreasing the relative contents of aldehydes and ketones. The TP treatment was not effective in reducing the odor score, but it significantly reduced the relative content of aldehydes while increasing that of alkanes. It is also notable that TP effectively suppressed trimethylamine (TMA) formation in a P. undulate hydrolysate solution for a period of 72 h.

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Authors and Affiliations

  1. Key Laboratory of Tropical Marine Bio-resource and Ecology/Guangdong Key Laboratory of Marine Meteria Medica, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, P. R. China

    Deke Chen, Hua Chen, Bingna Cai, Peng Wan, Xiaolian Zhu, Huili Sun & Jianyu Pan

  2. University of Chinese Academy of Sciences, Beijing, 100049, P. R. China

    Deke Chen & Xiaolian Zhu

  3. Department of Chemistry and Chemical Engineering, Foshan University, Foshan, 528000, P. R. China

    Xin Chen

  4. Rutgers University, 98 Brett Road, Piscataway, New Jersey, 08854, USA

    Han Sun

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  1. Deke Chen
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  2. Xin Chen
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  3. Hua Chen
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  4. Bingna Cai
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  5. Peng Wan
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  7. Han Sun
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  8. Huili Sun
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  9. Jianyu Pan
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Correspondence to Jianyu Pan.

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Chen, D., Chen, X., Chen, H. et al. Identification of odor volatile compounds and deodorization of Paphia undulata enzymatic hydrolysate. J. Ocean Univ. China 15, 1101–1110 (2016). https://doi.org/10.1007/s11802-016-3166-7

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  • DOI: https://doi.org/10.1007/s11802-016-3166-7

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