Abstract
Nanoparticles with an inner core of calcium phosphate, followed by layers of DNA and calcium phosphate and an outer layer of poly(ethyleneimine) (PEI) were prepared, characterized, and tested on different cell lines (HeLa, T24, and NIH3T3). A considerable increase in transfection efficiency was found for such nanoparticles, compared to the commercial reagent Polyfect® (a cationic dendrimer). The DNA is incorporated into the nanoparticles and protected from the attack by enzymes (nucleases) inside the cytoplasm of cells. The outer layer of PEI leads to electrosteric colloidal stabilization and gives a positive charge to the nanoparticle, which is helpful for the penetration through the negatively charged cell membrane.
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Acknowledgement
We thank Mr. Georges von Degenfeld and Ms. Katrin Nickel (Bayer Schering Pharma AG, Wuppertal, Germany) for the kind donation of the plasmid pcDNA3.1(+)-firefly-luciferase. Furthermore we thank the Deutsche Forschungsgemeinschaft (DFG) for funding to M.E. and R.H (grants Ep 22/23-1 and He 1398/19-1).
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Sokolova, V., Neumann, S., Kovtun, A. et al. An outer shell of positively charged poly(ethyleneimine) strongly increases the transfection efficiency of calcium phosphate/DNA nanoparticles. J Mater Sci 45, 4952–4957 (2010). https://doi.org/10.1007/s10853-009-4159-3
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DOI: https://doi.org/10.1007/s10853-009-4159-3