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Preparation, characterization, and in vitro evaluation of nanostructured chitosan/apatite and chitosan/Si-doped apatite composites

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Abstract

Chitosan/apatite (CHI/Ap) composites are attracting great attention as biomaterials for bone repair and regeneration procedures. The reason is their unique set of properties: bioactivity and osteoconductivity provided by Ap and resorbability supplied by CHI among others. Thus, in this study, CHI/Ap and CHI/Si-doped Ap composites were prepared and characterized. Particle size, surface area, in vitro physiological stability, enzymatic biodegradation, and bioactivity were evaluated. Unimodal particle size distribution was obtained for composites with high CHI/Ap ratios while bimodal distribution was present in composites with low CHI/Ap ratio. Physiological stability decreased with Si doping and with the CHI content. Acetylation degree and molecular weight of CHI did not affect in vitro stability. Rate of enzymatic degradation increased with the CHI content in composites. Si-doped Ap composites also showed increased degradation with respect to non-doped ones. The bioactivity of the composites was evidenced by the deposition on their surface of a calcium phosphate layer with Ap morphology after immersion in simulated body fluid. Both, biodegradation and bioactivity were dependent on the molecular weight of the polymeric CHI matrix. These results suggest that the CHI/Ap composites obtained are promising materials for bone regeneration applications.

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Acknowledgements

We thank the Royal Society UK for funding the International Joint Project “Development of CHI/calcium phosphate-based bone implants and scaffolds.” The participation of R.G. Carrodeguas was funded by Projects CICYT-MAT2010-17753 and CSIC JAEDoc 09-00093.

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Authors and Affiliations

  1. Centro de Biomateriales, Universidad de La Habana, 10400, La Habana, Cuba

    Yaimara Solís, Natalia Davidenko, Jeny Cruz & Carlos Peniche

  2. Department of Materials Science and Metallurgy, University of Cambridge, Cambridge, CB2 3QZ, UK

    Natalia Davidenko & Ruth E. Cameron

  3. Instituto de Cerámica y Vidrio, CSIC, 28049, Madrid, Spain

    Raúl G. Carrodeguas

  4. Facultad de Química, Universidad de La Habana, 10400, La Habana, Cuba

    Andy Hernández, Miriela Tomás & Carlos Peniche

Authors
  1. Yaimara Solís
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  2. Natalia Davidenko
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  3. Raúl G. Carrodeguas
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  4. Jeny Cruz
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  5. Andy Hernández
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  6. Miriela Tomás
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  7. Ruth E. Cameron
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  8. Carlos Peniche
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Correspondence to Carlos Peniche.

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Solís, Y., Davidenko, N., Carrodeguas, R.G. et al. Preparation, characterization, and in vitro evaluation of nanostructured chitosan/apatite and chitosan/Si-doped apatite composites. J Mater Sci 48, 841–849 (2013). https://doi.org/10.1007/s10853-012-6804-5

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  • DOI: https://doi.org/10.1007/s10853-012-6804-5

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