Summary
The use of the immuno-histochemical method permits the localization of aldolase isozymes in tissue sections. Upon incubating a section with a monomer-specific antiserum, isozymes containing that monomer remain in the section, whereas other cytoplasmic enzymes diffuse out of the section. If soluble antigen is added subsequently, it is bound by the tissue-bound antibody. These antibody fixed aldolases can then be stained by the use of a tetrazolium test linked to substrate hydrolysis.
In this way it was demonstrated that isozymes of aldolase containing mostly the A monomer are predominantly localized in the distal tubules, the collecting tubules, the vessels and capillaries of the kidney, the ganglia, the Purkinje cells, the neurons, the white matter and the chorioid plexus of the brain. Aldolase containing mostly B-monomers were found in the proximal tubules. Aldolase isozymes particularly rich in C-monomers were seen in the nervus opticus, the pia mater, the vessels of cerebrum and the molecular layer of the cortex cerebelli.
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References
Arnold, F., Kunze, K. D., Simon, H.: Zur Problematik des histochemischen Nachweises der Fruktose-1,6-Diphosphat-Aldolase am Beispiel der Rattenniere. Histochemie 9, 84–92 (1967)
Blostein, R., Rutter, W. J.: Comparative studies of liver and muscle aldolase. II. Immunochemical and chromatographic differentiation. J. biol. Chem. 238, 3280–3285 (1963)
Frunder, H.: Glycolysis in the liver under post-absorptive conditions. Folia haemat. 89, 414–417 (1968)
Lebherz, H. G., Rutter, W. J.: Distribution of fructose diphosphate aldolase variants in biological systems. Biochemistry (Wash.) 8, 109–121 (1969)
Penhoet, E., Kochman, M., Rutter, W. J.: Molecular and catalytic properties of aldolase C. Biochemistry (Wash.) 8, 4396–4402 (1969)
Penhoet, E., Rajkumar, T., Rutter, W. J.: Multiple forms of fructose diphosphate aldolase in mammalian tissue. Proc. nat. Acad. Sci. (Wash.) 56, 1275–1282 (1966)
Penhoet, E., Kochman, M., Valentine, R., Rutter, W. J.: The subunit structure of mammalian fructose diphosphate aldolase. Biochemistry (Wash.) 6, 2940–2949 (1967)
Pfleiderer, G., Dikow, A. L., Falkenberg, F.: Quantitative Bestimmung genetisch determinierter Isoenzyme mittels Immunitration — Verteilungsmusters der Aldolase A, B und C in menschlichen Organ- und Gewebeextrakten sowie in normalen und pathologischen Seren. Z. Physiol. Chem. 355, 233–238 (1974)
Prochazka, B., Wachsmuth, E. D.: Isozyme patterns of lactate dehydrogenase, creatine phosphokinase, phosphoglucomutase and aldolase of guinea pig tissues during ontogeny. J. exp. Zool. 182, 201–210 (1972)
Oliver, J., McDowell, M.: The structural and functional aspects of the handling of gluocse by the nephrons and the kidney and their correlation by means of structural-functional equivalents. J. clin. Invest. 40, 1093–1112 (1961)
Racker, E.: Spectrophotometric measurements of hexokinase and phosphokinase activity. J. biol. Chem. 167, 843–854 (1947)
Rensing, U., Schmid, A., Leuthardt, F.: Veränderungen im Isoenzymmuster der Aldolasen aus Rattenorganen während der Entwicklung. Z. Physiol. Chem. 348, 921–928 (1967)
Rutter, W. J., Blostein, R. E., Woodfin, B. M., Weber, C. S.: Enzyme variants and metabolic diversification. Advanc. Enzyme Reg. 1, 39–56 (1963)
Sigel, P., Pette, D.: Intracellular localization of glycogenolytic and glycolytic enzymes in white and red rabbit muscle. A gel film method for coupled enzyme reactions in histochemistry. J. Histochem. Cytochem. 17, 225–237 (1969)
Ullrich, K. J.: Das Nierenmark, Struktur, Stoffwechsel and Funktion. Ergebn. Physiol. 50, 434–489 (1959)
Wachsmuth, E. D.: An immuno-histochemical method for localization of enzymes in tissue sections: The use of antibody bound to tissue antigen and its property of binding crossreactive soluble antigen. Histochemie 37, 251–263 (1973)
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Part of this study was taken from the Ph.D. thesis of M. Thöner at the Ruhr-Universität, Bochum.
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Wachsmuth, E.D., Thöner, M. & Pfleiderer, G. The cellular distribution of aldolase isozymes in rat kidney and brain determined in tissue sections by the immuno-histochemical method. Histochemistry 45, 143–161 (1975). https://doi.org/10.1007/BF00495158
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DOI: https://doi.org/10.1007/BF00495158