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Ca2+-Dependent deposits at the plasmalemma of Chara internodal cells

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Summary

Electron dense deposits (EDD) were observed on the extracellular side of the plasmalemma of Chara internodal cells by a calcium-glutaraldehyde fixation technique. The number and size of EDD were greatly increased when cells had been preincubated in Ca2+-enriched medium before fixation. The addition of Na+, Mg2+, or La3+ instead of Ca2+ in incubation and fixation media produced no deposits at all, Sr2+ caused deposits with similar distribution to those formed by Ca2+, and Ba2+ addition resulted in deposits localized at different sites within the cell. Microprobe analysis of single EDD from Ca2+ incubated cells ascertained the presence of calcium in these deposits. Possible functions of the Ca2+-binding sites at the plasma membrane of Chara cells are discussed.

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  1. Botanisches Institut I der Universität Karlsruhe, Kaiserstrasse 2, D-7500, Karlsruhe, Germany

    H. -G. Heumann

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  1. H. -G. Heumann
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Heumann, H.G. Ca2+-Dependent deposits at the plasmalemma of Chara internodal cells. Histochemistry 79, 69–75 (1983). https://doi.org/10.1007/BF00494343

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  • DOI: https://doi.org/10.1007/BF00494343

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