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Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone

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Abstract

In molluscs, the neurotransmitter serotonin (5-HT) has been linked to a variety of biological roles including gamete maturation and spawning. The possible involvement of 5-HT in abalone gamete release was demonstrated by a dose-dependent increase in Haliotis rubra gonad contractile bioactivity following 5-HT stimulation. Physiological functions associated with 5-HT, are mediated through binding to 5-HT receptors. A cDNA encoding a putative 5-HT receptor consisting of 359 amino acids was isolated from the tropical abalone H. asinina, termed 5-HT1 ha. The 5-HT1 ha shares G-protein-coupled receptor motifs with metazoan 5-HT receptors, including predicted transmembrane domains, active sites for protein kinase action, and N-linked glycosylation sites. However, the third intracellular loop of 5-HT1 ha is relatively short, and only six transmembrane domains are predicted, implying a truncated receptor. Phylogenetic analysis with known 5-HT receptor genes suggests that 5-HT1 ha belongs to the type 1 5-HT receptor family. Expression analysis by RT-PCR showed that 5-HT1 ha mRNA was present in all tissues examined, including the neural ganglia and gonad tissues. Immunocytochemistry revealed the presence of 5-HT1 ha specifically within the soma of neuronal cells located in the outer cortex of both cerebral and pleuropedal ganglia. In ovarian and testicular tissues, 5-HT1 ha immunoreactivity was observed in epithelial cells of the outer capsule and connective tissue of the trabeculae to which the gamete follicles adhere. Whether this receptor transcript is translated to a functional protein needs to be verified, but if so, it could play a role in reproduction.

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Acknowledgments

We are grateful to Mr. Tanes Poomtong and staff at the Coastal Development Center, Klongwan, Prachaubkhirikhun, Thailand, and Mr. Tony Smith at the Bay City Seafarm, Geelong, Australia, for provision of animals. This work was supported by the Thailand Research Fund (Royal Golden Jubilee Ph.D. Program, Grant No. PHD/0150/2543 to S. Pansophonkul and P. Sretarugsa, and Senior Research Scholar Fellowship to P. Sobhon), a grant from Mahidol University to P. Sobhon, a University of Queensland Fellowship to S. F. Cummins, and ARC grants to B. M. Degnan.

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Authors and Affiliations

  1. Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand

    Sasiporn Panasophonkul, Somjai Apisawetakan, Peter J. Hanna, Porncharn Saitongdee, Prasert Sobhon & Prapee Sretarugsa

  2. School of Life and Environmental Sciences, Deakin University, Geelong, VIC, 3217, Australia

    Peter J. Hanna

  3. School of Integrative Biology, University of Queensland, Brisbane, QLD, 4072, Australia

    Scott F. Cummins, Patrick S. York & Bernard M. Degnan

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  1. Sasiporn Panasophonkul
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  2. Somjai Apisawetakan
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Correspondence to Prapee Sretarugsa.

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Panasophonkul, S., Apisawetakan, S., Cummins, S.F. et al. Molecular characterization and analysis of a truncated serotonin receptor gene expressed in neural and reproductive tissues of abalone. Histochem Cell Biol 131, 629–642 (2009). https://doi.org/10.1007/s00418-009-0555-7

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