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Genetic controls of T cell-independent Thy-1 alloantibody responses

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Abstract

Early and late primary IgM antibody responses of mice to Thy-1.1 antigens showed different antigenic and cellular requirements. We studied genetic controls of the early primary responses, which could be induced by subcellular thymocyte antigens independently of host T-cell activity. All Thy-1.2 mouse strains of Igh a(BALB/c and BC8), Igh-V aCb(BAB14), Igh d(AKR/Cum), Igh j(CBA/J, C3H/HeN, C3H.SW, and C3H.JK), and Igh n(NZB) definitely responded early to Thy-1.1 antigens from AKR/J (Igh d), A.Thy-1.1 (Igh e), or B10.Thy-1.1 (Igh b) mice or SD rats, whereas all strains of Igh b(C57BL/6, C57BL/10, B10.D2, B10.BR, B10.A, CB20 and CWB), Igh c(DBA/2), Igh e(A/J), and Igh o(C.AL20) responded poorly to the same antigens. This contrasts with the observation that both strains of Igh j(C3H/HeN) and Igh b(B10.BR) responded well at later times. As was the case for late responses, the matching of H-2 between donor and recipient resulted in early responses of exceptional quality in high-responder strains. It was concluded that under the influence of H-2, whose incompatibility between donor and recipient partially interferes with responses, early but not late primary Thy-1.1-specific antibody responses are selectively controlled by Igh-V or closely linked Ir gene(s) as a new V Hmarker.

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Abbreviations

Tl:

T cell-independent

TD:

T cell-dependent

PFC:

plaque-forming cell(s)

Igh:

immunoglobulin heavy chain

V H :

variable region of heavy chain

C H :

constant region of heavy chain

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Author notes

  1. Ken-ichi Isobe

    Present address: ICRF Tumour Immunology Unit, Department of Zoology, University College London, London WCIE, England

Authors and Affiliations

  1. Departments of Immunology and Bacteriology, Nagoya University School of Medicine, 466, Nagoya, Japan

    Ken-ichi Isobe,  Izumi Nakashima, Fumihiko Nagase, Tomoaki Yoshida, Ko-ichi Ando, Reiko Inagi, Nobuo Kato & Kimiko Ohashi

Authors
  1. Ken-ichi Isobe
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  2. Izumi Nakashima
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  3. Fumihiko Nagase
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  4. Tomoaki Yoshida
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  5. Ko-ichi Ando
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  6. Reiko Inagi
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  7. Nobuo Kato
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  8. Kimiko Ohashi
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Isobe, Ki., Nakashima, I., Nagase, F. et al. Genetic controls of T cell-independent Thy-1 alloantibody responses. Immunogenetics 20, 331–340 (1984). https://doi.org/10.1007/BF00364214

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  • DOI: https://doi.org/10.1007/BF00364214

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