Abstract
A skin grafting protocol is described for finding H-Y antigenic variants. The method is applicable regardless of the location of the structural gene(s) for this antigen (X, Y, or autosomal). Use of this protocol revealed no evidence for H-Y antigenic variation between C57BL/6J and strains 129/J, A.BY/SnJ, C3H.SW/SnJ, and LP/J.
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Andrews, P. W. and Wachtel, S. S.: Rejection of C57BL/6 skin grafts by (C57BL/6 × Mus musculus castaneus)F1 hybrids. Transplantation 27: 43–44, 1979
Bailey, D. W. and Usama, B.: A rapid method of grafting skin on tails of mice. Transplant. Bull. 7: 424–425, 1960
Billingham, R. E. and Silvers, W. K.: Studies on the tolerance of the Y chromosome antigen in mice. J. Immunol. 85: 14–26, 1960
Eicher, E. M. and Washburn, L. L.: Assignment of genes to regions of mouse chromosomes. PNAS 75: 946–950, 1978
Eichwald, E. J. and Silmser, C. R.: Communication. Transplant. Bull. 2: 148–149, 1955
Fox, A. S.: Genetics of tissue specificity. Ann. N.Y. Acad. Sci. 73: 611–634, 1958
Goldberg, E. H., Boyse, E. A., Scheid, M., and Bennett, D.: Production of H-Y antibody by female mice that fail to reject male skin. Nature 238: 55–57, 1972
Gasser, D. L., Silvers, W. K., and Wachtel, S. S.: Reply to Hildemann. Science 185: 963, 1974
Hurme, M., Chandler, P. R., Hetherington, C. M., and Simpson, E.: Cytotoxic T-cell responses to H-Y: Correlation with the rejections of syngeneic male skin grafts. J. Exp. Med. 147: 768–775, 1978
Hildemann, W. H., Morgan, H., and Frautnick, L.: Immunogenetic components of weaker histocompatibility systems in mice. Transplant. Proc. 2: 24–31, 1970
Johnson, L. L., Bailey, D. W., and Mobraaten, L. E.: Genetics of histocompatibility in mice. II. Survey for interactions between minor (non-H-2) antigens by skin grafting. Immunogenetics 11: 363–372, 1980
Johnson, L. L., Bailey, D. W., and Mobraaten, L. E.: Genetics of histocompatibility in mice. Ill. Characteristics of the popliteal lymph-node response to minor (non-H-2) H antigens. Immunogenetics 13: 327–337, 1981
Koo, G. C. and Varano, A.: Inhibition of H-Y cell-mediated cytolysis by monoclonal H-Y specific antibody. Immunogenetics 14: 183–188, 1981
Králová, J. and Démant, P.: Expression of H-Y antigen on thymus and skin: differential genetic control linked to K end of H-2. Immunogenetics 3: 583–594, 1976
Králová, J. and Lengerová, A.: H-Y antigen: Genetic control of the expression as detected by host versus graft popliteal lymph node enlargement assays maps between the T and H-2 complexes. J. Immunogenetics 6: 429–438, 1979
Nichols, E. A. and Ruddle, F.: A review of enzyme polymorphism, linkage, and electrophoretic conditions for mouse and somatic cell hybrids in starch gels. J. Histochem. Cytochem. 21: 1066–1081, 1973
Ohno, S.: Major regulatory genes for mammalian sexual development. Cell 7: 315–321, 1976
Simpson, E. and Gordon, R.D.: H-Y antigen—Ir gene complementation and target cell specificity. Immunol. Rev. 35: 59–75, 1977
Simpson, E., Brunner, C., Chandler, P. R., Brenan, M., Dagg, M., and Bailey, D. W.: H-Y antigen: No evidence for alleles in wild strains of mice. Immunogenetics 8: 213–219, 1979
Simpson, E., McLaren, A., and Chandler, P.: Evidence for two male antigens in mice. Immunogenetics 15: 609–614, 1982
Snell, G. D. and Stimpfling, J. H.: Genetics of tissue transplantation. In E. L. Green (ed.): Biology of the Laboratory Mouse, pp. 457–491, Dover Puplications, New York, 1966
Vallera, D. A., Soderling, C. C. B., Carlson, C. J., and Kersey, J. H.: Bone marrow transplantation across major histocompatibility barriers in mice. Effect of elimination of T cells from donor grafts by treatment with monoclonal Thy-1.2 plus complement or antibody alone. Transplantation 31: 218–222, 1981
Wachtel, S. S.: Where is the H-Y structural gene? Cell 22: 3–4, 1980
Wachtel, S. S., Ohno, S., Koo, G. C., and Boyse, E. A.: Possible role for H-Y antigen in the primary determination of sex. Nature 257: 235–236, 1975
Zaalberg, O.B.: An analysis of the Eichwald-Silmser effect. Transplant. Bull. 6: 433–435, 1959
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Johnson, L.L. A protocol for detection of H-Y antigenic variants. Immunogenetics 16, 577–582 (1982). https://doi.org/10.1007/BF00372026
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DOI: https://doi.org/10.1007/BF00372026