Summary
By isoelectrofocusing in agarose, the properdin factor allotype BfF could be split into two subtypes: BfFa with one major cathodic band and BfFb with the same cathodic band but in addition a major anodic band. By scanning, BfFaFb heterozygotes were distinguished from BfFbFb homozygotes by the stronger intensity of the anodic band in the latter. The two subtypes were segregated perfectly with HLA in 40 families and showed different association patterns with HLA markers. BfFa seemed to be linked to B35 while BfFb showed a strong linkage with all the components of the following haplotype: HLA-A29, Cw-, B44, BfFb, C4A3B1, DR7. The frequency of BfFa among BfFS heterozygotes was 41% (46/113) and that of BfFb 59% (67/113).
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Albert ED, Rittner C, Scholz S, Kuntz B, Mickey MR (1977) Three point association of HLA-A,B,Bf haplotypes deduced in 200 parents of 100 families. Scand J Immunol 6:459–464
Alper CA, Boenisch T, Watson L (1972) Genetic polymorphism in human glycine-rich beta glycoprotein. J Exp Med 135:68–80
Awdeh ZL, Alper CA (1980) Inherited structural polymorphism of the fourth component of human complement. Proc Natl Acad Sci USA 77:3576–3580
Bender K, Mayerova A, Frank R, Hiller C, Wienker T (1977) Haplotype analysis of the linkage group HLA-A, HLA-B; Bf and its bearing on the interpretation of the linkage disequilibrium. Hum Genet 36:191–196
Carroll MC, Campbell RD, Bentley DR, Porter RR (1984) A molecular map of the human major histocompatibility complex class III region linking complement genes C4, C2 and factor B. Nature 307:237–241
David V, Fauchet R, Phengsavath H, Guenet L, Le Gall JY (1983) Properdin factor B(Bf) polymorphism: subtyping of SS phenotypes. Hum Genet 64:189–190
Geserick G, Patzelt D, Schröder H, Nagai T (1983) Isoelectrofocusing in the study of the Bf system: existence of two common subtypes of the Bf allele. Vox Sang 44:178–182
Hauptmann G, Sasportes M, Tongio M, Mayer S, Dausset J (1976a) The localization of the Bf locus within the MHS region of chromosome no 6. Tissue Antigens 7:52–54
Hauptmann G, Tongio MM, Mayer S (1976b) Bf polymorphism: study of a new variant (F0.55). Hum Genet 33:275–278
Hauptmann G, Wertheimer E, Tongio M, Mayer S (1977) Bf polymorphism: another variant (S0.8). Hum Genet 36:109–111
Hobart MJ, Lachmann PJ (1976) Allotypes of complement components in man. Transplant Rev 32:26–42
Lalanne JL, Cochet M, Kummer AM, Gachelin G, Kourilsky P (1983) Different exon-intron organization at the 5′ part of a mouse class I gene is used to generate a novel H-2Kd-related on RNA. Proc Natl Acad Sci USA 80:7561–7565
Mauff G, Hummel K, Pulverer G (1975) Properdin factor B (glycinerich beta-glycoprotein or C3 proactivator) polymorphism: genetic and biochemical aspects. First application to paternity cases. Immunobiology 150:327–336
O'Neill GJ, Miniter P, Nerl C, Young Yang S, Dupont B, Pollack MS (1982) The Bf locus and HLA: rare alleles coding for functionally active and inactive factor-B products. Hum Immunol 5:239–246
Raum D, Glass D, Carpenter CB, Alper CA, Schur Ph (1976) The chromosomal order of genes controlling the major histocompatibility complex, properdin factor B, and deficiency of the second component of complement. J Clin Invest 58:1240–1248
Robson EB, Lamm LU (1984) Report on the committee on the genetic constitution of chromosome 6. Cytogenet Cell Genet 37:47–70
Teisberg P, Olaisen B, Gedde-Dahl T, Thorsby E (1975) On the localization of the Gb locus within the MHS region of chromosome no6. Tissue Antigens 5:257–261
Teng YS, Tan SG (1982) Subtyping of properdin factor (Bf) by isoelectrofocusing. Hum Hered 32:362–366
Terasaki PI, McClelland JD (1964) Microdroplet assay of human serum cytotoxins. Nature 204:998–1000
Van Rood JJ, Van Leeuwen A, Ploem JS (1976) Simultaneous detection of two cell populations by two-colour fluorescence and application to the recognition of B-cell determinants. Nature 262:795–797
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Abbal, M., Thomsen, M., Cambon-Thomsen, A. et al. Two subtypes of BfF by isoelectrofocusing: Differential linkage to other HLA markers. Hum Genet 69, 181–183 (1985). https://doi.org/10.1007/BF00293294
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DOI: https://doi.org/10.1007/BF00293294