Abstract
The murine retinoid X receptor β (mRXRβ) is a nuclear hormone receptor that activates transcription of murine major histocompatibility complex (MHC) class I genes in response to retinoic acid. In this study, the human RXRβ gene was mapped onto the short arm or centromeric region of chromosome 6 (6pter-q13), which also harbors the MHC. Chromosomal localization was performed by Southern hybridization of genomic DNA from human rodent cell hybrids with the mRXRβ gene as a probe. In addition, a full-length cDNA clone encoding a human RXRβ was isolated by nucleic acid screening of a human cDNA library with a fragment of the mRXRβ gene as a probe. Comparison of the nucleotide-coding sequences of the human and the murine RXRβ revealed a predominance of third base substitutions, resulting in selective conservation of the predicted amino acid sequence of the proteins. The overall sequence homology was 97.6% on the amino acid level as opposed to 91.6% on the nucleotide level. In Northern hybridization experiments with the human cDNA as a probe, RXRβ gene transcripts were detected in a variety of human tumor cell lines, regardless of whether these cell lines expressed MHC class I genes.
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References
Beato M (1989) Gene regulation by steroid hormones. Cell 56:335–344
DiSanto JP, Knowles RW, Flomenberg N, Dupont B (1988) The human Lyt-3 molecule requires CD8 for cell surface expression. EMBO J 7:3465–3470
Fleischhauer K, Park JH, DiSanto JP, Marks MS, Ozato K, Yang SY (1992) Isolation of a full-length cDNA clone encoding a N-terminally variant form of the human retinoid X receptor β. Nucleic Acids Res 20:1801
Forman BM, Samuels HH (1990) Interactions among a subfamily of nuclear hormone receptors: The regulatory zipper model. Mol Endocrinol 4:1293–1301
Glass CK, Rosenfeld MG (1991) Regulation of gene transcription by thyroid hormones and retinoic acid. In: Foukes G, Cohen P (eds) Molecular aspects of cellular regulation: the hormonal control regulation of gene transcription. Elsevier, Amsterdam, pp 299–327
Green S, Chambon P (1988) Nuclear receptors enhance our understanding of transcription regulation. Trends Genet 4:309–314
Hamada K, Gleason SL, Levi BZ, Hirschfeld S, Appella E, Ozato K (1989) H-2RIIBP, a member of the nuclear hormone receptor superfamily that binds to both the regulatory element of major histocompatibility complex class I genes and the estrogen response element. Proc Natl Acad Sci USA 86:8289–8293
Kliewer SA, Umesono K, Mangelsdorf DJ, Evans RM (1992) Retinoid X receptor interacts with nuclear receptors in retinoic acid, thyroid hormone and vitamin D3 signalling. Nature 355:446–449
Leid M. Kastner P, Lyons R, Nakshatri H, Saunders M, Zacharewski T, Chen JY, Staub A, Garnier JM, Mader S, Chambon P (1992) Purification, cloning, and RXR identity of the HeLa cell factor with which RAR or TR heterodimerizcs to bind target sequences efficiently. Cell 68:377–395
Levin AA, Sturzenbecker LJ, Kazmer S, Bosakowski T, Huselton C, Allenby G, Speck J, Kratzeisen C, Rosenberger M, Lovey A, Grippo JF (1992) A 9-cis retinoic acid stereoisomer binds to and activates the nuclear receptor RXRα. Nature 355:359–361
Lotan R (1980) Effects of vitamin A and its analogs (retinoids) on renal and neoplastic cells. Biochim Biophys Acta 605:33–91
Mangelsdorf DJ, Ong ES, Dyck JA, Evans RM (1990) A nuclear receptor that identifies a novel retinoic acid response pathway. Nature 345:224–229
Mangelsdorf DJ, Borgmcyer U, Heyman RA, Zhou JY, Ong ES, Oro AE, Kakizuka A, Evans RM (1992) Characterization of three RXR genes that mediate the action of 9-cis retinoic acid. Genes Dev 6:329–344
Maniatis T, Fritsch EF, Sambrook J (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
Marks MS, Hallenbeck PL, Nagata T, Segars JH, Appella E, Nikodem VM, Ozato K (1992) H-2RIIBP (RXRβ) heterodimerization provides a mechanism for combinatorial diversity in the regulation of retinoic acid and thyroid hormone responsive genes. Embo J 11:1419–1435
McBride OW, Hieter P, Hollis GF, Swan DC, Otey M, Leder P (1982) Chromosomal location of human k and λ immunoglobulin light chain constant region genes. J Exp Med 155:1480–1490
McBride OW, Swan DC, Tonick S, Gol R, Klamanis D, Moore D, Aaronson S (1983) Regional chromosomal localization of N-ras K-ras-1 K-ras-2 and myb oncogenes in human cells. Nucleic Acids Res 11:8221–8236
McBride OW, Malini R, Hatfield D (1987) Opal suppressor phosphoserine tRNA gene and pseudogene are located on human chromosomes 19 and 22, respectively. J Biol Chem 262:11163–11166
Nagata T, Segars JH, Levi BZ, Ozato K (1992) Retinoic acid-dependent transactivation of major histocompatibility complex class I promoters by the nuclear hormone receptor H-2 RIIBP in undifferentiated embryonal carcinoma cells. Proc Natl Acad Sci USA 89:937–941
Oro AE, McKeown M, Evans RM (1990) Relationship between the product of the Drosophila ultraspiracle locus and the vertebrate retinoid X receptor. Nature 347:298–301
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Shea MJ, King DL, Conboy MJ, Mariani BD, Kafatos FC (1990) Proteins that bind to Drosophila chorion cis-regulatory elements: a new C2H2 zinc finger protein and a C2C2 steroid receptor-like component. Genes Dev 4:1128–1140
Silverman T, Rein A, Orrison B, Langloss J, Bratthauer G, Miyazaki JI, Ozato K (1988) Establishment of cell lines from somite stage mouse embryos and expression of major histocompatibility class I genes in these cells. J Immunol 140:4378–4387
Sporn MB, Roberts AB (1983) Role of retinoids in differentiation and carcinogenesis. Cancer Res 43:3034–3040
Umesono K, Murakemi KK, Thompson CC, Evans RM (1991) Direct repeats as selective response elements for the thyroid hormone, retinoic acid, and vitamin D3 receptors. Cell 65:1255–1266
Warrell RP, Frankel SR, Miller WH, Scheinberg DA, Itri LM, Hittelman WN, Vyas R, Andreef M, Tafuri A, Jakubowski A, Gabrilove J, Gordon MS, Dmitrovsky E (1991) Differentiation therapy of acute promyelocytic leukemia with tretinoin (alltrans-retinoic acid). N Engl J Med 324:1385–1393
Yamamoto KR (1985) Steroid receptor regulated transcription of specific genes and gene networks. Annu Rev Genet 19:209–252
Yu VC, Delsert C, Andersen B, Holloway JM, Devary OV, Näär AM, Kim SY, Boutin JM, Glass CK, Rosenfeld MG (1991) RXRβ: a coregulator that enhances binding of retinoic acid, thyroid hormone, and vitamin D receptors to their cognate response elements. Cell 67:1251–1266
Zhang XK, Hoffmann B, Tran PBV, Graupner G, Pfahl M (1992) Retinoid X receptor is an auxiliary protein for thyroid hormone and retoic acid receptors. Nature 355:441–446
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Fleischhauer, K., McBride, O.W., DiSanto, J.P. et al. Cloning and chromosome mapping of human retinoid X receptor β: selective amino acid sequence conservation of a nuclear hormone receptor in mammals. Hum Genet 90, 505–510 (1993). https://doi.org/10.1007/BF00217449
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DOI: https://doi.org/10.1007/BF00217449