Skip to main content
Log in

In situ mapping of the muscle-specific form of phosphoglycerate mutase gene to human chromosome 7p12-7p13

  • Short Communications
  • Published:
Human Genetics Aims and scope Submit manuscript

Summary

A 2.3-kb-long probe derived from the 5′ flanking region, the first exon and part of the first intron of the human muscle-specific phosphoglycerate mutase gene (PGAM-M) (EC 5.4.2.1) was used to map the gene by “in situ” chromosomal hybridization. The structural gene for PGAM-M was assigned to chromosome 7p12-7p13; a single hybridization peak indicated that there is a single gene for this isozyme of PGAM, and confirmed results obtained by Southern blot hybridization.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Barichard F, Joulin V, Henri I, Garel MC, Valentin C, Rosa R, Cohen-Solal M, Junien C (1987) Chromosomal assignment of the human 2,3-bisphosphoglycerate mutase gene (BPGM) to region 7q-34→7q22. Hum Genet 77:283–285

    Google Scholar 

  • Bartrons R, Carreras J (1982) Purification and characterization of phosphoglycerate mutase isozymes from pig heart. Biochim Biophys Acta 708:167–177

    Google Scholar 

  • Castella-Escola J, Ojcius DM, Le Boulch P, Joulin V, Blouquit Y, Garel MC, Valentin C, Rosa R, Climent-Romeo F, Cohen-Solal M (1989) Isolation and characterization of the gene coding for the muscle-specific isozyme of human phosphoglycerate mutase. Gene (accepted)

  • Di Mauro S, Miranda AF, Olarte M, Friedman R, Hays AP (1982) Muscle phosphoglycerate mutase deficiency. Neurology 32:584–591

    Google Scholar 

  • Dutrillaux B (1975) Sur la nature et l'origine des chromosomes humains. (Monographie des Annales de Génétique) L'Expansion Scientifique, Paris

    Google Scholar 

  • Fothergill-Gilmore (1986) The evolution of glycolytic pathway. Trends Biochem Sci 11:47–51

    Google Scholar 

  • Freytag SO, Bock HG, Beaudet AL, O'Brien WE (1984) Molecular structures of human arginosuccinate synthase pseudogenes. Evolutionary and mechanistic implications. J Biol Chem 259:3160–3166

    Google Scholar 

  • Human Gene Mapping 9 (1987) 9th International Workshop on Human Gene Mapping. Cytogenet Cell Genet 46:1–762

  • Junien C, Despoisse S, Turleau C, Grouchy J de, Bucher T, Fundele R (1982) Assignment of phosphoglycerate mutase (PGAMA) to human chromosome 10. Regional mapping of GOT1 and PGAMA to subbands 10q26. 1 (or q25. 3). Ann Génét (Paris) 25:25–27

    Google Scholar 

  • Le Boulch P, Joulin V, Garel MC, Rosa J, Cohen-Solal M (1988) Molecular cloning and nucleotide sequence of the murine 2,3-bisphosphoglycerate mutase cDNA. Biochem Biophys Res Commun 156:874–881

    Google Scholar 

  • Mattei MG, Philip N, Passage E, Moisan JP, Mandel JL, Mattei JF (1985) DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome. Hum Genet 69:268–271

    Google Scholar 

  • Mezquita J, Carreras J (1981) Phylogeny and ontogeny of the phosphoglycerate mutases. I. Electrophoretic phenotypes of the glycerate 2,3-P2 dependent phosphoglycerate mutase in vertebrates. Comp Biochem Physiol 70B:237–245

    Google Scholar 

  • Omnen GS, Hermodson MA (1975) Human phosphoglycerate mutase: isozyme markers for muscle differentiation and for neoplasia. In: Markert Ch (ed) Isozymes III: developmental biology. Academic Press, New York London, pp 1005–1018

    Google Scholar 

  • Sakoda S, Shanske S, Di Mauro S, Schon EA (1988) Isolation of a cDNA encoding the B isozyme of human phosphoglycerate mutase (PGAM) and characterization of the PGAM gene family. J Biol Chem 263:16899–16905

    Google Scholar 

  • Shanske S, Sakoda S, Hermodson MA, Di Mauro S, Schon EA (1987) Isolation of a cDNA encoding the muscle-specific subunit of human phosphoglycerate mutase. J Biol Chem 262:14612–14617

    Google Scholar 

  • Szabo P, Grzeschik KH, Siniscalco M (1984) A human autosomal phosphoglycerate kinase locus maps near the HLA cluster. Proc Natl Acad Sci USA 81:3167–3169

    Google Scholar 

  • Tsui LP (1988) Genetic markers on chromosome 7. J Med Genet 25:294–306

    Google Scholar 

  • Vandeberg JL, Cooper DW, Close PJ (1973) Mammalian testis phosphoglycerate kinase. Nature New Biol 243:48–50

    Google Scholar 

  • Zasloff M, Ginder GD, Felsenfeld G (1978) A new method for the purification and identification of covalently closed circular DNA molecules. Nucleic Acids Res 6:1139–1145

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Castella-Escola, J., Mattei, M.G., Ojcius, D.M. et al. In situ mapping of the muscle-specific form of phosphoglycerate mutase gene to human chromosome 7p12-7p13. Hum Genet 84, 210–212 (1990). https://doi.org/10.1007/BF00208945

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00208945

Keywords

Navigation