Abstract
The recovery in tension after release of a fibrillar muscle preparation as well as the fall in tension after restretch was found to be greater in presence of AMP-PNP than in its absence (rigor). The effect of AMP-PNP was concentration-dependent with an optimum at 0.1 mM corresponding to the dissociation constant of AMP-PNP from the myosin heads. This evidence supports the validity of the teinochemical principle which predicts a stretch-dependent AMP-PNP binding. The stiffness calculated per cross bridge was similar to that found by Huxley and Simmons (1971). It was further calculated that only 15% of the cross bridges are in a force-maintaining state in rigor.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abbott, R. H., Leech, A. R.: Persistance of adenylate kinase and other enzymes in glycerol extracted muscle. Pflügers Arch.344, 233–243 (1973)
Barrington Leigh, J., Holmes, K. C., Mannherz, H. G., Rosenbaum, G., Eckstein, F., Goody, R.: Effects of ATP analogs on the low-angle X-ray diffraction pattern of insect flight muscle. Cold Spr. Harb. Symp. quant. Biol.37, 443–447 (1973)
Beinbrech, G., Kuhn, H. J., Herzig, J. W., Rüegg, J. C.: Evidence for two attached myosin cross bridge states of different potential energy. Cytobiology12, 385–396 (1976)
Feldhaus, P., Fröhlich, T., Goody, R. S., Isakov, M., Schirmer, R. H.: Synthetic inhibitors of adenylate. kinases in the assays for ATPases and phosphokinases. Europ. J. Biochem.57, 197–204 (1975)
Ford, L. E., Huxley, A. F., Simmons, R. M.: Tension responses to sudden length change in stimulated frog muscle fibres near slack length. J. Physiol. (Lond.)269, 441–515 (1977)
Goody, R. S., Holmes, K. C., Mannherz, H. G., Barrington Leigh, J., Rosenbaum, G.: X-ray diffraction studies of insect flight muscle with ATP analogues. Biophys. J.15, 687–704 (1975)
Goody, R. S., Barrington Leigh, J., Mannherz, H. G., Tregear, R. T., Rosenbaum, G.: X-ray titration of bindingΒ,γ-imido-ATP to myosin in insect flight muscle. Nature (Lond.)262, 613–614 (1976)
Güth, K., Kuhn, H. J.: Slippage of attached cross bridges in contracting rabbit psoas preparations induced by rapid lenght changes. Biophys. Struct. Mechanism4, 223–236 (1978)
Hill, T. L.: Theoretical formalism for the sliding filament model of contraction of striated muscle, Part I. Progr. Biophys. molec. Biol.28, 267–340 (1974)
Holmes, K. C.: The myosin cross-bridge as revealed by structure studies. In: Int. Symp. Rottach-Egern/Tegernsee, 1976 (eds. G. Riecker, A. Weber, J. Goodwin). Berlin-Heidelberg-New York Springer 1977
Huxley, A. F., Simmons, R. M.: Proposed mechanism of force generation in striated muscle. Nature (Lond.)233, 533–538 (1971)
Huxley, A. F.: Muscular contraction. J. Physiol. (Lond.)243, 1–43 (1974)
Huxley, H. E.: The mechanism of muscular contraction. Science164, 1356–1366 (1969)
Julian, F. J., Sollins, K. R., Sollins, M. R.: A model for the transient and steady-state mechanical behaviour of contracting muscle. Biophys. J.14, 546–562 (1974)
Kuhn, H. J.: Transformation of chemical energy into mechanical energy by glycerol-extracted fibres of insect flight muscle in the absence of nucleosidetriphosphate-hydrolysis. Experientia (Basel)29, 1086–1088 (1973)
Kuhn, H. J.: Reversible transformation of mechanical work into chemical free energy by stretch dependent binding of AMP-PNP in glycerinated fibrillar muscle fibres. In: Symposium on insect flight muscle, Oxford 1977 (ed. R. T. Tregear). Amsterdam: Elsevier/North Holland 1977a
Kuhn, H. J.: Cross bridge slippage induced by the ATP analogue AMP-PNP and stretch in glycerol-extracted fibrillar muscle fibres. Biophys. Struct. Mechanism4, 159–168 (1978)
Kuhn, W., Ramel, A., Walters, D. H., Ebner, G., Kuhn, H. J.: The production of mechanical energy from different forms of chemical energy with homogeneous and cross-striated high polymer systems. Fortschr. Hochpolymer-Forschg.1, 540–592 (1960)
Kuhn, W., Müller, S., Kuhn, H. J., Eisenberg, A.: Experimentelle Prüfung der Beziehungen zwischen mechanischen Konstanten von Gelen für Dehnung bei konstantem Quellungsgrade einerseits, bei konstantem Partialdruck des Quellungsmittels andererseits. Makromol. Chem.62, 40–48 (1963)
Lymn, R. W., Taylor, E. W.: Mechanism of adenosine triphosphate hydrolysis by actomyosin. Biochemistry (Wash.)10, 4617–4624 (1971)
Lymn, R. W., Huxley, H. E.: X-ray diagrams from skeletal muscle in the presence of ATP-analogs. Cold Spr. Harb. Symp. quant. Biol.37, 449–453 (1973)
Marston, S. B., Rodger, C. D., Tregear, R. T.: Changes in muscle cross bridges whenΒ,γ-imido-ATP binds to myosin. J. molec. Biol.104, 263–276 (1976)
Podolsky, R. J., Nolan, A. C.: Muscle contraction transients, cross bridge kinetics, and the Fenn effect. Cold Spr. Harb. Symp. quant. Biol.37, 661–668 (1973)
Reedy, M. K., Holmes, K. C., Tregear, R. T.: Induced changes in orientation of the cross bridges of glycerinated insect flight muscle. Nature (Lond.)207, 1276–1280 (1965)
Reedy, M. K.: Ultrastructure of insect flight muscle. I. Screw sense and structural grouping in the rigor cross bridge lattice. J. molec. Biol.31, 155–176 (1968)
Rüegg, J. C., Kuhn, H. J., Herzig, J. W., Dickhaus, H.: Effect of calcium ions on force generation and elastic properties of briefly glycerinated muscle fibres. In: Calcium transport in contraction and secretion (eds. E. Carafoli et al.). Amsterdam: North-Holland 1975
Schaub, M. C., Watterson, J. G., Pfister, M., Waser, P. G.: Ligand induced changes in thiol group reactivity of fast and slow myosins. FEBS-Symp.31, 21–30 (1975)
Tregear, R. T., Squire, J. M.: Myosin content and filament structure in smooth muscle. J. molec. Biol.77, 279–290 (1973)
White, D. C. S.: Rigor contraction in glycerinated insect flight and vertebrate muscle. J. Physiol. (Lond.)208, 583–605 (1970)
White, H. D., Taylor, E. W.: Energetics and mechanism of actomyosin adenosine triphosphate. Biochemistry (Wash.)15, 5818–5826 (1976)
Yount, R., Babcock, D., Ballantyne, W., Ojala, D.: Adenyl imidodiphosphate, an adenosine triphosphate analog containing a P-N-P linkage. Biochemistry (Wash.)10, 2484–2489 (1971)
Yamamoto, T., Herzig, J. W.: Series elastic properties of skinned muscle fibres in contraction and rigor. Pflügers Arch, (in press)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kuhn, H.J. Tension transients in fibrillar muscle fibres as affected by stretch-dependent binding of AMP-PNP: A teinochemical effect?. Biophys. Struct. Mechanism 4, 209–222 (1978). https://doi.org/10.1007/BF02426086
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02426086