Skip to main content
SpringerLink
Log in

Enzymic synthesis, chemical characterisation and Sda activity of GalNAcß1-4[NeuAcα2-3]Galß1-4GlcNAc and GalNAcß1-4[NeuAcα2-3]Galß1-4Glc

  • Published:
Glycoconjugate Journal Aims and scope Submit manuscript

Abstract

The tetrasaccharides GalNAcß1-4[NeuAcα2-3]Galß1-4Glc and GalNAcß1-4[NeuAcα2-3]Galß1-4GlcNAc were synthesised by enzymic transfer of GalNAc from UDP-GalNAc to 3′-sialyllactose (NeuAcα2-3Galß1-4Glc) and 3′-sialyl-N-acetyllactosamine (NeuAcα2-3Galß1-4GlcNAc). The structures of the products were established by methylation and1H-500 MHz NMR spectroscopy. In Sda serological tests the product formed with 3′-sialyl-N-acetyllactosamine was highly active whereas that formed with 3′-sialyllactose had only weak activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Race RR, Sanger R (1975) Blood Groups in Man, 6th edition, BlackwellOxford, p 400–5.

    Google Scholar 

  2. Bird GWG (1952) Nature 170:674.

    PubMed  Google Scholar 

  3. Cazal P, Monis M, Caubel J, Brives J (1968) Rev Franc Transf 11:209–21.

    Google Scholar 

  4. Sanger R, Gavin J, Tippett P, Teesdale P, Eldon K (1971) Lancet i:1130.

    Google Scholar 

  5. Bird GWG, Wingham J (1971) Vox Sang 120:55–61.

    Google Scholar 

  6. Morton JA, Pickles MM, Terry AM (1970) Vox Sang 19:472–82.

    PubMed  Google Scholar 

  7. Soh CPC, Morgan WTJ, Watkins WM, Donald ASR (1980) Biochem Biophys Res Commun 93:1132–39.

    PubMed  Google Scholar 

  8. Cartron JP, Kornprobst M, Lemonnier M, Lambin P, Piller F, Salmon C (1982) Biochem Biophys Res Commun 106:331–37.

    PubMed  Google Scholar 

  9. Donald ASR, Soh CPC, Watkins WM, Morgan WTJ (1982) Biochem Biophys Res Commun 104:58–65.

    PubMed  Google Scholar 

  10. Etzler ME, Kabat EA (1970) Biochemistry 9:869–77.

    PubMed  Google Scholar 

  11. Donald ASR, Yates AD, Soh CPC, Morgan WTJ, Watkins WM (1983) Biochem Biophys Res Commun 115:625–31.

    PubMed  Google Scholar 

  12. Cartron JP, Blanchard D (1982) Biochem J 207:497–504.

    PubMed  Google Scholar 

  13. Blanchard D, Cartron JP, Fournet B, Montreuil J, van Halbeek H, Vliegenthart JFG (1983) J Biol Chem 258:7691–95.

    PubMed  Google Scholar 

  14. Blanchard D, Cartron JP, Fournet B, Leroy Y, Montreuil J (1983) Proc 7th Symp Glycoconjugates, eds Chester A, Heinegård D, Lundblad A, Svensson Lund, p 435.

  15. Serafini-Cessi F, Dall'Olio F (1983) Biochem J 215:483–89.

    PubMed  Google Scholar 

  16. Serafini-Cessi F, Dall'Olio F, Malagolini N (1986) Carbohydr Res 151:65–76.

    PubMed  Google Scholar 

  17. Carlson DM, Swanson AL, Roseman S (1964) Biochemistry 3:402–5.

    Google Scholar 

  18. Tamm I, Horsfall FL (1950) Proc Soc Exp Biol Med 74:108–14.

    Google Scholar 

  19. Donald ASR, Feeney J, (1986) Biochem J 236:821–28.

    PubMed  Google Scholar 

  20. Parkkinen J, Finne J (1983) Eur J Biochem 136:355–61.

    PubMed  Google Scholar 

  21. Kuhn R, Baer HH, Seelinger A (1958) Liebigs Ann 611:236–41.

    Google Scholar 

  22. Watkins WM, Morgan WTJ (1976) J Immunogenet 3:15–27.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Immunochemical Genetics, MRC Clinical Research Centre, Watford Road, HA1 3UJ, Harrow, Middlesex, U.K.

    Cecilia PC Soh, Alastair SR Donald, Walter TJ Morgan & Winifred M Watkins

  2. Blomedical Nuclear Magnetic Resonance Centre, National Institute for Medical Research, Mill Hill, NW7 1AA, London, U.K.

    James Feeney

Authors
  1. Cecilia PC Soh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alastair SR Donald
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. James Feeney
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Walter TJ Morgan
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Winifred M Watkins
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Soh, C.P., Donald, A.S., Feeney, J. et al. Enzymic synthesis, chemical characterisation and Sda activity of GalNAcß1-4[NeuAcα2-3]Galß1-4GlcNAc and GalNAcß1-4[NeuAcα2-3]Galß1-4Glc. Glycoconjugate J 6, 319–332 (1989). https://doi.org/10.1007/BF01047851

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01047851

Key words

Search

Navigation