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Characterization of trembling aspen wood (Populus tremuloides L.) degraded with the white rot fungus Ceriporiopsis subvermispora and MWLs isolated thereof

Untersuchung von durch den Weißfäulepilz (Ceriporiopsis subvermispora) abgebautem Espenholz (Populus tremuloides L.) und daraus isolierten Milled Wood Ligninen (MWLs)

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Abstract

Trembling aspen wood (Populus tremuloides L.) was treated with white rot fungus Ceriporiopsis subvermispora for 1, 2, 4, and 6 weeks. As fungal decay advanced, lignin contents were decreased gradually up to ca. 27.5% (based on the Klason residues of the control) after 6 weeks. Alkali solubility of cell wall residues was increased until 4 weeks of fungal treatment, but additional treatment did not cause any effects. Milled wood lignins (MWLs) were isolated from the decayed woods by Björkman’s procedure and subjected to thioacidolysis and analytical pyrolysis to investigate the modification of lignin structures during fungal degradation. Thioacidolysis revealed that the yields of trithioethylated C6C3 monomers, as a parameter for frequency of β-O-4 linkages in lignin, were substantially reduced (-20%, based on the control) in MWLs isolated from decayed woods. Analytical pyrolysis revealed that the relative amounts of coniferyl alcohol and sinapyl alcohol in the pyrolysates were lowered dependent on the biodegradation time, whereas an elevation of C6C1 and C6C2 pyrolytic phenols was observed. The results from both analytical methods strongly suggested that β-O-4 linkages were cleaved by C. subvermispora. Specially, degradation of syringyl-type lignin seems to be preferred.

Zusammenfassung

Espenholz (Populus tremuloides L.) wurde für eine Dauer von 1, 2, 4 und 6 Wochen dem Weißfäulepilz Ceriporiopsis subvermispora ausgesetzt. Nach sechswöchigem biologischem Abbau verringerte sich der Ligningehalt der Holzproben allmählich bis auf ca. 27.5% des ursprünglichen Klason-Ligningehaltes, wohingegen die MeOH-Extraktstoffe erheblich zunahmen. Bis zu einer Inkubationszeit von vier Wochen stieg die Alkalilöslichkeit der Zellwandsubstanzen an; eine längere Abbaudauer zeigte jedoch keine weiteren Auswirkungen. Aus dem abgebauten Holz wurden nach dem Björkman-Verfahren Milled Wood Lignine (MWLs) isoliert und mittels Thioacidolyse und analytischer Pyrolyse wurde die Veränderung der Ligninstruktur durch die Pilzeinwirkung untersucht.

Die Thioacidolyse zeigte, dass sich die relativen Mengen von C6C3 Trithioethylat-Monomeren, die als Parameter für die Häufigkeit der β-O-4 Bindungen im Lignin dienen, aufgrund der enzymatischen Aktivität des Pilzes um 20% verringert hatten.

Die relativen Mengen von Coniferyl- und Sinapylalkohol in den Pyrolyseprodukten der MWLs sanken mit der Dauer des biologischen Abbaus, wohingegen gleichzeitig eine deutliche Zunahme der Phenole des Typs C6C1 und C6C2 beobachtet werden konnte.

Die Ergebnisse beider Abbaumethoden deuten darauf hin, dass C. subvermispora die β-O-4 Bindungen, insbesondere die des Syringyl-Lignins, spaltet.

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Authors and Affiliations

  1. Division of Wood Chemistry and Microbiology, Korea Forest Research Institute, 130-712, Seoul, Korea

    Joon-Weon Choi, Don-Ha Choi, Sung-Suk Lee & Myung-Kil Kim

  2. Division of Life and Environmental Resources, College of Natural Resources, Daegu University, 712-714, Kyungsan, Korea

    Sye-Hee Ahn

  3. Institute of Wood Chemistry and Chemical Technology of Wood, Federal Research Center for Forestry and Forest Products (BFH), 21031, Hamburg, Germany

    Dietrich Meier & Oskar Faix

  4. Faculty of Paper Science and Engineering, College of Environmental Science and Forestry, State University of New York, 13210, Syracuse, New York, USA

    Gary M. Scott

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  1. Joon-Weon Choi
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Correspondence to Don-Ha Choi.

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Choi, JW., Choi, DH., Ahn, SH. et al. Characterization of trembling aspen wood (Populus tremuloides L.) degraded with the white rot fungus Ceriporiopsis subvermispora and MWLs isolated thereof . Holz Roh Werkst 64, 415–422 (2006). https://doi.org/10.1007/s00107-006-0133-9

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