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Differential inflammatory status in rats susceptible or resistant to diet-induced obesity: effects of EPA ethyl ester treatment

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Abstract

Background

Obesity has been associated with a chronic low degree inflammatory response, characterized by an increase of inflammatory adipocytokines like tumoral necrosis factor-α (TNF-α), interleukin-6 (IL-6) as well as the synthesis of acute phase reactants such as haptoglobin.

Aim of the study

To evaluate if impairments in the inflammatory response at the white adipose tissue (WAT) level could be involved in the mechanisms conferring susceptibility or resistance to high-fat diet-induced obesity (DIO).

Methods

The expression levels of WAT genes and systemic markers related to inflammation were evaluated in two groups of rats fed with a high-fat diet during 15 days that showed either an early susceptibility (DIO) or resistance (DR) to develop obesity. We also tested the efficacy of the eicosapentaenoic (EPA) ω-3 fatty acid treatment (35 days) to potentially counteract the obesity-associated inflammatory features in DIO rats.

Results

This trial showed that high-fat diet induces an increase on mRNA levels on TNF-α and haptoglobin in DIO animals (P < 0.05), while no significant changes were observed on DR rats. Furthermore, a significant increase in IL-6 mRNA (P < 0.05) was found in both DR and DIO rats. EPA-treatment caused a significant decrease in IL-6 mRNA (P < 0.05), without significant changes in haptoglobin mRNA levels in adipose tissue. An unexpected decrease was observed in haptoglobin serum levels (P < 0.05) in DIO rats, which was reverted to control values in EPA-treated animals.

Conclusions

Our data suggest that obesity susceptibility or resistance may depend on the genetic make up related to inflammatory features, and support a role for ω-3 fatty acids in the prevention of obesity-associated inflammation in adipose tissue. In addition, our data do not support the hypothesis that serum haptoglobin is an acute phase protein expected to be positively related to increased adiposity in rats, at least in early and medium stages of DIO.

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Acknowledgments

This work has been supported in part by Ministerio de Educación y Ciencia of Spain (AGL 2006-04716/ALI), by the Government of Navarra (Department of Education and Culture) and Línea especial de Investigación “Nutrición, Obesidad y Salud” (University of Navarra/LE/97). N. Pérez-Echarri was supported by a doctoral grant from Government of Navarra (Department of Education). The expert technical assistance of A. Lorente and V. Ciaurriz is gratefully acknowledged. EPA was generously provided by Brudy S.L (Spain).

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Authors and Affiliations

  1. Dept. of Nutrition and Food Sciences, Physiology and Toxicology, University of Navarra, 31008, Pamplona, Spain

    Nerea Pérez-Echarri, Patricia Pérez-Matute, Beatriz Marcos-Gómez, Maria J. Baena, Amelia Marti, J. Alfredo Martínez & María Jesus Moreno-Aliaga

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  1. Nerea Pérez-Echarri
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  2. Patricia Pérez-Matute
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  3. Beatriz Marcos-Gómez
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  4. Maria J. Baena
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  5. Amelia Marti
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  6. J. Alfredo Martínez
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  7. María Jesus Moreno-Aliaga
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Correspondence to María Jesus Moreno-Aliaga.

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Pérez-Echarri, N., Pérez-Matute, P., Marcos-Gómez, B. et al. Differential inflammatory status in rats susceptible or resistant to diet-induced obesity: effects of EPA ethyl ester treatment. Eur J Nutr 47, 380–386 (2008). https://doi.org/10.1007/s00394-008-0738-3

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  • DOI: https://doi.org/10.1007/s00394-008-0738-3

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