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UVA and UVB Penetration in the Water Column of a South West Atlantic Warm Temperate Estuary and its Effects on Cells and Fish Larvae

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Abstract

The objective of this study was to determine the seasonal incidence of ultraviolet A (UVA) and ultraviolet B (UVB) in the water column of the Patos Lagoon estuary and the effects of UV radiation (UVR) at different organizational levels. Field work was conducted to collect UVA and UVB radiance, transparency, salinity, seston and chlorophyll a (Chl a) concentration, and fish larvae at an inner and at an outer sampling site in the Patos Lagoon estuary (32°S). Cell culture techniques combined with tools for fluorimetric detection were used to assess possible damage caused by UVR. GEM-81 erythrophoroma cells and larvae of silverside Odontesthes argentinensis were used as biological indicators. Field results revealed that at the inner station, UVA penetration was deeper in autumn and shallower in spring when water-column was less transparent, fresher, and had the highest concentration of seston and Chl a. At the outer station, UVA penetration in the water-column in spring was significantly different than in summer, when transparency and water salinity were high and Chl a was low. UVB did not present significant variation between seasons at both sampling stations. Experiments with GEM-81 cell lineage revealed that UVA doses similar to the environment (1.35 J cm−2) inhibited cell proliferation, increased the concentration of reactive oxygen species (ROS) and the lipid peroxide content (LPO), and lowered the activity of the antioxidant defense system (total antioxidant capacity against peroxyl radicals (TOSC)). For UVB, 0.075 J cm−2 caused cytotoxicity and 0.015 J cm−2 induced DNA damage in cells. Silverside larvae showed low growth rate when exposed to UVA and low antioxidant capacity when exposed to UVB. These results suggest that planktonic organisms that live close to surface water column in the Patos Lagoon estuary are subject to the effects of continued exposure to UVR. Knowledge of this impact is relevant to understand future changes in the populations dynamics of planktonic organisms and its ecological and economic implications to southern Brazil.

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References

  • Abreu, P.C., M. Bergesch, L.A. Proença, C.A.E. Garcia, and C. Odebrecht. 2009. Short- and long-term chlorophyll a variability in the shallow microtidal Patos Lagoon estuary, Southern Brazil. Estuaries and Coasts. doi:10.1007/s12237-009-9181-9.

    Google Scholar 

  • Amado, L.L., M.L. Garcia, P.B. Ramos, R.F. Freitas, B. Zafalon, J.L.R. Ferreira, J.S. Yunes, and J.M. Monserrat. 2009. A method to measure total antioxidant capacity against peroxyl radicals in aquatic organisms: Application to evaluate microcystins toxicity. Science of the Total Environment 407: 2115–2123.

    Article  CAS  Google Scholar 

  • Armstrong, T.N., R. Reimschuessel, and B.P. Bradley. 2002. DNA damage, histological changes and DNA repair in larval Japanese medaka (Oryzias latipes) exposed to ultraviolet–B radiation. Aquatic Toxicology 58: 1–14.

    Article  CAS  Google Scholar 

  • Bantseev, V., and H.Y. Youn. 2006. Mitochondrial "movement" and lens optics following oxidative stress from UV-B irradiation: Cultured bovine lenses and human retinal pigment epithelial cells (ARPE-19) as examples. Annals of the New York Academy of Sciences 1091: 17–33.

    Article  CAS  Google Scholar 

  • Bell, G.M., and W.S. Hoar. 1950. Some effects of ultraviolet radiation on sockeye salmon eggs and alevins. Canadian Journal of Research 28: 35–43.

    Article  CAS  Google Scholar 

  • Belzile, C., S. Demers, G.A. Ferreyra, I. Schloss, C. Nozais, K. Lacoste, B. Mostajir, S. Roy, M. Gosselin, E. Pelletier, S.M.F. Gianesella, and S. Vernet. 2006. UV effects on marine planktonic food webs: a synthesis of results from mesocosm studies. Photochemistry and Photobiology 82: 850–856.

    Article  CAS  Google Scholar 

  • Blaustein, A.R., P.D. Hoffman, D.G. Hokit, J.M. Kiesecker, S.C. Walls, and J.B. Hays. 1994. UV repair and resistance to solar UV-B in amphibian eggs: a link to population declines? Proceedings of the National Academy of Sciences USA 91: 1791–1795.

    Article  CAS  Google Scholar 

  • Bothwell, M.L., D.M.J. Sherot, and C.M. Pollock. 1994. Ecosystem response to solar ultraviolet-B radiation: Influence of trophic-level interactions. Science 265: 97–100.

    Article  CAS  Google Scholar 

  • Calazans, D. 1984. Penetração de pós-larva do camarão-rosa Penaeus paulensis no estuário da Lagoa dos Patos, in: Resumos do II Simpósio Brasileiro sobre Recursos do Mar, Rio de Janeiro.

  • Conde, D., L. Aubriot, and R. Sommaruga. 2000. Changes in UV penetration associated with marine intrusions and freswater discharge in a shallow coastal lagoon of the Southern Atlantic Ocean. Marine Ecology Progress Series 207: 19–31.

    Article  Google Scholar 

  • Conde, D., L. Aubriot, S. Bonilla, and R. Sommaruga. 2002. Marine intrusions in a coastal lagoon enhance the negative effect of solar UV radiation on phytoplankton photosynthetic rates. Marine Ecology Progress Series 240: 57–70.

    Article  CAS  Google Scholar 

  • Crutzen, P.J. 1992. Ultraviolet on the increase. Nature 356: 104–105.

    Article  Google Scholar 

  • Cullen, J.J., and P.J. Neale. 1997. Biological weighting functions for describing the effects of ultraviolet radiation on aquatic systems, p. 97–118. In D.-P. Häder [ed.], Effects of ozone depletion on aquatic ecosystems. R. G. Landes.

  • Dahms, H.U., and J.S. Lee. 2010. UV radiation in marine ectotherms: Molecular effects and responses. Aquatic Toxicology 97: 3–14.

    Article  CAS  Google Scholar 

  • Dong, Q., K. Svoboda, T.R. Tiersch, and W.T. Monroe. 2007. Photobiological effects of UVA and UVB light in zebrafish embryos: Evidence for a competent photorepair system. Journal of Photochemistry and Photobiology B: Biology 88: 137–146.

    Article  CAS  Google Scholar 

  • Dyer, K.R. 1997. Estuaries: a physical introduction. WileyBlackwell, 2nd edition. p.195

  • Fernandes, E.H.L., I. Marino-Tapia, K.R. Dyer, and O.O. Möller. 2004. The attenuation of tidal and subtidal oscillations in the Patos Lagoon estuary. Ocean Dynamics 54(3–4): 348–359.

    Google Scholar 

  • Fidhiany, L., and K. Winckler. 1996. Combined effects of constant sublethal UVA irradiation and elevated temperature on the survival and general metabolism of the convict-cichlid fish, Cichlasoma nigrofasciatum. Photochemistry and Photobiology 63(4): 487–491.

    Article  Google Scholar 

  • Figueroa, F.L. 2002. Bio-optical characteristics of Gerlache and Bransfield Strait waters during an Antarctic summer cruise. Deep-Sea Research II 49: 675–691.

    Article  CAS  Google Scholar 

  • Gallagher, E.P., A.T. Canadá, and R.T. DiGiulio. 1992. The protective role of glutathione in chlorothalonil-induced toxicity to channel catfish. Aquatic Toxicology 23: 155–168.

    Article  CAS  Google Scholar 

  • Gao, K., G. Li, E.W. Helbling, and V.E. Villafañe. 2007. Variability of UVR effects on photosynthesis of summer phytoplankton assemblages from a tropical coastal area of the South China Sea. Photochemistry and Photobiology 83: 802–809.

    Article  CAS  Google Scholar 

  • Gouveia, G.R., D.S. Marques, B.P. Cruz, L.A. Geracitano, L.E.M. Nery, and G.S. Trindade. 2005. Antioxidant defenses and DNA damage induced by UV-A and UV-B radiation in the crab Chasmagnathus granulata (Decapoda, Brachyura). Photochemistry and Photobiology 81: 398–403.

    Article  CAS  Google Scholar 

  • Häder, D.P., and R.P. Sinha. 2005. Solar ultraviolet radiation-induced DNA damage in aquatic organisms: Potential environmental impact. Mutation Research 571: 221–233.

    Article  Google Scholar 

  • Häder, D.P., H.D. Kumar, R.C. Smith, and R.C. Worrest. 1998. Effects on aquatic ecosystems. Journal of Photochemistry and Photobiology B: Biology 46: 53–68.

    Article  Google Scholar 

  • Häder, D.P., E.W. Helbling, C.E. Williamson, and R.C. Worrest. 2011. Effects of UV radiation on aquatic ecosystems and interactions with climate change. Photochemical and Photobiological Sciences 10: 242–260.

    Article  Google Scholar 

  • Halliwell, B., and J.M.C. Gutteridge. 2007. Free radicals in Biology and Medicine. New York: Oxford University Press Inc.

    Google Scholar 

  • Hargreaves, B.R. 2003. Water column optics and penetration of UVR. In UV effects in aquatic organisms and ecosystems, ed. E.W. Helbling and H.E. Zagarese, 59–105. Cambridge: The Royal Society of Chemistry.

    Chapter  Google Scholar 

  • Hermes-Lima, M., W.G. Willmore, and K.B. Storey. 1995. Quantification of lipid peroxidation in tissue extracts based on Fe(III) xylenol orange complex formation. Free Radical Biology and Medicine 19: 271–280.

    Article  CAS  Google Scholar 

  • Hessen, D.O., E. Leu, P.J. Faerovig, and S.F. Petersen. 2008. Light and spectral properties as determinants of C:N:P-ratios in phytoplankton. Deep-Sea Research II: Topical Studies in Oceanography 55(20–21): 2169–2175.

    Article  CAS  Google Scholar 

  • Hofer, R., and C. Mokri. 2000. Photoprotection in tadpoles of the common frog, Rana temporaria. Journal of Photochemistry and Phobiology B: Biology 59: 48–53.

    Article  CAS  Google Scholar 

  • Hunter, J.R., J.H. Taylor, and H.G. Moser. 1979. Effect of ultraviolet irradiation on eggs and larvae of the Northern Anchovy, Engraulis mordax, and the Pacific mackerel, Scomber japonicus, during the embryonic stage. Photochemistry and Photobiology 29: 325–338.

    Article  CAS  Google Scholar 

  • Hunter, J.R., S.E. Kaupp, and J.H. Taylor. 1981. Effects of solar and artificial ultraviolet-B radiation on larval northern anchovy, Engraulis mordax. Photochemistry and Photobiology 34: 477–486.

    Article  Google Scholar 

  • Jerlov, N.G. 1950. Ultra-violet radiation in the sea. Nature 166: 111–112.

    Article  CAS  Google Scholar 

  • Karentz, D. 1994. Ultraviolet tolerance mechanisms in Antarctic marine organisms. In Ultraviolet radiation in antarctica: Measurements and biological effects, Antarctic Research Series, vol. 62, ed. C.S. Weiler and P.A. Penhale, 93–110.

    Chapter  Google Scholar 

  • Karentz, D.J., E.S. Mceuen, M.C. Land, and W.C. Dunlap. 1991. Survey of mycosporyne-like amino acidcompounds in Antarctic marine organisms: Potential protection from ultraviolet exposure. Marine Biology 108: 157–166.

    Article  CAS  Google Scholar 

  • Kerr, J.B. 1994. Decreasing ozone causes health concern. Environmental Science and Technology 28: 514–518.

    Article  Google Scholar 

  • Kerr, J.B., and C.T. McElroy. 1993. Evidence for large upward trends of ultraviolet-B radiation linked to ozone depletion. Science 262: 1032–1034.

    Article  CAS  Google Scholar 

  • Kirchhoff, V.W., N.J. Scuch, D.K. Pinheiro, and J.M. Harris. 1996. Evidence for an ozone hole perturbation at 30° South. Atmospheric Environmental 30: 1481–1488.

    Article  CAS  Google Scholar 

  • Kirk, J.T.O. 1994. Light and photosynthesis in aquatic ecosystems, 524. Cambridge: Cambridge University Press.

    Book  Google Scholar 

  • Kjerfve, B. 1986. Comparative oceanography of coastal lagoons. In Estuarine variability, ed. D.A. Wolfe, 63–81. New York: Academic.

    Chapter  Google Scholar 

  • Konca, K., A. Lankoff, A. Banasik, H. Lisowskab, T. Kuszewski, S. Gozdz, Z. Koza, and A. Wojcik. 2003. A cross-platform public domain PC image-analysis program for the comet assay. Mutation Research 534: 15–20.

    Article  CAS  Google Scholar 

  • Krapp, R.H., T. Baussant, J. Berge, D.M. Pampanin, and L. Camus. 2009. Antioxidant responses in the polar marine sea-ice amphipod Gammarus wilkitzkii to natural and experimentally increased UV levels. Aquatic Toxicology. doi:10.1016/j.aquatox.2009.05.005.

    Google Scholar 

  • Kuhn, P., H. Browman, B. McArthur, and J.F. St-Pierre. 1999. Penetration of ultraviolet radiation in the waters of the estuary and Gulf of St Lawrence. Limnology and Oceanography 44: 710–716.

    Article  CAS  Google Scholar 

  • Lesser, M.P. 2001. Exposure to ultraviolet radiation (290–400 nm) causes oxidative stress, DNA damage, and expression of p53/p73 in laboratory experiments on embryos of the spotted salamander, Ambystoma maculatum. Physiological and Biochemical Zoology 74: 733.

    Article  CAS  Google Scholar 

  • Lesser, M.P., J.H. Farrell, and C.W. Walker. 2001. Oxidative stress and p53 expression in the larvae of Atlantic cod (Gadus morhua) exposed to ultraviolet (290–400 nm) radiation. Journal of Experimental Biology 204: 157–164.

    CAS  Google Scholar 

  • Lubin, D., and E.H. Jensen. 1995. Effects of clouds and stratospheric ozone depletion on ultraviolet radiation trends. Nature 377: 710–713.

    Article  CAS  Google Scholar 

  • MacFadyen, E.J., C.E. Williamson, G. Grad, M. Lowery, W.H. Jeffrey, and D.L. Mitchell. 2004. Molecular response to climate change: Temperature dependence of UV-induced DNA damage and repair in the freshwater crustacean Daphnia pulicaria. Global Change Biology 10(4): 408–416.

    Article  Google Scholar 

  • Malloy, K.D., M.A.D. Holman, H.W. Mitchell, and I.I.I. Detrich. 1997. Solar UV-B induced DNA damage and photoenzymatic DNA repair in Antarctic zooplankton. Proceedings of the National Academy of Sciences USA 94: 1258–1263.

    Article  CAS  Google Scholar 

  • Manney, G.L., L. Froidevaux, J.W. Waters, R.W. Zurek, W.G. Read, L.S. Elson, J.B. Kumer, J.L. Mergenthaler, A.E. Roche, A. O’Niell, R.S. Harwood, I. MacKenzie, and R. Swinbank. 1994. Chemical depletion of ozone in the Arctic lower stratosphere during winter 1992–93. Nature 370: 429–434.

    Article  CAS  Google Scholar 

  • Martins, I.M., J.M. Dias, E.H. Fernandes, and J.H. Muelbert. 2007. Numerical modelling of fish eggs dispersion at the Patos Lagoon estuary—Brazil. Journal of Marine Systems 68: 537–555.

    Article  Google Scholar 

  • Misra, R.B., R.S. Ray, and R.K. Hans. 2005. Effect of UVB radiation on human erythrocytes in vitro. Toxicology in Vitro 19: 433–438.

    Article  CAS  Google Scholar 

  • Möller, O.O., J.A. Lorenzzentti, J.L. Stech, and M.M. Mata. 1996. The Patos Lagoon summertime circulation and dynamics. Continental Shelf Research 16(3): 335–351.

    Article  Google Scholar 

  • Möller, O.O., P. Castaing, J.-C. Solomon, and P. Lazure. 2001. The influence of local and non-local forcing effects on the subtidal circulation of Patos Lagoon. Estuaries 24(2): 297–311.

    Article  Google Scholar 

  • Muelbert, J.H., and G. Weiss. 1991. Abundance and distribution of fish larvae in the channel area of the Patos Lagoon Estuary, Brazil. In Larval fish recruitment and research in the Americas, NOAA Tech. Rep. NMFS, vol. 95, ed. R.D. Hoyt, 43–54.

    Google Scholar 

  • Obermüller, B., S. Puntarulo, and D. Abele. 2007. UV-tolerance and instantaneous physiological Gondogeneia antarctica and Djerboa furcipes stress responses of two Antarctic amphipod species during exposure to UV radiation. Marine Environmental Research 64: 267–285.

    Article  Google Scholar 

  • Odebrecht, C., P.C. Abreu, O.O. Möller, L.F. Niencheski, L.A. Proença, and L.C. Torgan. 2005. Drought effects on pelagic properties in the shallow and turbid Patos Lagoon, Brazil. Estuaries 5: 675–685.

    Article  Google Scholar 

  • Patton, W.P., U. Chakravarthy, R.J.H. Davies, and D.B. Archer. 1999. Comet assay of UV-Induced DNA damage in retinal pigment epithelial cells. Investigative Ophthalmology & Visual Science 40: 3268–3275.

    CAS  Google Scholar 

  • Piazena, H., and D.P. Häder. 1994. Penetration of solar UV irradiation in coastal lagoons of the Southern Baltic Sea and its effect on phytoplankton communities. Photochemistry and Photobiology 60: 463–469.

    Article  CAS  Google Scholar 

  • Punnonen, K., A. Puntala, and M. Ahotupa. 1991. UVB irradiation induces lipid-peroxidation and reduces antioxidant enzyme-activities in human keratinocytes in vitro. Acta Dermatol-Venereologica 71(3): 239–273.

    CAS  Google Scholar 

  • Ravanat, J.L., T. Douki, and J. Cadet. 2001. Direct and indirect effects of UV radiation on DNA and its components. Journal Photochemistry and Photobiology B: Biology 63: 88–102.

    Article  CAS  Google Scholar 

  • Ray, R.S., N. Agrawal, A. Sharma, and R.K. Hans. 2008. Use of L-929 cell line for phototoxicity assessment. Toxicology in Vitro 22: 1775–1781.

    Article  CAS  Google Scholar 

  • Sandrini, J.Z., G.S. Trindade, L.E.M. Nery, and L.F. Marins. 2009. Time-course expression of DNA repair-related genes in hepatocytes of zebrafish (Danio rerio) after UV-B exposure. Photochemistry and Photobiology 85: 220–226.

    Article  CAS  Google Scholar 

  • Scott, C.E., J.E. Saros, C.E. Williamson, C.R. Salm, S.C. Peters, and D.L. Mitchell. 2009. Effects of nutrients and dissolved organic matter on the response of phytoplankton to ultraviolet radiation: Experimental comparison in spring versus summer. Hydrobiologia 619: 155–166.

    Article  CAS  Google Scholar 

  • Seeliger, U. 2001. The Patos Lagoon Estuary, Brazil. In Coastal Marine Ecosystems of Latin America, Ecological Studies, vol. 144, ed. U. Seeliger and B. Kjerfve. Berlin: Springer.

    Chapter  Google Scholar 

  • Singh, N.P., M.T. McCoy, R.R. Tice, and E.L. Scheider. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research 237: 3–4.

    Google Scholar 

  • Sinha, R.P., and D.P. Hader. 2002. Life under solar uv radiation in aquatic organisms. Advances and Space Research 30(6): 1547–1556.

    Article  CAS  Google Scholar 

  • Sinha, R.P., M. Klisch, E.W. Helbling, and D.P. Hader. 2001. Induction of mycosporine-like amino acids (MAAs) in cyanobacteria by solar ultraviolet-B radiation. Journal of Photochemistry and Photobiology. B, Biology 60: 129–135.

    Article  CAS  Google Scholar 

  • Sinque, C., Muelbert, J.H. 1997. Ichthyoplankton. In: Selliger, U., C. Odebrecht and J.P. Castello. (org.). Subrtopical convergence environments: the coast and sea in the Southwestern Atlantic. Berlin: Springer-Verlag, p. 51–56.

  • Soares, I., and O.O. Möller. 2001. Low-frequency currents and water mass spatial distribution on the southern Brazilian shelf. Continental Shelf Research 21: 1785–1814.

    Article  Google Scholar 

  • Sommaruga, R., and G. Augustin. 2006. Seasonality in UV transparency of an alpine lake is associated to changes in phytoplankton biomass. Aquatic Sciences 68: 129–141.

    Article  Google Scholar 

  • Souza, M.S., B.E. Modenutti, and E.G. Balseiro. 2007. Antioxidant defences in planktonic crustaceans exposed to different underwater light irradiances in Andean Lakes. Water, Air, and Soil Pollution 183: 49–57.

    Article  CAS  Google Scholar 

  • Stambler, N., C. Lovengreen, and M.M. Tilzer. 1997. The underwater light field in the Bellingshausen and Amundsen Seas (Antarctica). Hydrobiologia 344: 41–56.

    Article  CAS  Google Scholar 

  • Stedmon, C.A., S.M. Arkager, and H.K. AAS. 2000. Optical properties and signatures of chromophoric dissolved organic matter (CDOM) in Danish coastal waters. Estuarine, Coastal and Shelf Science 51: 267–278.

    Article  CAS  Google Scholar 

  • Steinert, S.A., R. Streib-Montee, and J.M. Leather. 1998. DNA damage in mussels at sites in San Diego Bay. Mutation Research: Fundamental and Molecular Mechanisms of Mutagenesis 399(1): 65–85.

    Article  CAS  Google Scholar 

  • Sze, N.D., M.K.W. Ko, D.K. Weisenstein, J.M. Rodriguez, R.S. Stolarski, and M.R. Schoeberl. 1989. Antarctic ozone hole—Possible implications for ozone trends in the Southern-Hemisphere. Journal of Geophysical Research, [Atmospheres] 94(D9): 11521–11528.

    Article  CAS  Google Scholar 

  • Vehniäinen, E.R., J.M. Häkkinen, and A.O.J. Oikari. 2007. Responses to ultraviolet radiation in larval pike, Esox lucius, of two origins and ages. Boreal Environment Research 12: 673–680.

    Google Scholar 

  • Villafañe, V.E., P.J. Janknegt, M. Graaff, R.J.W. Visser, W.H.V. Poll, H. Willem, A.G.J. Buma, and E.W. Helbling. 2008. UVR-induced photoinhibition of summer marine phytoplankton communities from Patagônia. Marine Biology 154(6): 1021–1029.

    Article  Google Scholar 

  • Wake, D.B. 1991. Declining amphibian populations. Science 253: 860.

    Article  CAS  Google Scholar 

  • Weatherhead, E.C., and S.B. Andersen. 2006. The search for signs of recovery of the ozone layer. Nature 44: 39–45.

    Article  Google Scholar 

  • Welschmeyer, N.A. 1994. Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments. Limnology and Oceanography 39: 1985–1992.

    Article  CAS  Google Scholar 

  • Whitehead, R.F., S.J. de Mora, and S. Demers. 2000. Enhanced UV radiation—a new problem for the marine environment. In The effects of UV radiation in the marine environment, ed. S. De Mora, S. Demers, and M. Vernet, 1–34. Cambridge: New York.

    Chapter  Google Scholar 

  • Williamson, C.E., S.L. Metzgar, P.A. Lovera, and R.E. Moeller. 1997. Solar ultraviolet radiation and the spawning habitat of yellow perch, Perca flavescens. Ecological Applications 7: 1017–1023.

    Article  Google Scholar 

  • Youn, H.Y., V. Bantseev, N.C. Bols, A.P. Cullen, and J.G. Sivak. 2007. In vitro assays for evaluating the ultraviolet B-induced damage in cultured human retinal pigment epithelial cells. Journal of Photochemistry and Photobiology B: Biology 88: 21–28.

    Article  CAS  Google Scholar 

  • Zellmer, I.D. 1998. The effect of solar UVA and UVB on subarctic Daphnia pulicaria in its natural habitat. Hydrobiologia 379: 55–62.

    Article  Google Scholar 

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Acknowledgments

This study was financially supported by PELD (CNPq Grants 520188/98-5). This work was carried out with the aid of a grant from the Inter-American Institute for Global Change Research (IAI) CRN-2076 which is supported by the US National Science Foundation (Grant GEO-0452325). G.R.G. and M.R.S.G.L. were fellows from the Brazilian agencies CAPES and FAPERGS, respectively. J.H.M. benefited from a CNPq grant (Proc. 310931/2012-6). We are thankful to Marcela S. da Silveira for helping in the field work and to Dr. Paulo C. Abreu for the use of the radiometer/photometer. Author’s also acknowledge to Dra. Virginia Maria Tavano Garcia for comments and suggestions and to Dr. Robert Tew Boyle for revising the manuscript.

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  1. Instituto de Oceanografia—Programa de Pós-Graduação em Oceanografia Biológica, Universidade Federal do Rio Grande (FURG), Av. Itália Km 8, 96203-900, Rio Grande, RS, Brazil

    G. R. Gouveia & José Henrique Muelbert

  2. Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brazil

    G. R. Gouveia, G. S. Trindade & Luiz Eduardo Maia Nery

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  1. G. R. Gouveia
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  3. Luiz Eduardo Maia Nery
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Gouveia, G.R., Trindade, G.S., Nery, L.E.M. et al. UVA and UVB Penetration in the Water Column of a South West Atlantic Warm Temperate Estuary and its Effects on Cells and Fish Larvae. Estuaries and Coasts 38, 1147–1162 (2015). https://doi.org/10.1007/s12237-015-9996-5

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