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Effect of Zn toxicity on root morphology, ultrastructure, and the ability to accumulate Zn in Moso bamboo (Phyllostachys pubescens)

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Abstract

The effects of zinc (Zn) on seed germination and growth of Moso bamboo (Phyllostachys pubescens) were investigated. Under zinc stress, the seed germination rate did not show significant difference from that of the control. Hydroponics experiments indicated that Moso bamboo had a strong ability to accumulate Zn in the shoot and it reached its maximum value in the shoot at 100 μM Zn. The root Zn concentration ranged from 2,329.29 to 8,642.51 mg kg−1, with the root Zn concentration at 10 μM Zn being 58.23 times that of the control. The root morphology parameters slightly increased at the lower Zn treatments, while growth restriction was evident at higher Zn treatments. Root ultrastructural studies revealed that the cell structure, root tips, and organelles were significantly changed under Zn stress as compared to those of the control. Some abnormalities were evident in the cell walls, vacuoles, mitochondria, plasmalemma, tonoplast, and xylem parenchyma of root cells. While Moso bamboo seems a suitable candidate for phytoremediation, its metal remediation ability should be further explored in future investigations.

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Acknowledgments

The study was financially supported by a grant from the Natural Science Foundation of China (31300520), Zhejiang Province Natural Science Foundation of China (LY12C16004), and Science and Technology Program of Zhejiang Province (2014C33043).

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Authors and Affiliations

  1. Zhejiang Province Key Laboratory of Carbon Cycling in Forest Ecosystems and Carbon Sequestration, Zhejiang A&F University, Lin’an, 311300, Zhejiang, China

    Dan Liu, Junren Chen, Song Li, Jiasen Wu, Zhengqian Ye, Danli Peng, Wenbo Yan & Kouping Lu

  2. Department of Environmental Sciences, COMSATS Institute of Information Technology, Abbottabad, 22060, Pakistan

    Qaisar Mahmood

Authors
  1. Dan Liu
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  2. Junren Chen
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  3. Qaisar Mahmood
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  4. Song Li
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  5. Jiasen Wu
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  6. Zhengqian Ye
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  7. Danli Peng
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  8. Wenbo Yan
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  9. Kouping Lu
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Correspondence to Dan Liu.

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Responsible editor: Elena Maestri

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Liu, D., Chen, J., Mahmood, Q. et al. Effect of Zn toxicity on root morphology, ultrastructure, and the ability to accumulate Zn in Moso bamboo (Phyllostachys pubescens). Environ Sci Pollut Res 21, 13615–13624 (2014). https://doi.org/10.1007/s11356-014-3271-3

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  • DOI: https://doi.org/10.1007/s11356-014-3271-3

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