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Application of independent immobilization in benzo[a]pyrene biodegradation by synthetic microbial consortium

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Abstract

Bioaugmentation is an effective approach to remove the benzo[a]pyrene (BaP) from the environment, while its effect depends on the functional stability of the inoculated microorganisms. The aim of this study is to develop an approach on reducing the mutual exclusion of bacteria in the synthetic consortium in BaP degradation. Eight BaP-degrading bacterial strains were isolated from an enrichment with BaP as the sole carbon source. Two strains of Cupriavidus spp. exhibited greater degradation capacity (3.02–3.30 mg L−1 day−1) and selected as the “good degraders” in the synthetic consortia. Because of the mutual exclusion, the BaP-degradation capacity was reduced (1.47–1.77 mg L−1 day−1) when the other strains were added into “good degraders” through directly mixing the inocula. This mutual exclusion was mitigated through independent immobilization, in which the strains were embedded in sodium alginate before constructing the consortium. The consortium constructed by independent immobilization exhibited comparable BaP-degradation capacity with the high efficient strains. Therefore, the independent immobilization can be an advanced approach in functional consortium synthesis.

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Funding

This work was supported by grants from the National Natural Science Foundation of China (No. 51508111), the Natural Science Foundation of Guangdong Province, China (No. 2016A030313222), and Pearl River S&T Nova Program of Guangzhou, China (No. 201806010050).

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Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou, 510275, China

    Jiaming Huang, Qunhe Wu, Shunzhi Mai & Haochun Chi

  2. State Key Laboratory of Applied Microbiology Southern China, Guangdong Institute of Microbiology, Guangzhou, 510070, China

    Xunan Yang

  3. Guangdong Open Laboratory of Applied Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangzhou, 510070, China

    Xunan Yang

  4. Envitek E.P. (GUANGDONG) Inc., Guangzhou, 510507, China

    Xunan Yang

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  1. Jiaming Huang
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  2. Xunan Yang
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  4. Shunzhi Mai
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Correspondence to Xunan Yang.

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Huang, J., Yang, X., Wu, Q. et al. Application of independent immobilization in benzo[a]pyrene biodegradation by synthetic microbial consortium. Environ Sci Pollut Res 26, 21052–21058 (2019). https://doi.org/10.1007/s11356-019-05477-4

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  • DOI: https://doi.org/10.1007/s11356-019-05477-4

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