Abstract
Exposure to fine particulate matter (PM2.5) could induce lung impairment aggravation. Moreover, endogenous substances are known to play a significant role in lung impairment. Therefore, the research objectives was to investigate the influence of PM2.5-induced lung impairment on the levels of the eight endogenous substances, γ-aminobutyric acid (GABA), acetylcholine (ACh), glutamate (Glu), serotonin (5-HT), 5-hydroxyindole-3-acetic acid (5-HIAA), noradrenaline (NE), dopamine (DA), and 3, 4-dihydroxyphenylacetic acid (DOPAC). A sensitive UPLC-MS/MS method for the simultaneous determination of these endogenous substances in rat plasma and lung tissues was developed. The validated method was successfully applied for comparing profiles of analytes in rat plasma and lung tissues. The results indicated that five endogenous substances, namely, GABA, Ach, Glu, DA, and DOPAC, had a significant change in the rats with PM2.5-induced lung impairment.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bakowitz M, Bruns B, McCunn M (2012) Acute lung injury and the acute respiratory distress syndrome in the injured patient. Scandi J Trauma Resuscit Emer Med 20:54–65. https://doi.org/10.1186/1757-7241-20-54
Bourcier S, Benoist JF, Clerc F, Rigal O, Taghi M, Hoppilliard Y (2006) Detection of 28 neurotransmitters and related compounds in biological fluids by liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom 20:1405–1421. https://doi.org/10.1002/rcm.2459
Deng X, Zhang F, Rui W, Long F, Wang L, Feng Z, Chen D, Ding W (2013) PM2.5-induced oxidative stress triggers autophagy in human lung epithelial A549 cells. Toxicol in Vitro 27:1762–1770. https://doi.org/10.1016/j.tiv.2013.05.004
Dushianthan A, Grocott MPW, Postle AD (2011) Acute respiratory distress syndrome and acute lung injury. Postgrad Med J 87:612–622. https://doi.org/10.1136/pgmj.2011.118398
Eisner MD, Parsons P, Matthay MA (2003) Plasma surfactant protein levels and clinical outcomes in patients with acute lung injury. Thorax 58:983–988. https://doi.org/10.1136/thorax.58.11.983
Feng B, Song Y, Xu Q, Xu P, Zeng Q, Shan B, Liu K, Su D (2018) Simultaneous determination of savaside A, acteoside, and isoacteoside in rat plasma by UHPLC-MS/MS: comparative pharmacokinetic and bioavailability characteristics of Monochasma savatieri via different routes of administration. J Sep Sci 41(24):4408–4418. https://doi.org/10.1002/jssc.201800545
Guidance for Industry, Bioanalytical Method Validation (updated), 2001 http://www.fda.gov/cder/guidance/index.htm
Guideline on bioanalytical method validation (2011) http://www.ema.europa.eu/docs/en GB/document library/Scientific guideline/2011/08/WC500109686.pdf
Kim KH, Kabir E, Kabir S (2015) A review on the human health impact of airborne particulate matter. Environ Int 74:136–143. https://doi.org/10.1016/j.envint.2015.06.002
Kitaichi Y, Inoue T, Nakagawa S, Boku S, Kakuta A, Izumi T, Koyama T (2010) Sertraline increases extracellular levels not only of serotonin, but also of dopamine in the nucleus accumbens and striatum of rats. Eur J Pharmacol 647:90–96. https://doi.org/10.1016/j.ejphar.2010.08.026
Liu K, Song Y, Liu Y, Peng M, Li H, Li X, Feng B, Xu P, Su D (2017) An integrated strategy using UPLC-QTOF-MSE and UPLC-QTOF-MRM (enhanced target) for pharmacokinetics study of wine processed Schisandra Chinensis fructus in rats. J Pharm Biomed Anal 139:165–178. https://doi.org/10.1016/j.jpba.2017.02.043
Lodovici M, Bigagli E (2011) Oxidative stress and air pollution exposure. J Toxicol 487074:1–9. https://doi.org/10.1155/2011/487074
Longhin E, Holme JA, Gutzkow KB, Arlt VM, Kucab JE, Camatini M, Gualtieri M (2013) Cell cycle alterations induced by urban PM2.5 in bronchial epithelial cells: characterization of the process and possible mechanisms involved. Part Fibre Toxicol 10:63–75. https://doi.org/10.1186/1743-8977-10-63
Ma M, Li S, Jin H, Zhang Y, Xu J, Chen D, Kuimin C, Yuan Z, Xiao C (2015) Characteristics and oxidative stress on rats and traffic policemen of ambient fine particulate matter from Shenyang. Sci Total Environ 526:110–115. https://doi.org/10.1016/j.scitotenv.2015.04.075
Mazzoli-Rocha F (2010) Roles of oxidative stress in signaling and inflammation induced by particulate matter. Cell Biol Toxicol 26:481–498. https://doi.org/10.1007/s10565-010-9158-2
Mills NL, Donaldson K, Hadoke PW, Boon NA, MacNee W, Cassee FR, Sandstrom T, Blomberg A, Newby DE (2009) Adverse cardiovascular effects of air pollution. Nat Clin Pract Cardiovasc Med 6:36–44. https://doi.org/10.1038/ncpcardio1399
Nathan CR, Lang E, Dowling S (2011) Dopamine versus norepinephrine in the treatment of shock. CJEM 13:395–397. https://doi.org/10.2310/8000.2011.110297
Pun VC, Kazemiparkouhi F, Manjourides J, Suh HH (2017) Long-term PM2.5 exposure and respiratory, cancer, and cardiovascular mortality in older US adults. Am J Epidemiol 186(8):961–969. https://doi.org/10.1093/aje/kwx166
Ritz B, Liew Z, Yan Q, Cui X, Virk J, Ketzel M, Raaschou-Nielsen O (2018) Air pollution and Autism in Denmark. Environ Epidemiol 2(4):e028. https://doi.org/10.1097/EE9.0000000000000028
Samet JM, Rappold A, Graff D, Cascio WE, Berntsen JH, Huang YCT (2009) Concentrated ambient ultrafine particle exposure induces cardiac changes in young healthy volunteers. Am J Respir Crit Care Med 179:1034–1042. https://doi.org/10.1164/rccm.200807-1043OC
Tracey KJ (2007) Physiology and immunology of the cholinergic anti-inflammatory Pathway. J Clin Invest 117:289–296. https://doi.org/10.1172/JCI30555
Tryner J, Good N, Wilson A, Clark ML, Peel JL, Volckens J (2019) Variation in gravimetric correction factors for nephelometer-derived estimates of personal exposure to PM2.5. Environ Pollut 250:251–261. https://doi.org/10.1016/j.envpol.2019.03.121
Vardoulakis S, Fisher BEA, Pericleous K, Gonzalez-Fresca N (2003) Modelling air quality in street canyons, a review. Atmos Environ 37:155–182. https://doi.org/10.1016/s1352-2310(02)00857-9
Vattanasit U, Navasumrit P, Khadka MB, Kanitwithayanun J, Promvijit J, Autrup H, Ruchirawat M (2014) Oxidative DNA damage and inflammatory responses in cultured human cells and in humans exposed to traffic-related particles. Int J Hyg Environ Health 217:23–33. https://doi.org/10.1016/j.ijheh.2013.03.002
Wang G, Zhao J, Jiang R, Song W (2015) Rat lung response to ozone and fine particulate matter (PM2.5) exposures. Environ Toxicol 30:343–356. https://doi.org/10.1002/tox.21912
Wei BB, Liu MY, Chen ZX, Wei MJ (2018) Schisandrin ameliorates cognitive impairment and attenuates Aβ deposition in APP/PS1 transgenic mice: involvement of adjusting neurotransmitters and their metabolite changes in the brain. Acta Pharmacol Sin 39(4):616–625. https://doi.org/10.1038/aps.2017.135
Xia B, Zhou Y, Zhu Q, Zhao Y, Wang Y, Ge W, Yang Q, Zhao Y, Wang P, Si J, Luo R, Li J, Shi H, Zhang Y (2019) Personal exposure to PM2.5 constituents associated with gestational blood pressure and endothelial dysfunction. Environ Pollut 250:346–356. https://doi.org/10.1016/j.envpol.2019.04.024
Zhao H, Yang B, Xu J, Chen DM, Xiao CL (2017) PM 2.5-induced alterations of cell cycle associated gene expression in lung cancer cells and rat lung tissues. Environ Toxicol Pharmacol 52:77–82. https://doi.org/10.1016/j.etap.2017.03.014
Acknowledgements
We thank the support by the Shenyang Scientific Project (No. 17-231-1-45), Shenyang Medical College Scientific Project (No. 20163042), and Shenyang Medical College student scientific research Project (No. 20179034).
Funding
This study was supported by Liaoning Province Natural Scientific Project (No. 20180550122), Shenyang Major Technology Research and Development Project (No. 18-400-4-09), and Liaoning Province Science and Technology Project (No. 2017225076).
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The experimental protocol was approved by the Animal Ethics Committee of Shenyang Medical College (Permit number: 2016-0151), and all animal studies were carried out in accordance with the Guidelines for Animal Experimentation.
Competing interests
The authors declare that they have no competing interests.
Additional information
Responsible editor: Philippe Garrigues
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Electronic supplementary material
ESM 1
(XLS 281 kb)
Rights and permissions
About this article
Cite this article
Fan, R., Ren, Q., Zhou, T. et al. Determination of endogenous substance change in PM2.5-induced rat plasma and lung samples by UPLC-MS/MS method to identify potential markers for lung impairment. Environ Sci Pollut Res 26, 22040–22050 (2019). https://doi.org/10.1007/s11356-019-05351-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11356-019-05351-3