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Effects of low glyphosate-based herbicide concentrations on endocrine-related gene expression in the decapoda Macrobrachium potiuna

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Abstract

Glyphosate-based herbicides (GBH) are the most used herbicides worldwide and are considered as endocrine-disrupting compounds (EDC) for non-target organisms. However, effects of GBH on their endocrine systems remain poorly understood. Thus, the aim of this study was to assess the effects of low concentrations of Roundup WG® on growth and reproduction process molecules in both males and females of the decapod crustacean Macrobrachium potiuna, by the relative transcript expression levels of the ecdysteroid receptor (EcR), the molt-inhibiting hormone (MIH), and the vitellogenin (Vg) genes. Prawns were exposed to three concentrations of GBH (0.0065, 0.065, and 0.28 mg L−1) for 7 and 14 days. The results revealed that only in males the three genes transcript levels were influenced by the GBH concentration, time of exposure, and the interaction between the concentrations and time of exposure, suggesting that males were more sensitive to GBH than females. For males, after 7 days of exposure at 0.065 mg L−1, EcR and MIH were over-expressed, while the Vg expression was only over-expressed after 14 days. The present study highlighted that GBH impacted endocrine systems of M. potiuna. Moreover, EcR and MIH gene expressions could be promising EDC biomarkers of exposure in crustaceans. These results also indicate that GBH concentrations, considered secure by regulatory agencies, should be reviewed to minimize the effects on non-target organisms.

Potential effects of glyphosate-based herbicides on the endocrine system of decapods Macrobrachium sp.

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Funding

This work was conducted during a scholarship supported by the Programa de Doutorado Sanduíche no Exterior from the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES/PDSE 47/2017) at the University of Liège.

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  1. Departamento de Biologia Celular, Embriologia e Genética, Universidade Federal de Santa Catarina, Florianopolis, Santa Catarina, Brazil

    Madson Silveira de Melo, Evelise Maria Nazari & Yara Maria Rauh Muller

  2. Laboratory of Animal Ecology and Ecotoxicology (LEAE) - Chemistry Institute, University of Liège, Bât. B6C, 11 allée du 6 Août, Sart-Tilman, 4000, Liege, Belgium

    Célia Joaquim-Justo & Eric Gismondi

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  1. Madson Silveira de Melo
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  2. Evelise Maria Nazari
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  3. Célia Joaquim-Justo
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  4. Yara Maria Rauh Muller
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  5. Eric Gismondi
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Correspondence to Eric Gismondi.

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The sampling procedure adopted in this study was approved by the Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA; permanent license no. 15294-1/2008).

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Highlights

• GBH was able to upregulate the transcript expression levels of EcR and MIH.

• Males of Macrobrachium potiuna were more affected by the GBH exposure than females.

• Integrated biomarker response (IBR) shows an induction of biomarkers for males.

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de Melo, M.S., Nazari, E.M., Joaquim-Justo, C. et al. Effects of low glyphosate-based herbicide concentrations on endocrine-related gene expression in the decapoda Macrobrachium potiuna. Environ Sci Pollut Res 26, 21535–21545 (2019). https://doi.org/10.1007/s11356-019-05496-1

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