Abstract
Background, aim, and scope
Due to the active production and transport of crude oil in the Arabian Gulf region, the Arabian Gulf coasts are routinely polluted with oil. Therefore, such coasts have been subject of studies aiming at assessing the roles of indigenous microbial consortia in cleaning these environments. In the present study, epilithic microbial communities along Kuwait coasts were studied for their oil degradation potential.
Materials and methods
Gravel particles coated with deep green biofilms were collected from four coastal sites in autumn, winter, and spring. Phototrophs in these consortia were determined in terms of their chlorophyll a contents and identified by their morphological characteristics. Total bacteria were counted microscopically and cultivable bacteria by the dilution plating method on nutrient agar as well as on inorganic medium containing oil as a sole source of carbon and energy. The bacterial community structures were also characterized and compared by denaturing gradient gel electrophoresis (DGGE).
Results
Epilithic biomass samples from the four sites in the three seasons were rich in diatoms and picocyanobacteria as well as total bacteria. Direct counting gave bacterial numbers per square centimeter gravel surface of 2 to 6 × 107 cells depending on the sampling site and season. Cultivable bacterial numbers on nutrient agar and crude oil as a sole source of carbon were 3 × 103 to 8 × 104 and 1 × 103 to 7 × 103 cells/cm2 gravel surface, respectively. The DGGE profiles of epilithon biomass samples revealed major 16S rDNA bands that matched bands of pure oil-utilizing bacterial isolates.
Discussion
The microbial communities showed a degree of consistency in all sites and seasons.
Conclusions
The microbial consortia coating gravel particles are potentially suitable tools for self-cleaning of oily Gulf coasts. They are rich in oil-utilizing bacteria whose activities are probably enhanced by oxygen produced by the phototrophic partners in the consortia.
Recommendations and perspectives
The combination of conventional microbiological analysis with molecular approaches gives an enhanced idea about natural microbial communities especially those with environmental application potential.
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References
Ainsworth AM, Goulder R (1998) Microbial organic-nitrogen transformations along the Swale-Ouse river system, Northern England. Sci Total Environ 210(211):329–355
Al-Awadhi H, Al-Hasan RH, Sorkhoh NA, Salamah S, Radwan SS (2003) Establishing oil-degrading biofilms on gravel particles and glass plates. Int Biodeter Biodeg 51:181–185
Antic MP, Jovancicevic B, Ilic M, Vrvic MM, Schwarzbouer J (2006) Petroleum pollutant degradation by surface water microorganisms. Environ Sci Pollut Res 13:320–327
Bernard L, Schafer H, Joux F, Courties C, Muyzer G, Lebaron P (2000) Genetic diversity of total, active and culturable marine bacteria in coastal seawater. Aquat Microb Ecol 22:1–11
British Petroleum Co (1979) BP Statistical Review of the World Oil Industry. British Petroleum, London
Chappell KR (1994) Extracellular Enzyme Activity in Freshwaters. PhD thesis. UK: University of Hull
El Samra MI, Emara HI, Shunbo F (1986) Dissolved petroleum hydrocarbons in the Northwestern Arabian Gulf. Mar Pollut Bull 17:65–68
Hunter JR (1982) The physical oceanography of the Arabian Gulf: a review and theoretical interpretation of previous observations. In: Halwagy R, Clayton D, Behbehani M (eds) The First Arabian Gulf Conference on Environment and Pollution. Kuwait University, Faculty of Science, Kuwait, pp 1–23
Isikhuemhen O, Anollefo G, Oghale O (2003) Bioremedaition of crude oil polluted soil by the white rot fungus, Pleurotus tuberregium (Fr.) Sing. Environ Sci Pollut Res 10:108–112
Jesperson AM, Christoffersen K (1987) Measurements of chlorophyll a from phytoplankton using ethanol as extraction solvent. Arch Hydrobiol 109:445–454
Jones R, Sun W, Tang C-S, Robert F (2004) Phytoremediation of petroleum hydrocarbons in tropical coastal soils–II–microbial response to plant roots and contaminants. Environ Sci Pollut Res 11:340–346
Kuske CR, Banton KL, Adorada DL, Strak PC, Hill KK, Jackson PJ (1998) Small scale DNA sample preparation method for field PCR detection of microbial cells and spores in soil. Appl Environ Microbiol 64:2463–2472
Marchand M, Monfort JP, Rubio AC (1982) Distribution of hydrocarbons in water and marine sediments after the Amoco Cadez and Istoc. 1 oil spills. In: Keith L (ed) Energy and Environmental Chemistry. Arbor Science, MI, vol 1, pp 487–509
Muyzer G, Smalla K (1998) Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Anton Leeuw Int J 73:127–141
Pu X, Cutright T (2007) Degradation of pentachlorophenol by pure and mixed cultures in two different soils. Environ Sci Pollut Res 14:244–250
Radwan S (2008) Microbiology of oil-contaminated desert soils and coastal areas in the Arabian Gulf region. In: Dion P, Nautiyal CS (eds) Microbiology of Extreme Soils, Soil Biology 13. Springer, Berlin, pp 275–298
Radwan SS, Al-Hasan RH (2001) Potential application of coastal biofilm-coated gravel particles for treating oily waste. Aqu Microb Ecol 23:113–117
Radwan SS, Al-Hasan RH, Al-Awadhi H, Salamah S, Abdullah HM (1999) Higher oil biodegradation potential at the Arabian Gulf coast than in the water body. Mar Biol 135:741–745
Radwan SS, Al-Hasan RH, Mahmoud HM, Eliyas M (2007) Oil Utilizing bacteria associated with fish from the Arabian Gulf. J Appl Microbiol 103:2160–2167
Radwan SS, Al-Hasan RH, Salamah S, Al-Dabbous S (2002) Bioremediation of oily sea water by bacteria immobilized in biofilms coating macroalgae. Int Biodeter Biodeg 50:55–59
Radwan SS, Al-Hasan RH, Salamah S, Khanafer M (2005) Oil-consuming microbial consortia floating in the Arabian Gulf. Int Biodeter Biodeg 56:28–33
Rehm H-J, Reiff I (1981) Mechanisms and occurrence of microbial oxidation of long-chain alkanes. Adv Biochem Eng 19:175–216
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York
Santegoeds CM, Ferdelman TG, Muyzer G, Beer DD (1998) Structural and functional dynamics of sulfate-reducing populations in bacterial biofilms. Appl Environ Microbiol 64:3731–3739
Sen Gupta R, Kureishy TW (1981) Present stage of oil pollution in the northern Indian Ocean. Mar Pollut Bull 12:295–301
Singer ME, Finnerty WR (1984) Microbial metabolism of straight-chain and branched alkanes. In: Atlas RM (ed) Petroleum Microbiology. Macmillan, New York, pp 1–59
Sorkhoh N, Al-Hasan RH, Radwan S, Höpner T (1992) Self-cleaning of the Gulf. Nature 359:109
Sorkhoh NA, Ghannoum MA, Ibrahim AS, Stretton RJ, Radwan SS (1990) Crude oil and hydrocarbon degrading strains of Rhodococcus rhodochrous isolated from soil and marine environments in Kuwait. Environ Pollut 65:1–17
Teske A, Wawer C, Muyzer G, Ramsing NB (1996) Distribution of sulfate-reducing bacteria in a stratified Fjord (Maiager Fjord, Denmark) as evaluated by most-probable-number counts and denaturing gradient gel electrophoresis of PCR-amplified ribosomal DNA fragments. Appl Environ Microbiol 62:1405–1415
Wawer C, Muyzer G (1995) Genetic diversity of Desulfovibrio spp. in environmental samples analysed by denaturing gradient gel electrophoresis of [NiFe] hydrogenase gene fragments. Appl Environ Microbiol 62:2203–2210
Yu W, Dodds WK, Banks K, Skalsky J, Staruss E (1995) Optimal staining and sample storage time for direct microscopic enumeration of total and active bacteria in soil with two fluorescent dyes. Appl Environ Microbiol 61:3367–3372
Acknowledgments
This work has been supported by the Kuwait University, Research Grant RS 02/04. Thanks are due to Amar Habib for technical assistance.
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Mahmoud, H., Al-Hasan, R., Khanafer, M. et al. A microbiological study of the self-cleaning potential of oily Arabian Gulf coasts. Environ Sci Pollut Res 17, 383–391 (2010). https://doi.org/10.1007/s11356-009-0099-3
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DOI: https://doi.org/10.1007/s11356-009-0099-3