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Reproductive functions of wild fish as bioindicators of reproductive toxicants in the aquatic environment

  • AREA 7.2 • IMPACTS OF CHEMICALS / FATE IN BIOTA • RESEARCH ARTICLE
  • Published:
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Abstract

Background, aim, and scope

Impacts on the reproductive health of wild fish are thought to be suitable early-warning tools indicating contamination of surface waters with endocrine-disrupting compounds. Ecotoxicological assessment of these field observations depends on the availability of reliable biomarkers to enable a discrimination of natural variations of reproductive functions from anthropogenic impacts.

Materials and methods

Roach and perch were caught at eight sampling sites by electrofishing twice a year in summer (July–September) and late autumn/winter (November–December) over a 2-year period. The sites are characterized by different degrees of anthropogenic impact and are situated within the greater Upper Rhine catchment. Age growths, parasitization and gonadal histology of more than 3,000 fish were examined.

Results

The two dominant fish species in German surface waters perch (Perca fluviatilis L.) and roach (Rutilus rutilus L.) differ considerably regarding their suitability for biomonitoring. Even in pristine habitats, perch show several variants of sex differentiation in terms of (1) the time of first sexual maturation, (2) the course of seasonal gonadal recrudescence, and (3) the occurrence of heterologous germ cells (testes ova). A statistically significant elevated proportion of males were observed in fish obtained from a TBT-contaminated marina and suppression of gonadal ripening was observed in females caught in a sewage-contaminated brook. Both effects appear to be due to chemical contamination. The only “natural” alteration of sex differentiation in roach was related to parasitization with Ligula intestinalis (Eucestoda, Pseudophyllidea). Other deviations from the normal pattern of sex differentiation were (1) suppression of ovarian ripening and (2) asynchronic seasonal gonadal recrudescence. These are strong indicators of an anthropogenically induced impact on reproductive health. Feminization phenomena were not observed at either the individual or the population level.

Discussion

Interpretation of field monitoring results concerning reproductive health requires large numbers of samples and detailed knowledge of the natural plasticity of sex differentiation in the species under investigation. A better understanding of the mechanisms underlying the plasticity of sex differentiation in perch is indispensable to enable perch to be used as a bioindicator.

Conclusions

Deviation from the strict and probably endogenous control of sex differentiation in roach is a strong and unequivocal warning signal.

Recommendations and perspectives

The subject of fish monitoring should be addressed in the context of a broader spectrum of potential risks. Seasonal and ontogenetic integrity of gonadal development and recrudescence are potent biomarkers, provided the natural process is well documented for the species under investigation.

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Acknowledgements

The study was supported by the German Association of the Chemical Industry (VCI), Project ID 2000/00954.

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Authors and Affiliations

  1. GOBIO GmbH, Scheidertalstr. 69a, 65326, Aarbergen, Germany

    Bernhard Allner, Sabine von der Gönna, Nadja Nikutowski, Annette Weltin & Petra Stahlschmidt-Allner

  2. Department of Ecology, Zoological Institute, University of Mainz, P.O. Box 3980, 55099, Mainz, Germany

    Eva-Maria Griebeler

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  1. Bernhard Allner
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  2. Sabine von der Gönna
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  3. Eva-Maria Griebeler
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  4. Nadja Nikutowski
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  5. Annette Weltin
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  6. Petra Stahlschmidt-Allner
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Correspondence to Petra Stahlschmidt-Allner.

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Responsible editor: Thomas Braunbeck

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Allner, B., von der Gönna, S., Griebeler, EM. et al. Reproductive functions of wild fish as bioindicators of reproductive toxicants in the aquatic environment. Environ Sci Pollut Res 17, 505–518 (2010). https://doi.org/10.1007/s11356-009-0149-x

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