Summary
Cell division during embryonic development of the brine shrimp,Artemia salina has been studied by counting nuclei and mitotic figures. No cell division was observed during development of the encysted gastrula until about an hour before emergence of the embryo (a pre-nauplius) from the cyst, and even then only a few mitotic figures were observed. Following emergence, and during further development up to the stage II nauplius larva an increase of about 25% in the number of cells occurs. However, when the newly hatched larva is exposed to FUdR (10 μg/ml) cell division is largely inhibited, but observable development nevertheless proceeds normally. Evidently all processes involved with the development of the gastrula into a stage II nauplius larva can occur with far fewer cells than normally are present.
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References
Avanzi, S., Brunori, A., D'Amato, F.: Sequential development of meristems in the embryo ofTriticum durum. A DNA autoradiographic and cytophotometric analysis. Develop. Biol.20, 368–377 (1969)
Avanzi, S., Brunori, A., D'Amato, F., Nuto Ronchi, V., Mugnozza, S.: Occurrence of 2C (G1) and 4C (G2) nuclei in the radicle meristems of dry seeds inTriticum durum Desf. Its implications in studies on chromosome breakage and on developmental processes. Caryologia16, 553–558 (1963)
Avanzi, S., Brunori, A., Georgi, B.: Radiation response of dry seed in two varieties ofTriticum durum. Mutation Res.3, 426–437 (1966)
Bellini, L.: Osservazioni sugli acidi nucleici nello sviluppo diArtemia salina Leach. Ric. Sci.30, 816–822 (1960)
Benesch, R.: Zur ontogenie und morphologie vonArtemia salina L. Zool. Jb. (Anat. Ontog. Tiere),86, 307–458 (1969)
Burton, K.: A study of the conditions and mechanisms of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem. J.62, 315–323 (1965)
Clegg, J.S.: The control of emergence and metabolism by external osmotic pressure and the role of free glycerol in developing cysts ofArtemia salina. J. Exp. Biol.41, 879–892 (1964)
Clegg, J.S.: Biochemical adaptations associated with the embryonic dormancy ofArtemia salina. Trans. Am. Microscop. Soc.93, 481–490 (1974)
Davidson, D.: The onset of mitosis and DNA synthesis in roots of germinating beans. Am. J. Bot.53, 491–495 (1966)
de Chaffoy, D., Kondo, M.: Selective resistance to desiccation of nuclear ribonucleic acid synthesis in isolated nuclei ofArtemia salina embryos during pre-emergence development. Biochem J.158, 643–646 (1976)
DeRobertis, E.D.P., Nowinski, W.W., Saez, F.A.: Cell Biology. Philadelphia and London: W.B. Saunders Co. 1975
Finamore, F.J., Clegg, J.S.: Biochemical aspects of morphogenesis in the brine shrimp,Artemia salina. In: The Cell Cycle (G.M. Padilla et al., eds.). New York and London: Academic Press 1969
Gelfant, S.: Initiation of mitosis in relation to the cell division cycle. Exp. Cell. Res.26, 395–403 (1962)
Graham, C.F., Morgan, R.W.: Changes in the cell cycle during early amphibian development. Develop. Biol.14, 439–450 (1966)
Grosfeld, H., Littauer, U.Z.: The translation in vitro of mRNA from developing cysts ofArtemia salina. Eur. J. Biochem.70, 589–599 (1976)
Heidelberger, C., Chanduri, W.K., Kanneberg, P., Mooren, D., Greisbach, L., Duschinski, B., Schnitzer, R.J., Pleven, E., Shreiner, J.: Fluorinated pyrimidines, a new class of tumorinhibitory compounds. Nature (London)179, 663 (1957)
Hentschel, C.C., Tata, J.R.: The molecular embryology of the brine shrimp. Trends in Biochemical Science1, 97–100 (1976)
Hyman, L.: The Invertebrates, Vol. III. Acanthocephala, Aschelminthes, and Entoprocta. New York: McGraw-Hill 1951
McClean, D.K., Warner, A.H.: Aspects of nucleic acid metabolism during the development of the brine shrimp,Artemia salina. Develop. Biol.24, 88–105 (1971)
Moens, L., Kondo, M.: Polysome-dependant synthesis of embryonic proteins ofArtemia salina during cell differentation and analysis of heme-containing protein. Develop. Biol.49, 457–469 (1976)
Morris, J.E.: Hydration, its reversibility, and the beginning of development in the brine shrimp,Artemia salina. Comp. Biochem, Physiol.39A, 843–857 (1971)
Muramatsu, S.: Studies on the physiology ofArtemia embryos. I. Respiration and its main substrate during early development. Embryologia5, 95–106 (1960)
Nakanishi, Y.H., Iwasaki, T., Okigaki, T., Kato, H.: Cytological studies ofArtemia salina. I. Embryonic development without cell multiplication after the blastula stage in encysted dry eggs. Annot. Zool. Japon.35, 223–228 (1962)
Nakanishi, T.H., Okigaki, T., Kato, H., Iwasaki, T.: Cytological studies ofArtemia salina. II. Deoxyribonucleic acid (DNA) content and the chromosomes in encysted dry eggs and nauplii. Annot. Zool. Japon,39, 306–309 (1963)
Nicholas, W.L.: The biology of free-living nematodes. London: Oxford University Press, 1975
Olson, C.S.: DNA synthesis and cell division during the development ofArtemia salina Leach. Doctoral Dissertation, Florida: University of Miami, Coral Gables, 1970
Sierra, J.M., Filipowicz, W., Ochoa, S.: mRNA in undeveloped and developingArtemia salina embryos. Biochem. Biophys. Res. Comm.69, 181–189 (1976)
Sin, W.C., Pasternak, J.: Number and DNA content of nuclei inPanagrellus silusiae. Chromosoma32, 191–204 (1970)
Sorgeloos, P.: A bibliography on the brine shrimp,Artemia salina. Special Publication No. 1 of the European Mariculture Society (E. Jaspers, ed.) Bredene, Belgium 1976
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Olson, C.S., Clegg, J.S. Cell division during the development ofArtemia salina . Wilhelm Roux' Archiv 184, 1–13 (1978). https://doi.org/10.1007/BF00848665
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DOI: https://doi.org/10.1007/BF00848665