Abstract
The decline of European abalone Haliotis tuberculata populations has been associated with various pathogens including bacteria of the genus Vibrio. Following the summer mortality outbreaks reported in France between 1998 and 2000, Vibrio harveyi strains were isolated from moribund abalones, allowing in vivo and in vitro studies on the interactions between abalone H. tuberculata and V. harveyi. This work reports the development of primary cell cultures from abalone gill tissue, a target tissue for bacterial colonisation, and their use for in vitro study of host cell—V. harveyi interactions. Gill cells originated from four-day-old explant primary cultures were successfully sub-cultured in multi-well plates and maintained in vitro for up to 24 days. Cytological parameters, cell morphology and viability were monitored over time using flow cytometry analysis and semi-quantitative assay (XTT). Then, gill cell cultures were used to investigate in vitro the interactions with V. harveyi. The effects of two bacterial strains were evaluated on gill cells: a pathogenic bacterial strain ORM4 which is responsible for abalone mortalities and LMG7890 which is a non-pathogenic strain. Cellular responses of gill cells exposed to increasing concentrations of bacteria were evaluated by measuring mitochondrial activity (XTT assay) and phenoloxidase activity, an enzyme which is strongly involved in immune response. The ability of gill cells to phagocyte GFP-tagged V. harveyi was evaluated by flow cytometry and gill cells-V. harveyi interactions were characterized using fluorescence microscopy and transmission electron microscopy. During phagocytosis process we evidenced that V. harveyi bacteria induced significant changes in gill cells metabolism and immune response. Together, the results showed that primary cell cultures from abalone gills are suitable for in vitro study of host-pathogen interactions, providing complementary assays to in vivo experiments.
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Acknowledgments
This work was supported by ECC (SUDEVAB N°222156 “Sustainable development of European SMEs engaged in abalone aquaculture”) and the Brittany region (Programme “Ormeaux”, Pôle Mer Bretagne). We thank Marion Cardinaud for the construction of the strain LMG7890-GFP tagged that she kindly provided for in vitro biotests on gill cells.
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Pichon, D., Cudennec, B., Huchette, S. et al. Characterization of abalone Haliotis tuberculata–Vibrio harveyi interactions in gill primary cultures. Cytotechnology 65, 759–772 (2013). https://doi.org/10.1007/s10616-013-9583-1
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DOI: https://doi.org/10.1007/s10616-013-9583-1