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Inorganic phosphate transport inAcinetobacter lwoffi

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Abstract

The transport of inorganic phosphate has been studied inAcinetobacter lwoffi JW11. During growth on excess phosphate, only one transport system was present, with an apparent Km of 1.4 μM. When cells were starved for phosphate, a second uptake system with an apparent Km of 110 nM was also synthesized. The two transport systems could be distinguished by differing sensitivities to the phosphate analogs arsenate and 2-aminoethylphosphonate. Both systems were inhibited by carbonylcyanidem-chlorophenylhydrazone, and to a lesser extent by Na azide. The high-affinity transport system was inactivated by osmotic shock treatment and by spheroplast formation. Preliminary evidence for a phosphate-binding protein in the osmotic shock fluid is presented. The isolation of a mutant constitutive for the high-affinity transport system is described.

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  1. Jacob Yashphe

    Present address: Department of Bacteriology, The Hebrew University, Hadassah Medical School, Jerusalem, Israel

Authors and Affiliations

  1. Marine Biological Laboratory, 02543, Woods Hole, Massachusetts, USA

    Jacob Yashphe, Hemant Chikarmane, Maria Iranzo &  Harlyn O. Halvorson

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  1. Jacob Yashphe
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  2. Hemant Chikarmane
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  3. Maria Iranzo
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  4. Harlyn O. Halvorson
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Yashphe, J., Chikarmane, H., Iranzo, M. et al. Inorganic phosphate transport inAcinetobacter lwoffi . Current Microbiology 24, 275–280 (1992). https://doi.org/10.1007/BF01577332

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