Abstract
Genetic variation and gene flow in the scleractinian coral Goniastrea aspera (Verrill), found around the north–south Okinawa Islands, were studied using allozyme and starch gel electrophoresis. The relative contribution of sexual and asexual reproduction to recruitment was determined. Analysis of multilocus genotypes of samples, collected at least 3 m apart, identified a high number of unique genotypes (NG) relative to the number of individuals sampled (N) (mean NG: N=0.97±0.03 [SD]), and also highly observed genotypic diversity (GO) relative to expected genotypic diversity under Hardy–Weinberg equilibrium (GE) (mean GO: GE=0.95±0.06 [SD]). These results suggest that the collected G. aspera propagated predominantly by sexual reproduction in Okinawan populations. The UPGMA grouping of five populations in the Okinawa, based on Nei’s unbiased genetic distance, showed two clusters that were south and north Okinawa populations. AMOVA analyses that incorporated the data of populations from the Kerama and the Ishigaki Islands detected significant FSC values among the populations within groups in analyses distinguishing north–south Okinawa, Okinawa–Kerama, and Okinawa–Kerama–Ishigaki. Significant FCT values were neither detected between north–south Okinawa nor between the Okinawa–Kerama groups. The “local-recruits” hypothesis, which assumes that a substantial proportion of recruits is produced locally, appears to be applicable to coral populations in the Ryukyu Archipelago. On the other hand, detection of nonsignificant FCT values indicated the presence of genetic connectivity between north–south Okinawa and Kerama–Okinawa groups.
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References
Abe N (1937) Postlarval development of the coral Fungia actiniformis var palawensis Döderlein. Palao Trop Biol Stat Stud 1:73–93
Adjeroud M, Tsuchiya M (1999) Genetic variation and clonal structure in the scleractinian coral Pocillopora damicornis in the Ryukyu Archipelago, southern Japan. Mar Biol 134:753–760
Ayre DJ, Hughes TP (2000) Genotypic diversity and gene flow in brooding and spawning corals along the Great Barrier Reef, Australia. Evolution 54(5):1590–1605
Ayre DJ, Willis BL (1988) Population structure in the coral Pavona cactus: clonal genotypes show little phenotypic plasticity. Mar Biol 99:495–505
Babcock RC (1984) Reproduction and distribution of two species of Goniastrea (Scleractinia) from the Great Barrier Reef province. Coral Reefs 2:187–204
Brown BE (1997) Coral bleaching: causes and consequences. Coral reefs 16:129–138
Dai CF, Fan TY, Yu JK (2000) Reproductive isolation and genetic differentiation of a scleractinian coral Mycedium elephantotus. Mar Ecol Prog Ser 201:179–187
Exocoffier L, Smouse P, Quattro J (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:479–491
Glynn PW (1991) Coral reef bleaching in the 1980s and possible connections with global warning. Trends Ecol Evol 6:175–179
Goreau TJ, McClanahan TR, Hayes RL, Strong A (2000) Conservation of coral reefs after the 1998 global bleaching event. Conserv Biol 14:5–15
Hillis DM, Moritz C, Barbara KM (1996) Molecular Systematics. Sinauer Associates, incorporated
Hoegh-Guldberg O (1999) Coral bleaching, climate change, and the future of the world’s coral reefs. J Mar Fresh Res 50:839–866
Loya Y, Sakai K, Yamazato K, Nakano Y, Sambali H, Van Woesik R (2001) Coral bleaching: the winners and losers. Ecol Lett 4:122–131
Miller MP (1997) Tools for population genetic analysis (TFPGA) 1.3: a Windows program for the analysis of allozyme and molecular population genetic data. Computer software distributed by the author
Ministry of the Environment of Japan (2002) Report of the international Coral Reef Research and Monitoring Center (in Japanese). International Coral Reef Research and Monitoring Center, Ishigaki, pp 13–34
Motoda S (1939) Observation of period of emergence of planulae of Goniastrea aspera Verrill (in Japanese, title translated by KS). Kagaku Nanyo 1:113–115
Nadaoka K, Harii S, Mitsui J, Tamura H, Hanada G, Paringit E, Nihei Y, Fujii S, Sato K, Matsuoka T, Kakuma S, Ikema T, Iwao K, and Takahashi T (2002a) Larval tracking using small drifters and larval settlement experiments to examine long-distance larval transport of corals (in Japanese). Proc Coastal Eng JSCE 49:366–370
Nadaoka K, Harii S, Ikema T, Paringit E, Mitsui J, Tamura H, Iwao K, Kakuma S (2002b) Coral Spawning and Slick Observations in Kerama Island, Okinawa (in Japanese). Proc Coastal Eng JSCE 49:1176–1180
Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–190
Nishikawa A, Sakai K (2003) Genetic variation and gene flow of broadcast spawning and planula brooding coral, Goniastrea aspera (Scleractinia) in the Ryukyu Archipelago, southern Japan. Zool Sci 20:1031–1038
Nishikawa A, Katoh M, Sakai K (2003) Larval settlement rates and gene flow of broadcast-spawning (Acropora tenuis) and planula-brooding (Stylophora pistillata) corals (Scleractinia). Mar Ecol Prog Ser 256:87–97
Sakai K (1997) Gametogenesis, spawning, and planula brooding by the reef coral Goniastrea aspera (Scleractinia) in Okinawa, Japan. Mar Ecol Prog Ser 151:67–72
Selander RK, Smith MH, Yank SY, Johnston WE, Gentry JR (1971) Biochemical polymorphism and systematics in the genus Peromyscus I Variation in the old-field mouse (Peromyscus polionotus). Stud Genet VI University of Texas Pub 7103:49–90
Stoddart JA (1983) Asexual production of planulae in the coral Pocillopora damicornis. Mar Biol 76:279–284
Stoddart JA (1984) Genetic structure within populations of the coral Pocillopora damicornis. Mar Biol 81:19–30
Stoddart JA, Taylor JF (1988) Genotypic diversity: estimation and prediction in samples. Genetics 118:705–711
Taniguchi H, Iwao K (2000) Change of coral number and density in Aka Island (In Japanese). Rep Akajima Mar Sci Lab 11:22–23
Wright S (1969) The evolution and genetics of populations. University of Chicago press, Chicago
Acknowledgements
We are indebted to the staff of Sesoko Station, Tropical Biosphere Research Center, University of the Ryukyus. Thanks are due to many students in TBRC for their encouragement, help, and advice. We are very grateful for two anonymous reviewers who helped improve the manuscript. This study was supported by Grant-in-Aid of Scientific Research (B) (No. 14205071) to K. Nadaoka and (C) (No. 12640619) to K. Sakai from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
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Nishikawa, A., Sakai, K. Genetic connectivity of the scleractinian coral Goniastrea aspera around the Okinawa Islands. Coral Reefs 24, 318–323 (2005). https://doi.org/10.1007/s00338-005-0484-4
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DOI: https://doi.org/10.1007/s00338-005-0484-4