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Determination of Celecoxib, Rofecoxib, Sodium Diclofenac and Niflumic Acid in Human Serum Samples by HPLC with DAD Detection

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Abstract

A reverse-phase high-performance liquid chromatographic method has been developed for the separation and simultaneous determination of two COX-2 inhibitors, celecoxib and rofecoxib, in addition to two well-known non-steroidal anti-inflammatory drugs (NSAIDs), sodium diclofenac and niflumic acid in human serum samples. Good chromatographic separation was achieved using a C18 bonded silica column applying a gradient with acetronitrile and water, from 15 to 60% acetonitrile. The mobile phase contained 0.1% trifluoroacetic acid as an organic modifier. Detection was made using a diode array detector (DAD) and the analytical parameters were established at the wavelength maximum in the UV spectrum of each drug. Linearity was studied up to 100.0 mg L−1. Calibration functions, quantification and detection limits, intra- and inter-day reproducibility and accuracy were estimated for each drug. Solid phase extraction was needed to separate and concentrate the drugs from human serum samples. The method was successfully applied to determine the drugs in human serum samples at levels of 1.0 mg L−1.

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References

  1. Lewis PJ, Dollery CT (1983) Br Med Bull 39:281–284

    CAS  Google Scholar 

  2. Samad TA, Sapirstien A, Woolf CJ (2002) Trends Mol Med 8:390–396

    Article  CAS  Google Scholar 

  3. Garavito RM, Malkowski MG, DeWitt DL (2002) Prostaglandins Others Lipid Mediat 129:68–69

    Google Scholar 

  4. Smith WL, Garavito RM, Dewitt DL (1996) J Biol Chem 271:33157–33160

    Article  CAS  Google Scholar 

  5. Bombardier C, Laine L, Roicin A, Shapiro D, Burgos-Vargas R, Day R, Bosi M, Ilawkey CJ, Ilochberg MC, Kvien TK, Schnitzer TJ (2000) New Eng J Med 343:1520–1528

    Article  CAS  Google Scholar 

  6. Clemett D, Goa KL (2000) Drugs 59:957–980

    Article  CAS  Google Scholar 

  7. Cannon GW (1999) Drugs Today 35:487–496

    CAS  Google Scholar 

  8. Saha NR, Sajeev C, Jadhav PR, Patil SP, Srinivasan N (2002) J Pharm Biomed Anal 28:741–751

    Article  CAS  Google Scholar 

  9. Radhakrishna OT, Rao DS, Reddy GO (2001) J Pharm Biomed Anal 59:617

    Article  Google Scholar 

  10. Damani P, Bearzotti M, Cabezon A (2003) Anal Bioanal Chem 376:1141–1146

    Article  Google Scholar 

  11. Erk N, Altuntas TG (2004) Pharmazie 59:453–456

    CAS  Google Scholar 

  12. Rao NR, Meena S, Nagaraju D, Rao AR (2005) Biomed Chromatogr 19:362–368

    Article  CAS  Google Scholar 

  13. Gowik P, Julicher B, Uhlig S (1998) J Chromatogr B 716:221–232

    Article  CAS  Google Scholar 

  14. Sultan M, Stecher G, Stoeggi WM, Bakry R, Zaborski P, Huck CW, El-Kousy NM, Bonn GK (2005) Cur Med Chem 12:573–588

    CAS  Google Scholar 

  15. Sreenivasa RB, Murthy R, Prasanna SAL (1997) Eastern Pharmacist 40:47–49

    Google Scholar 

  16. Hercegova A, Polonsky J (1999) Pharmazie 54:479–486

    CAS  Google Scholar 

  17. Rao RN, Meena S, Rao AR (2005) J Pharm Biomed Anal 39:349–363

    Article  CAS  Google Scholar 

  18. Li K, Zhao FL, Yuan YS, Tan L (1995) J Liquid Chromatogr 18:2205–2216

    Article  CAS  Google Scholar 

  19. Lee HW, Won KJ, Cho SH, Ha YH, Park WS, Yim HT, Baek M, Rew JH, Yoon SH, Yim SV, Chung JH, Lee KT (2005) J Chromatogr B 821:215–220

    Article  CAS  Google Scholar 

  20. Aravind MK, Prescilla R, Ofenstein JP (2002) J Chromatogr Sci 40:26–28

    CAS  Google Scholar 

  21. Amini M, Hamedani MP, Vosooghi M, Nabavi M, Shafiee A (2005) Anal Bioanal Chem 381:1265–1268

    Article  Google Scholar 

  22. Jalalizadeh H, Amini M, Ziaee V, Safa A, Farsam H, Shafiee A (2004) J Pharm Biomed Anal 35:665–670

    Article  CAS  Google Scholar 

  23. Stomer E, Bauer S, Kirchheiner J, Brockmoller J, Roots I (2003) J Chromatogr B 783:207–212

    Article  Google Scholar 

  24. Savaser A, Oezkan Y, Oezkan C, Tas C, Oezcan S (2004) Anal Lett 37:81–97

    Article  CAS  Google Scholar 

  25. Zhang M, Moore GA, Gardiner SJ, Begg EJ (2004) J Chromatogr B 807:217–221

    Article  CAS  Google Scholar 

  26. Vintiloiu A, Mullet WM, Papp R, Ludda D, Kwong E (2005) J Chromatogr A 1082:150–157

    Article  CAS  Google Scholar 

  27. Hamama AK, Ray J, Day R, Richard O, Brien J (2005) J Chrom Sci 43:351–354

    CAS  Google Scholar 

  28. Analytical Methods Committee (1994) Analyst 119:2363

    Article  Google Scholar 

  29. Kimura K, Oishi H, Miura T, Shorro T (1987) Anal Chem 59:2331–2334

    Article  CAS  Google Scholar 

  30. Cuadros L, García-Campaña A, Linares C, Román M (1993) Anal Lett 26(6):1243–1246

    Google Scholar 

  31. Siu SSN, Yeung JHK, Lau TK (2000) Hum Reprod 15(11):2423–2425

    Article  CAS  Google Scholar 

  32. Verbruggen G, Veys EM, Malfait AM, Schatteman L, Wieme N, Nimmegeers J, Gerin MG, Broddelez C (1990) Clin Rheumatol 9:32–41

    Article  CAS  Google Scholar 

  33. Davies NM, Mclachlan AJ, Day RO, Williams KM (2000) Clin Pharmacokinet 38:225–242

    Article  Google Scholar 

Download references

Acknowledgments

We thank Pharmacia (Pfizer) for kindly supplying the standard of CEL and Merck & Co., Inc. for kindly supplying the standard ROF.

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Authors and Affiliations

  1. Solid Phase Spectrometry Research Group, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Campus Fuentenueva, 18071, Granada, Spain

    Natalia Navas, Rocio Ureña & Luis-Fermin Capitan-Vallvey

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  1. Natalia Navas
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  2. Rocio Ureña
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  3. Luis-Fermin Capitan-Vallvey
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Correspondence to Natalia Navas.

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Navas, N., Ureña, R. & Capitan-Vallvey, LF. Determination of Celecoxib, Rofecoxib, Sodium Diclofenac and Niflumic Acid in Human Serum Samples by HPLC with DAD Detection. Chroma 67, 55–61 (2008). https://doi.org/10.1365/s10337-007-0445-x

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  • DOI: https://doi.org/10.1365/s10337-007-0445-x

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