Summary
A model system was developed for determination of the most important factors affecting a polymerization reaction used to obtain polymeric beads for liquid chromatography. An initial series of experiments has established the identity and concentration of the most effective protective colloid, the relative percentages of organic solvent and porogen compounds, the volumes of the aqueous and organic media, and the nature of the latter and of the initiator compound. Preparation of polymers with different specific surfaces, pore diameters, and other characteristics, for example total porous volume, is described. The results obtained show that macroporous packings have different specific surface areas and mean pore diameters depending on the cyclohexanol-to-dodecanol ratio and on the amount of monomer (monomer (hydroxyethyl or glycidyl methacrylate)-to-comonomer (ethylene glycol dimethacrylate) ratio). The characteristics do not change on treatment with sulfuric acid to obtain the diol function from the epoxy group.
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Blondeau, D., Bigan, M. & Ferreira, A. Experimental-design study of the influence of the polymerization conditions on the characteristics of copolymers used for liquid chromatography. Chromatographia 51, 713–721 (2000). https://doi.org/10.1007/BF02505410
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DOI: https://doi.org/10.1007/BF02505410