Skip to main content
SpringerLink
Log in

Procedure for the determination of biogenic amines by derivatization with dabsyl chloride and reversed phase liquid chromatography

  • Originals
  • Column Liquid Chromatography
  • Published:
Chromatographia Aims and scope Submit manuscript

Summary

Existing procedures for the determination of biogenic amines by derivatization with dabsyl chloride and separation by RPLC differ very little from those employed in amino acid analysis. Since some difficulties attributable to the higher hydrophobicity of dabsyl-amides were detected on using these procedures, important modifications are proposed, including extraction of the reaction mixture with an organic solvent, drying of the extract and redissolving the residue in acetone before injecting. Dabsylation and chromatographic conditions were also studied. Although recovery from spiked meat extracts is very good, significant matrix effects are detected when extraction is carried out from spiked whole meat samples. These adverse effects can be overcome by means of standard addition.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Lin, J.K.; Chang, J.Y.Anal. Chem. 1975, 47, 1634.

    Article  CAS  Google Scholar 

  2. Chang, J.Y.; Knetch, R.; Braun, D.G.Biochem. J. 1981, 199, 547.

    CAS  Google Scholar 

  3. Chang, J.Y.; Knetch, R.; Braun, D.G.Biochem. J. 1982, 203, 803.

    CAS  Google Scholar 

  4. Chang, J.Y.; Knetch, R.; Braun, D.G.Methods Enzymol. 1983, 91, 41.

    Article  CAS  Google Scholar 

  5. Chang, J.Y.J. Chromatogr. 1984, 295, 193.

    Article  CAS  Google Scholar 

  6. Stocchi, V.; Cucchiarini, L.; Picoli, G.; Magnani, M.;J. Chromatogr. 1985, 349, 77.

    Article  CAS  Google Scholar 

  7. Stocchi, V.; Picoli, G.; Magnani, M.; Palma, F.; Biagiarelli, B.; Cucchiarini, L.Anal. Biochem. 1989, 178, 107.

    Article  CAS  Google Scholar 

  8. Knetch, R.; Chang, J.Y.Anal. Chem. 1986, 58, 2375.

    Article  Google Scholar 

  9. Vendrell, J.; Avilés, F.X.J. Chromatogr. 1986, 358, 401.

    Article  CAS  Google Scholar 

  10. Schneider, H.J.Chromatographia 1989, 28, 45.

    Article  CAS  Google Scholar 

  11. Jansen, E.H.J.M.; Van den Berg, R.H.; Both-Miedema, R.; Doorn, L.J. Chromatogr. 1991, 553, 123.

    Article  CAS  Google Scholar 

  12. Watanabe, A.; Semba, J.; Kurumaji, A.; Kumashiro, S.; Toru, M.J. Chromatogr. 1992, 583, 241.

    CAS  Google Scholar 

  13. Drnevich, D.; Vary, T.C.J. Chromatogr. 1993, 613, 137.

    CAS  Google Scholar 

  14. Matysik, G.Chromatographia 1996, 43, 304.

    Article  Google Scholar 

  15. Krause, I.; Bockhardt, A.; Neckermann, H.; Henle T.; Klostermeyer, H.J. Chromatogr. A 1995, 715, 67.

    Article  CAS  Google Scholar 

  16. Bockhardt, A.; Krause, I.; Klostermeyer, H.Z. Lebensm. Unters Forsch 1996, 203, 65.

    Article  CAS  Google Scholar 

  17. Romero, R.; Bagur, M.G.; Sánchez-Viñas, M.; Gazquez, D.Chromatographia 2000, 51, 404.

    Article  CAS  Google Scholar 

  18. Castillo, M.A.; Castells, R.C.J. Chromatogr. A, in press.

  19. Massart, D.L.; Vandeginste, B.G.M.; Buydens, L.M.C.; De Jong, S.; Lewi, P.J.; Smeyers-Verbeke, J. InHandbook of Chemometrics and Qualimetrics: Part A: Elsevier, Amsterdam,1997.

    Google Scholar 

  20. Caulcutt, R.; Boddy, R. InStatistics for Analytical Chemists: Chapman & Hall, London,1983.

    Google Scholar 

  21. Zorn, M.E.; Gibbons, R.D.; Sonzogoni, W.C.Anal. Chem. 1997, 69, 3069.

    Article  CAS  Google Scholar 

  22. ICH Harmonized Tripartite Guideline.Validation of Analytical Procedures: Methodology. ICH 4, November1996.

  23. Snyder, L.R.; Kirkland, J.J.; Glach, J.L. InPractical HPLC Method Development: 2nd. ed., Wiley, New York,1997, Ch. 14 and 15.

    Google Scholar 

  24. Moret, S.; Conte, L.S.,J. Chromatogr. A 1996, 729, 363.

    Article  CAS  Google Scholar 

  25. Cardone, M.J.J. Assoc. Off. Anal. Chem. 1983, 66, 1283.

    Google Scholar 

  26. Castells, R.C.; Castillo, M.A.Anal. Chim. Acta 2000, 423, 179.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. División de Química Analítica, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, 1900, La Plata, Argentina

    M. A. Castillo & R. C. Castells

  2. CIDEPINT, 52 e/121 y 122, 1900, La Plata, Agentina

    M. A. Castillo & R. C. Castells

Authors
  1. M. A. Castillo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. C. Castells
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Castillo, M.A., Castells, R.C. Procedure for the determination of biogenic amines by derivatization with dabsyl chloride and reversed phase liquid chromatography. Chromatographia 54, 711–716 (2001). https://doi.org/10.1007/BF02492488

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02492488

Key Words

Search

Navigation