Summary
A new and simple HPLC method is described for the separation and quantitative determination of the (+)-and (−)-enantiomers of lansoprazole. The analytes were extracted from serum as previously described for whole lansoprazole [K. Borner, Chromatographia 45, 450–452 (1997)]. The enantiomers were separated by chromatography on a CHIRAL-AGPR column which contained covalently bound acid α1-glycoprotein as chiral selector. In the pure drug the (−)/(+) ratio was 0.99:1.01. In serum of twelve human volunteers the concentration of the (−)-enantiomer was 3 to 5 times higher than that of the (+)-enantiomer. Both enantiomers differ remarkably in their pharmacokinetics.
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Borner, K., Borner, E. & Lode, H. Separation of lansoprazole enantiomers in human serum by HPLC. Chromatographia 47, 171–175 (1998). https://doi.org/10.1007/BF02466577
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DOI: https://doi.org/10.1007/BF02466577