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Gaschromatographische Untersuchungen biogener Amine in Form ihrer Acetyl-, Propionyl-, n-Butyryl-, iso-Butyryl- und Pivaloylderivate

Gas chromatographic investigation of biogenic amines as their acetyl, propionyl, n-butyyl, iso-butyryl, and pivaloyl derivatives

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Summary

We describe a method for derivatisation of biogenic amines of the neurohormone metabolism (catecholamines, phenylethylamines and indolalkylamines). Derivatisation employed the anhydrides of acetic, propionic, n-butyric, iso-butyric and pivalic acid. The procedure can take place in an aqueous medium and results in stable and hydrophobic compounds which are quantitatively extractable by organic solvents. The acyl derivatives of biogenic amines posses good gas chromatographic properties and can be separated and analysed less than 20 minutes. Therefore the derivatisation described proves quite useful for the analytical gas chromatography of such amines.

Zusammenfassung

Zur gaschromatographischen Bestimmung wichtiger biogener Amine aus dem Neurohormonstoffwechsel (Katecholamine, Phenyläthylamine und Indolalkylakine) wird ihre Derivatisierung mit den Anhydriden der Essigsäure, Propionsäure, n-Buttersäure, iso-Buttersäure und Pivalonsäure beschrieben. Die Derivate lassen sich im wäßrigen Medium bilden. Auf diese Weise werden sie stabilisiert und hydrophobiert, so daß sie quantitativ mit Hilfe organischer Lösungsmittel aus der wäßrigen Phase isoliert werden können. Alle Derivate zeigen gute gaschromatographische Eigenschaften mit einer Auftrennung innerhalb von weniger als 20 Minuten.

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Literatur

  1. E. Änggard undG. Sedvall, Analyt. Chem.41, 1250 (1969).

    Article  Google Scholar 

  2. E. Gelpi, E. Peraltra undJ. Segura, J. Chrom. Sci.12, 701 (1974).

    Article  CAS  Google Scholar 

  3. S. H. Koslow, F. Cattabeni undE. Costa, Sciene176, 177 (1972).

    Article  CAS  Google Scholar 

  4. B. Crathorne undM. J. Saxby, J. Chromatogr.82, 373 (1973).

    Article  CAS  Google Scholar 

  5. O. Borga undM. Carle, J. Chromatogr.68, 77 (1972).

    Article  CAS  Google Scholar 

  6. M. G. Horning, A. M. Moss, E. M. Boucher undE. C. Horning, Analyt. Letters1, 311 (1968).

    Article  CAS  Google Scholar 

  7. W. C. Butts, Anal. Biochem.46, 187 (1972).

    Article  CAS  Google Scholar 

  8. W. J. A. van den Heuvel, J. Chromatogr.36, 354 (1968).

    Article  Google Scholar 

  9. M. G. Horning, A. M. Moss undE. C. Horning, Biochem. Biophys. Acta148, 597 (1967).

    Article  CAS  Google Scholar 

  10. P. Capella undE. C. Horning, Anal. Chem.38, 316 (1966).

    Article  CAS  Google Scholar 

  11. M. Donike, J. Chrom.85, 1 (1973).

    Article  CAS  Google Scholar 

  12. M. Donike, Chromatographia7, 651 (1974).

    Article  CAS  Google Scholar 

  13. D. J. Edwards undK. Blau, Anal. Chem.45, 387 (1972).

    CAS  Google Scholar 

  14. N. Narasimhachari undP. Vouros, Anal. Biochem.45, 154 (1972).

    Article  CAS  Google Scholar 

  15. C. J. W. Brooks undE. C. Horning, Anal. Chem.36, 1540 (1964).

    Article  CAS  Google Scholar 

  16. E. C. Horning, M. G. Horming, W. J. A. van den Heuvel, K. L. Knox, B. Holmstedt undC. J. W. Brooks, Analyt. Chem.36, 1546 (1964).

    Article  CAS  Google Scholar 

  17. E. Röder undJ. Merzhäuser, Z. Anal. Chem.272, 34 (1974).

    Article  Google Scholar 

  18. C. Hiemke, G. Kauert undD. A. Kalbhen, J. Chromatogr.153, 451 (1978).

    Article  CAS  Google Scholar 

  19. G. Kauert, C. Hiemke undD. A. Kalbhen (in Vorbereitung).

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Authors and Affiliations

  1. Institut für Rechtsmedizin der Universität München, Frauenlobstr. 7a, D-8000, München 2, Federal Republic of Germany

    G. Kauert

  2. Institut für Physiologische Chemie des Universitätsklinikum Essen, Hufelandstr. 55, D-4300, Essen, Federal Republic of Germany

    C. Hiemke

  3. Pharmakologisches Institut der Universität Bonn, Reuterstr. 2b, D-5300, Bonn, Federal Republic of Germany

    D. A. Kalbhen

Authors
  1. G. Kauert
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  2. C. Hiemke
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  3. D. A. Kalbhen
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Kauert, G., Hiemke, C. & Kalbhen, D.A. Gaschromatographische Untersuchungen biogener Amine in Form ihrer Acetyl-, Propionyl-, n-Butyryl-, iso-Butyryl- und Pivaloylderivate. Chromatographia 12, 226–230 (1979). https://doi.org/10.1007/BF02411366

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  • DOI: https://doi.org/10.1007/BF02411366

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