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Column dead-time for a bonded C18 phase with pure alcohols as eluents

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Summary

Anhydrous alcohol eluents are partly sorbed on bonded octadecyl phases. The sorbed fraction can best be described as a double layer. The length of the alcohol carbon chain determines the volume of the sorbed eluent layer and, consequently, that of the bulk eluent. Thus, column dead-time depends on the molecular size of the eluent. The column dead-time in such systems can be determined via mathematical linearization of the In (capacity factor) versus carbon number function only if (a) the solute chain length is below that of the bonded alkyl chain, (b) the data are very precise, (c) at least five data points are included.

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Authors and Affiliations

  1. Bereich Strahlenchemie, Hahn-Meitner-Institut für Kernforschung Berlin, D-1000, Berlin 39, Federal Republic of Germany

    H. J. Möckel & T. Freyholdt

Authors
  1. H. J. Möckel
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  2. T. Freyholdt
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Möckel, H.J., Freyholdt, T. Column dead-time for a bonded C18 phase with pure alcohols as eluents. Chromatographia 17, 215–220 (1983). https://doi.org/10.1007/BF02290560

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  • DOI: https://doi.org/10.1007/BF02290560

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