Summary
Non-porous spherical 2μm silica gel particles have been tested as packing for hydrodynamic chromatography of macromolecules (HDC). Columns packed with these particles in 250×4.6mm i.d. columns can be operated very efficiently (e.g. a reduced plate height of about 2) when the detection cell is miniaturized. The packing is suitable for the separation of polystyrenes of Mw of 104–107 by hydrodynamic chromatography and the retention behaviour of polystyrenes agrees well with the known theoretical models. Preliminary results on the applicability of HDC for the separation of biopolymers are presented.
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Kraak, J.C., Oostervink, R., Poppe, H. et al. Hydrodynamic chromatography of macromolecules on 2 μm non-porous spherical silica gel packings. Chromatographia 27, 585–590 (1989). https://doi.org/10.1007/BF02258983
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DOI: https://doi.org/10.1007/BF02258983