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HPLC Determination of Letrozole in Plasma Using Fluorescence Detection: Application to Pharmacokinetic Studies

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Abstract

A simple, rapid and sensitive HPLC method has been developed and validated for the analysis of letrozole in human plasma. The separation was achieved on a monolithic silica column using acetonitrile–phosphate buffer. A fluorescence detector was used for the quantitation with excitation and emission wavelengths at 230 and 295 nm. The assay enables the measurement of letrozole for therapeutic drug monitoring with a minimum quantification limit (LOQ) of 0.5 ng mL−1. The method involves a simple, one-step extraction procedure with complete recovery. Calibration was linear over the concentration range 0.5–80 ng mL−1. The coefficients of variation for inter-day and intra-day assay were found to be less than 8%.

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Acknowledgment

This work was supported by Noor Research and Educational Institute.

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Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, P.O. Box 14155-6153, Tehran, Iran

    Afshin Zarghi & Alireza Shafaati

  2. Department of Pharmaceutics, School of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, Iran

    Sayed M. Foroutan

  3. Noor Research and Educational Institute, Tehran, Iran

    Arash Khoddam

Authors
  1. Afshin Zarghi
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  2. Sayed M. Foroutan
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  3. Alireza Shafaati
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  4. Arash Khoddam
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Correspondence to Afshin Zarghi.

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Zarghi, A., Foroutan, S.M., Shafaati, A. et al. HPLC Determination of Letrozole in Plasma Using Fluorescence Detection: Application to Pharmacokinetic Studies. Chroma 66, 747–750 (2007). https://doi.org/10.1365/s10337-007-0381-9

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  • DOI: https://doi.org/10.1365/s10337-007-0381-9

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