Abstract
The frequency and distribution of chiasmata and the nature of terminal “associations” was re-examined in Syrian hamster spermatocytes using the 5-bromo-2′-deoxyuridine (BrdU) antibody technique (BAT) for differential chromatid labelling. Differential chromatid substitution was achieved by BrdU incorporation at the penultimate pre-meiotic S-phase followed by one of three different staining protocols: (i) fluorescence plus Giemsa (FPG), (ii) acridine orange staining or (iii) BAT. For analysis of chiasmata frequency and localization in the diplotene/diakinesis stages the resolution of FPG and acridine orange staining was comparable to that of BAT. In metaphase II chromosomes BAT was more informative than FPG and acridine orange staining and revealed small, terminal crossover exchanges. This finding proves that many terminal associations of meiotic chromosomes actually represent chiasmata at the end of the first meiotic division. Some crossover exchanges were localized in the constitutive heterochromatin of autosomes. Using BAT we also detected crossover exchanges in close vicinity to each other. This observation is reminiscent of the fact that crossing over interference means a reduction in frequency and does not imply total inhibition.
Similar content being viewed by others
References
Allen JW (1982) SCE and meitotic crossover exchange in germ cells. In: Sandberg AA (ed) Progress and topics in cytogenetics, vol 2. Sister chromatid exchange. A.R. Liss, New York, pp 297–311
Allen JW, Gwaltney CW (1984) Sister chromatid exchanges in mammalian meiotic chromosomes: In: Tice RR, Hollaender A (eds) Sister chromatid exchanges. Part B: Genetic toxicology and human studies. Plenum Press, New York, London, pp 629–644
Ashley T (1989) Chromosome banding and patterns of synapsis and crossing over. Abstracts of 10th International Chromosome Conference Uppsala, Sweden, June 18–22, p 17
Callan HG, Perry PE (1977) Recombination in male and female meiocytes contrasted. Philos Trans R Soc London [Biol] 277:227–233
Carpenter ATC (1975) Electron microscopy of meiosis inDrosophila melanogaster females. II. The recombination nodule — a recombination-associated structure at pachytene? Proc Natl Acad Sci USA 72:3186–3189
Chandley AC (1986) A model for effective pairing and recombinatin at meiosis based on early replicating sites (R-bands) along chromosomes. Hum Genet 72:50–57
Chandley AC, Mitchell AR (1988) Hypervariable minisatellite regions are sites for crossing-over at meiosis in man. Cytogenet Cell Genet 48:152–155
Donis-Keller H, Green P, Helms P, Cartinhour S, Weiffenbach B, Stephans K (1987) A genetic linkage map of the human genome. Cell 51:319–337
Dutrillaux B, Laurent C, Couturier J, Lejeune J (1973) Coloration par l'acridine orange de chromosomes préalablement traités après le 5-bromodéoxyuridine (BUDR). CR Acad Sci [III] 276:3179–3181
Fang JS, Jagiello GM (1988) An analysis of the chromomere map and chiasmata characteristics of human diplotene spermatocytes. Cytogenet Cell Genet 47:52–57
Ferguson-Smith MA (1976) Meiosis in the human male. Chromosomes Today 5:33–41
Fontana PG, Vickery VR (1974) Heterochromatin content and chiasma distribution in the megameric chromosomes ofStethophyma gracile andS. lincatum (Orthoptera:Arcidae). Chromosoma 46:375–395
Holm PB, Rasmussen SW (1983) Human meiosis. VII. Chiasma formation in human spermatocytes. Carlsberg Res Commun 48:415–456
Imai HT, Moriwaki K (1982) A re-examination of chiasma terminalization and chiasma frequency in male mice. Chromosoma 85:439–452
Jagiello GM, Fang JS, Sung WK, Ducayen MB, Wertheim I (1986) The hibernating male golden hamster as a model for studying homologue pairing, chiasma formation and disjunction during spermatogenesis. Biol Reprod 35:1037–1043
John B (1976) Myths and mechanisms of meiosis. Chromosoma 54:295–325
John B, Claridge (1974) Chromosome variation in British population ofOncopsis (Hemiptera:Cicadellidae). Chromosoma 46:77–89
John B, King M (1985) Pseudoterminalisation, terminalisation, and non-chiasmate modes of terminal association. Chromosoma 92:89–99
Jones GH (1978) Giemsa C-banding of rye meiotic chromosomes and the nature of “terminal” chiasmata. Chromosoma 66:45–57
Jones GH (1984) The control of chiasma distribution. Symp Soc Exp Biol 38:293–320
Jones GH (1987) Chiasmata. In: Moens PB (ed) Meiosis. Academic Press, New York, pp 213–243
Jones GH, Tease C (1984) Analysis of exchanges in differentially stained meiotic chromosomes ofLocusta migratoria after BrdU-substitution and FPG staining. IV. The nature of “terminal” associations. Chromosoma 89:33–36
Kanda N, Kato H (1980) Analysis of crossing-over in mouse meiotic cells by BrdU labelling technique. Chromosoma 78:113–122
Latos-Bielenska A, Hameister H, Vogel W (1987) Detection of BrdU incorporation in mammalian chromosomes by a BrdU antibody. III. Demonstration of replication patterns in highly resolved chromosomes. Hum Genet 76:293–295
Laurie DA, Hulten MA (1985) Further studies on chiasma distribution and interference in the human male. Ann Hum Genet 49:203–214
Lavappa KS (1974) Chiasma in the XY bivalent of the Chinease hamster. Lab Anim Sci 24:817–819
Mather K (1933) The relations between chiasmata and crossingover in diploid and triploidDrosophila melanogaster. J Genet 27:243–259
Murer-Orlando M, Richer C-L (1983) Heterochromatin heterogeneity in Chinese hamster sex bivalents. Cytogenet Cell Genet 35:195–199
Perry P, Wolf S (1974) New Giemsa method for the differential staining of sister chromatids. Nature 251:156–158
Polani PE, Crolla JA, Seller MJ (1981) An experimental approach to female mammalian meiosis. Differential chromosome labelling and an analysis of chiasmata in the female mouse. In: Vogel HJ, Jagiello D (eds) Bioregulators of reproduction. Academic Press, New York, pp 59–87
Popescu NC, DiPaolo JA (1979) Heterogeneity of constitutive heterochromatin in somatic Syrian hamster chromosomes. Cytogenet Cell Genet 24:53–60
Rasmussen SW, Holm PB (1980) Mechanics of meiosis. Hereditas 93:187–216
Solari AJ (1980) Synaptonemal complexes and associated structures in microspread human spermatocytes. Chromosoma 81:315–337
Speit G, Vogel W (1986) Detection of bromodeoxyuridine incorporation in mammalian chromosomes by a bromodeoxyuridine antibody. II. Demonstration of sister chromatid exchanges. Chromosoma 94:103–106
Tease C, Jones GH (1978) Analysis of exchanges in differential stained meiotic chromosomes of Locusta migratoria after BrdU-substitution and FPG staining. I. Crossover exchanges in monochiasmate bivalents. Chromosoma 69:163–178
Vogel W, Autenrieth M, Speit G (1986) Detection of bromodeoxyuridine-incorporation in mammalian chromosomes by a bromodeoxyuridine-antibody. I. Demonstration of replication patterns. Hum Genet 72:129–132
von Wettstein D (1984) The synaptonemal complex and genetic segregation. Symp Soc Exp Biol 38:195–231
Wang HC (1976) Identification of late DNA-replicating regions in Chinese hamster chromosomes using a BrdU-Giemsa technique. Chromosoma 58:255–261
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Latos-Bielenska, A., Vogel, W. Frequency and distribution of chiasmata in Syrian hamster spermatocytes studied by the BrdU antibody technique. Chromosoma 99, 267–272 (1990). https://doi.org/10.1007/BF01731702
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01731702