Abstract
Applying two-colour fluorescencein situ hybridization (FISH) we simultaneously hybridizedRBM- andTSPY-related cosmids to Y chromosomes in prophase and to released Y chromatin in interphase nuclei of man and pygmy chimpanzee. Whereas, even on prophasic Y chromosomes, no resolution of the overlappingRBM andTSPY signal clusters could be achieved, theRBM andTSPY signals are completely separated from each other in our maximum released Y chromatin stretches in interphase nuclei. These results unequivocally lend support to the view that theRBM andTSPY families have an interspersed organization on the Y chromosomes of man and higher apes. Thus, the distribution ofRBM andTSPY signals might well go back to a common organization of these genes next to each other on an ancient Y chromosome.
Similar content being viewed by others
References
Arnemann J, Epplen JT, Cooke HJ et al. (1987) A human Y-chromosomal DNA sequence expressed in testicular tissue.Nucleic Acids Res 15: 8713–8724.
Arnemann J, Jakubiczka S, Thüring S, Schmidke J (1991) Cloning and sequence analysis of a human Y chromosome-derived, testicular cDNA, TSPY.Genomics 11: 108–114.
Chandley AC, Cooke HJ (1994) Human male fertility-Y-linked genes and spermatogenesis.Hum Mol Genet 3: 1449–1452.
Fidlerová H, Senger G, Kost M, Sanseau P, Sheer D (1994) Two simple procedures for releasing chromatin from routinely fixed cells for fluorescence in situ hybridization.Cytogenet Cell Genet 65: 203–205.
Ma K, Inglis JD, Sharkey A et al. (1993) A Y chromosome gene family with RNA binding protein homology: candidates for the azoospermia factor AZF controling human spermatogenesis.Cell 75: 1287–1295.
Oakey RJ, Tyler-Smith C (1990) Y chromosome DNA haplotyping suggests that most European and Asian men are descended from one of two males.Genomics 7: 325–330.
Reijo R, Lee T-Y, Salo P et al. (1995) Diverse spermatogenic defects in humans caused by Y chromosome deletions encompassing a novel RNA-binding protein gene.Nature Genet 10: 383–392.
Schempp W, Binkele A, Arnemann J et al (1995). Comparative mapping ofYRRM- andTSPY-related cosmids in man and hominoid apes.Chrom Res 3: 227–234.
Senger G, Jones TA, Fidlerová H et al. (1994) Released chromatin: linearized DNA for high resolution fluorescencein situ hybridization.Hum Mol Genet 3: 1275–1280.
Taylor K, Ulinowski Z, Wolfe J (1991) Contig assembly of cosmid clones from the Y chromosome.Cytogenet Cell Genet 58: 2096.
Tyler-Smith C, Taylor L, Müller U (1988) Structure of a hypervariable tandemly repeated DNA sequence on the short arm of the human Y chromosome.J Mol Biol 203: 837–848.
Tyler-Smith C, Oakey RJ, Larin Z et al. (1993) Localization of DNA sequences required for human centromere function through an analysis of rearranged Y chromosomes.Nature Genet 5: 368–375.
Zhang JS, Yang-Feng TL, Müller U et al. (1992) Molecular isolation and characterization of an expressed gene from the human Y chromosome.Hum Mol Genet 1: 717–726.
Author information
Authors and Affiliations
Additional information
accepted for publication by M. Schmid
Rights and permissions
About this article
Cite this article
Conrad, C., Hierl, T., Gläser, B. et al. High-resolution fluorescencein situ hybridization ofRBM- andTSPY-related cosmids on released Y chromatin in humans and pygmy chimpanzees. Chromosome Res 4, 201–206 (1996). https://doi.org/10.1007/BF02254960
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02254960