Summary
Three strains ofMycelium Radicis atrovirens were grown in a submerged state with aeration brought about by continual shaking of the cultures (100 ml Erlenmeyer flasks containing 20 ml of substrate; shaking speed = 50–60 cycles per minute; amplitude =15 cm). The shake cultures showed a marked acceleration in growth as compared with the corresponding stationary cultures, maximum dry weight of mycelium being attained after incubation for 7 to 10 days in the former and after 13 to 20 days, respectively, in the latter cultures. The submerged mycelium produced in the shake cultures was successfully used in experiments on respiration of the fungus.
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Wikén, T., Somm, H. Wachstumsversuche mit Myzelsuspensionen vonMycelium Radicis atrovirens in Ruhe- und Schüttelkulturen. Experientia 8, 110–111 (1952). https://doi.org/10.1007/BF02301448
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DOI: https://doi.org/10.1007/BF02301448