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Immunpräzipitation Insulin-verwandter proteine mit ziegen-anti-insulinserum

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Summary

Bovine insulin, proinsulin, and intermediate form were prepared by Sephadex gelfiltration and chromatography on DEAE-cellulose. Using a goat anti-insulin serum, the cross-reactions of proinsulin and intermediate form could be shown by immuno-precipitation. A method is described for the separation of proinsulin from insulin in agar gel. New compounds related to proinsulin or insulin were found in human insulin preparations by means of immunoelectrophoresis.

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Authors and Affiliations

  1. Zentralinstitut für Diabetes « Gerhardt Katsch », Experimenteller Bereich, 2201, Karlsburg, DDR

    M. Ziegler, K. -D. Kohnert & U. Karg

Authors
  1. M. Ziegler
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  2. K. -D. Kohnert
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  3. U. Karg
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Wir danken Frl.B. Laars und HerrnD. Klatt für ausgezeichnete technische Mitarbeit.

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Ziegler, M., Kohnert, K.D. & Karg, U. Immunpräzipitation Insulin-verwandter proteine mit ziegen-anti-insulinserum. Experientia 27, 449–451 (1971). https://doi.org/10.1007/BF02137306

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  • DOI: https://doi.org/10.1007/BF02137306

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