Summary
A method for purification of Penicillinase fromBacillus cereus is described whereby we obtain the enzyme in a very pure form and in excellent yields. Starting from a crude acetone precipitate, the purification is obtained by using successive separations with ammonium sulphate, in controlled conditions, and final ion exchange.
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Baudet, P., Hagemann, G. Purification de la pénicillinase deBacillus cereus. Experientia 10, 374–376 (1954). https://doi.org/10.1007/BF02160547
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DOI: https://doi.org/10.1007/BF02160547