Skip to main content
Log in

Protein phosphorylation in the pancreatic B-cell

  • Published:
Experientia Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Amy, C.M., and Kirshner, N., Phosphorylation of adrenal medulla cell proteins in conjunction with stimulation of catecholamine secretion. J. Neurochem.36 (1981) 847–854.

    PubMed  Google Scholar 

  2. Ashcroft, S.J.H., Glucoreceptor mechanisms and the control of insulin release and biosynthesis. Diabetologia18 (1980) 5–15.

    Article  PubMed  Google Scholar 

  3. Baker, P.F., Knight, D.E., and Niggli, V., Protein phosphorylation accompanies calcium-dependent exocytosis in ‘leaky’ bovine adrenal medullary cells. J. Physiol.332 (1982) 118P.

    Google Scholar 

  4. Best, L., and Malaisse, W.J., Phospholipids and islet function. Diabetologia25 (1983) 299–305.

    Article  PubMed  Google Scholar 

  5. Brocklehurst, K.W., and Hutton, J.C., Ca2+-dependent binding of cytosolic components to insulin-secretory granules results in Ca2+-dependent protein phosphorylation. Biochem. J.210 (1983) 533–539.

    PubMed  Google Scholar 

  6. Burke, B.E., and DeLorenzo, R.J., Ca2+ and calmodulin-dependent phosphorylation of endogenous synaptic vesicle tubulin by a vesicle bound calmodulin kinase system. J. Neurochem.38 (1982) 1205–1218.

    PubMed  Google Scholar 

  7. Burridge, K., and Phillips, J.H., Association of actin and myosin with secretory granule membranes. Nature254 (1975) 526–529.

    PubMed  Google Scholar 

  8. Chertow, B.S., and Barker, G.R., The effects of vitamin A on insulin release and glucose oxidation in isolated rat islets. Endocrinology103 (1978) 1562–1572.

    PubMed  Google Scholar 

  9. Christie, M.R., and Ashcroft, S.J.H., Cyclic AMP-dependent protein phosphorylation and insulin secretion in intact islets of Langerhans. Biochem. J.218 (1984) 87–99.

    PubMed  Google Scholar 

  10. Colca, J.R., Brooks, C.L., Landt, M., and McDaniel, M.L., Correlation of Ca2+- and calmodulin-dependent protein kinase activity with secretion of insulin from islets of Langerhans. Biochem. J.212 (1983) 819–827.

    PubMed  Google Scholar 

  11. Colca, J.R., Kotagal, N., Brooks, C.L., Lacy, P.E., Landt, M., and McDaniel, M.L., Alloxan inhibition of a Ca2+- and calmodulin-dependent protein kinase activity in pancreatic islets. J. biol. Chem.258 (1983) 7260–7263.

    PubMed  Google Scholar 

  12. Dartt, D.A., Guernins, V.J., Donowitz, M., Taylor, L., and Sharp, G.W.G., Ca2+- and calmodulin-dependent protein phosphorylation in rat lacrimal gland. Biochem. J.202 (1982) 799–802.

    PubMed  Google Scholar 

  13. DeLorenzo, R.J., Calmodulin in neurotransmitter release and synaptic function. Fedn Proc.42 (1982) 2265–2272.

    Google Scholar 

  14. DeLorenzo, R.J., Freedman, S.D., Yoke, W.B., and Meurer, S.C., Stimulation of Ca2+-dependent neurotransmitter release and presynaptic nerve terminal protein phosphorylation by calmodulin and a calmodulin-like protein isolated from synaptic vesicles. Proc. natl Acad. Sci.76 (1979) 1838–1842.

    PubMed  Google Scholar 

  15. Fox, J.E.B., and Phillips, D.R., Role of phosphorylation in mediating the association of myosin with the cytoskeletal structures of human platelets. J. biol. Chem.257 (1982) 4120–4126.

    PubMed  Google Scholar 

  16. Freedman, S.D., and Jamieson, J.D., Hormone-induced protein phosphorylation. 1. Relationship between secretogogue action and endogenous protein phosphorylation in intact cells from the exocrine pancreas and parotid. J. Cell Biol.95 (1982) 903–908.

    Article  PubMed  Google Scholar 

  17. Gagerman, E., Idahl, L.-A., Meissner, H.P., and Täljedal, I.-B., Insulin release, cGMP, cAMP, and membrane potential in acetylcholine-stimulated islets. Am. J. Physiol.235 (1978) E493-E500.

    PubMed  Google Scholar 

  18. Gagliardino, J.J., Harrison, D.E., Christie, M.R., Gagliardino, E.E., and Ashcroft, S.J.H., Evidence for the participation of calmodulin in stimulus-secretion coupling in the pancreatic β-cell. Biochem. J.192 (1980) 919–927.

    PubMed  Google Scholar 

  19. Gorelick, F.S., Cohn, J.A., Freedman, S.D., Delahunt, N.G., Gershoni, J.M., and Jamieson, J.D., Calmodulin-stimulated protein kinase activity from rat pancreas. J. Cell Biol.97 (1983) 1294–1298.

    Article  PubMed  Google Scholar 

  20. Harrison, D.E., and Ashcroft, S.J.H., Effects of Ca2+, calmodulin and cyclic AMP on the phosphorylation of endogenous proteins by homogenates of rat islets of Langerhans. Biochim. biophys. Acta714 (1982) 313–319.

    PubMed  Google Scholar 

  21. Harrison, D.E., and Ashcroft, S.J.H., Phosphorylation-dephosphorylation mechanisms and insulin secretion: Ca2+-dependent enzymes of protein and phospholipid metabolism in rat islets of Langerhans. Diabetologia23 (1982) 172–173.

    Google Scholar 

  22. Henquin, J.C., Schmeer, W., and Meissner, H.P., Forskolin, an activator of adenylate cyclase, increases Ca2+-dependent electrical activity induced by glucose in mouse pancreatic β-cells. Endocrinology112 (1983) 2218–2220.

    PubMed  Google Scholar 

  23. Howell, S.L., and Tyhurst, M., Actomyosin interactions with insulin storage granules in vitro. Biochem. J.206 (1982) 157–160.

    PubMed  Google Scholar 

  24. Hutton, J.C., Penn, E.J., Jackson, P., and Hales, C.N., Isolation and characterization of calmodulin from an insulin-secretory tumor. Biochem. J.193 (1981) 875–885.

    PubMed  Google Scholar 

  25. Imaoka, T., Lynham, J.A., and Haslam, R.J., Purification and characterization of the 47,000-dalton protein phosphorylated during degranulation of human platelets. J. biol. Chem.258 (1983) 11404–11414.

    PubMed  Google Scholar 

  26. Jahn, R., and Soling, H.-D., Phosphorylation of the same specific protein during amylase release evoked by β-adrenergic or cholinergic agonists in rat and mouse parotid glands. Proc. natl. Acad. Sci.78 (1981) 6903–6906.

    PubMed  Google Scholar 

  27. Kaibuchi, K., Takai, Y., Sawamura, M., Hoshijima, M., Fujikura, T., and Nishizuka, Y., Synergistic functions of protein phosphorylation and calcium mobilization in platelet activation. J. biol. Chem.258 (1983) 6701–6704.

    PubMed  Google Scholar 

  28. Kowluru, A., and MacDonald, M.J., Protein phosphorylation in pancreatic islets; evidence for separate Ca2+ and cAMP-enhanced phosphorylation of two 57,000 Mr proteins. Biochem. biophys. Res. Commun.118 (1984) 797–804.

    Article  PubMed  Google Scholar 

  29. Landt, M., McDaniel, M.L., Bry, C.G., Kotagal, N., Colca, J.R., Lacy, P.E., and McDonald, J.M., Calmodulin-activated protein kinase activity in rat pancreatic islet cell membranes. Archs. Biochem. Biophys.213 (1982) 148–154.

    Article  Google Scholar 

  30. Levitan, I.B., Lemos, J.R., and Novak-Hofer, I., Protein phosphorylation and the regulation of ion channels. Trends Neurosci.6 (1983) 496–499.

    Article  Google Scholar 

  31. Lord, J.M., and Ashcroft, S.J.H., Identification and characterization of Ca2+-phospholipid-dependent protein kinase in rat islets and hamster β-cells. Biochem. J.219 (1984) 547–551.

    PubMed  Google Scholar 

  32. MacDonald, M.J., and Kowluru, A., Calcium-calmodulin-dependent myosin phosphorylation by pancreatic islets. Diabetes31 (1982) 566–570.

    PubMed  Google Scholar 

  33. MacDonald, M.J., Kowluru, A., Chang, C.-M., and Mertz, R.J., Calcium-dependent phosphorylation of endogenous pancreatic islet proteins, in: Biochemistry of metabolic processes, pp. 165–178. Eds D.L.F. Lennon, F.N. Stratman and R.N. Zahlten. Elsevier Science Publishing, Amsterdam 1983.

    Google Scholar 

  34. Malaisse, W.J., Lebrun, P., Herchuelz, A., Sener A., and Malaisse-Lagae, F., Synergistic effect of a tumor-promoting phorbol ester and a hypoglycemic sulfonylurea upon insulin release. Endocrinology113 (1983) 1870–1877.

    Google Scholar 

  35. Montague, W., and Howell, S.L., The mode of action of adenosine 3′∶5′-cyclic monophosphate in mammalian islets of Langerhans. Preparation and properties of islet-cell protein phosphokinase. Biochem. J.129 (1972) 551–560.

    PubMed  Google Scholar 

  36. Montague, W., and Howell, S.L., The mode of action of adenosine 3′∶5′-cyclic monophosphate in mammalian islets of Langerhans. Effects of insulin secretagogues on islet-cell protein kinase activity. Biochem. J.134 (1973) 321–327.

    PubMed  Google Scholar 

  37. Nestler, E.J., and Greengard, P., Nerve impulses increase the phosphorylation state of protein I in rabbit superior cervical ganglion. Nature296 (1982) 452–454.

    Article  PubMed  Google Scholar 

  38. Nishizuka, Y., Calcium, phospholipid turnover and transmembrane signalling. Phil. Trans. R. Soc. Lond.B302 (1983) 101–112.

    Google Scholar 

  39. Nishizuka, Y., The role of protein kinase C in cell surface signal transduction and tumor promotion. Nature308 (1984) 693–698.

    Article  Google Scholar 

  40. Ostlund, R.E., Leung, J.T., and Kipnis, D.M., Myosins of secretory tissues. J. Cell Biol.77 (1978) 827–836.

    Article  PubMed  Google Scholar 

  41. Penn, E.J., Brocklehurst, K.W., Sopwith, A.M., Hales, C.N., and Hutton, J.C., Ca2+-calmodulin dependent myosin light-chain phosphorylating activity in insulin secreting tissues. FEBS Lett.130 (1982) 4–8.

    Article  Google Scholar 

  42. Plewe, G., Jahn, R., Immelmann, A., Bode, C., and Soling, H.-D., Specific phosphorylation of a protein in calcium accumulating endoplasmic reticulum from rat parotid glands following stimulation by agonists involving cAMP as second messenger. FEBS Lett.166 (1984) 96–103.

    Article  PubMed  Google Scholar 

  43. Roberts, M.L., and Butcher, F.R., The involvement of protein phosphorylation in stimulus-secretion coupling in the mouse exocrine pancreas. Biochem. J.210 (1983) 353–359.

    PubMed  Google Scholar 

  44. Schatzman, R.C., Wise, B.C., and Kuo, J.F., Phospholipid-sensitive calcium-dependent protein kinase: inhibition by antipsychotic drugs. Biochem. biophys. Res. Commun.98 (1981) 669–676.

    Article  PubMed  Google Scholar 

  45. Schatzman, R.C., Raynor, R.L., and Kuo, J.F., N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7), a calmodulin antagonist, also inhibits phospholipid-sensitive calcium-dependent protein kinase. Biochim. biophys. Acta755 (1983) 144–147.

    PubMed  Google Scholar 

  46. Schubart, U.K., Regulation of protein phosphorylation in hamster insulinoma cells. Identification of Ca2+-regulated cytoskeletal and cAMP-regulated cytosolic phosphoproteins by two-dimensional electrophoresis. J. biol. Chem.257 (1982) 12231–12238.

    PubMed  Google Scholar 

  47. Schubart, U.K., Erlichman, J., and Fleischer, N., The role of calmodulin in the regulation of protein phosphorylation and insulin release in hamster insulinoma cells. J. biol. Chem.255 (1980) 4120–4124.

    PubMed  Google Scholar 

  48. Schubart, U.K., Erlichman, J., and Fleischer, N., Regulation of insulin release and protein phosphorylation by calcium and cyclic AMP: possible role for calmodulin, in: Calcium and Cell Function, vol. 3, pp. 381–401. Ed. W.Y. Cheung. Academic Press, 1982.

  49. Schubart, U.K., Fleischer, N., and Erlichman, J., Ca2+-dependent protein phosphorylation and insulin release in intact hamster insulinoma cells. Inhibition by trifluoperazine. J. biol. Chem.255 (1980) 11063–11066.

    PubMed  Google Scholar 

  50. Schubart, U.K., Scharpiro, S., Fleischer, N., and Rosen, O.M., Cyclic adenosine 3′∶5′-monophosphate-mediated insulin secretion and ribosomal protein phosphorylation in a hamster islet cells tumor. J. biol. Chem.252 (1977) 92–101.

    PubMed  Google Scholar 

  51. Sharp, G.W.G., The adenylate cyclase-cyclic AMP system in islets of Langerhans and its role in the control of insulin release. Diabetologia16 (1979) 287–296.

    Article  PubMed  Google Scholar 

  52. Shaw, J.O., and Lyons, R.M., Requirement for different Ca2+ pools in the activation of rabbit platelets. 1. Release reaction and protein phosphorylation. Biochim. biophys. Acta714 (1982) 492–499.

    PubMed  Google Scholar 

  53. Sieghart, W., Theoharides, T.C., Alper, S.L., Douglas, W.W., and Greengard, P., Calcium-dependent protein phosphorylation during secretion by exocytosis in the mast cell. Nature275 (1978) 329–331.

    PubMed  Google Scholar 

  54. Spruill, W.A., Zysk, J.R., Tres, L.L., and Kierszenbaum, A.L., Calcium/calmodulin-dependent phosphorylation of vimentin in rat Sertoli cells. Proc. natl. Acad. Sci.80, (1983) 760–764.

    PubMed  Google Scholar 

  55. Sugden, M.C., Ashcroft, S.J.H., and Sugden, P.H., Protein kinase activities in rat pancreatic islets of Langerhans. Biochem. J.180 (1979) 219–229.

    PubMed  Google Scholar 

  56. Sugden, M.C., Christie, M.R., and Ashcroft, S.J.H., Presence and possible role of calcium-dependent regulator (calmodulin) in rat islets of Langerhans. FEBS Lett.105 (1979) 95–100.

    Article  PubMed  Google Scholar 

  57. Suzuki, S., Oka, H., Yasuda, H., Ikeda, M., Cheng, P.Y., and Oda, T., Effect of glucose on protein phosphorylation in rat pancreatic islets. Biochem. biophys. Res. Commun.99 (1981) 987–993.

    Article  PubMed  Google Scholar 

  58. Suzuki, S., Oka, H., Yasuda, H., Ikeda, M., Cheng, P.Y., and Oda T., Effect of glucagon and cyclic adenosine 3′∶5′-monophosphate on protein phosphorylation in rat pancreatic islets. Endocrinology112 (1983) 348–352.

    PubMed  Google Scholar 

  59. Tanigawa, K., Kuzuya, H., Imura, H., Taniguchi, H., Baba, S., Takai, Y., and Nishizuka, Y., Calcium-activated, phospholipid-dependent protein kinase in rat pancreas islets of Langerhans. FEBS Lett.138 (1982) 183–186.

    Article  PubMed  Google Scholar 

  60. Walsh, D.A., and Cooper, R.H., The physiological regulation and function of cAMP-dependent protein kinases. Biochem. Action Hormones6 (1979) 1–75.

    Google Scholar 

  61. Zawalich, W., Brown, C., and Rasmussen, H., Insulin secretion: combined effects of phorbol ester and A23187. Biochem. biophys. Res. Commun.117 (1983) 448–455.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Harrison, D.E., Ashcroft, S.J.H., Christie, M.R. et al. Protein phosphorylation in the pancreatic B-cell. Experientia 40, 1075–1084 (1984). https://doi.org/10.1007/BF01971454

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01971454

Key words

Navigation