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The effect of pyrophosphate and diphosphonates on calcium transport in red cells

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  • Chimica, Biochimica
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Summary

The effect of 0.5 mM pyrophosphate (PPi), disodium ethane-1-hydroxy-1, 1-diphosphonate (EHDP) and disodium dichloromethane diphosphonate (Cl2MDP) on the ATP-dependent Ca2+ extrusion from the human red cell ghosts was studied. PPi and Cl2MDP had no effect, when introduced into the cells or added outside to the medium. EHDP slightly increased the calcium concentration in the released cells and slightly decreased the rate constant of the calcium transport, having opposite effects when it was inside or outside the cells. PPi and the 2 diphosphonates were not found to move easily across the red cell membrane.

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Abbreviations

PPi:

pyrophosphate

EHDP:

disodium ethane-1-hydroxy-1, 1-diphosphonate

Cl2MDP:

disodium dichloromethane diphosphonate

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Authors and Affiliations

  1. Department of Pathophysiology, University of Berne, Murtenstrasse 35, CH-3010, Berne, Switzerland

    R. Felix & H. Fleisch

Authors
  1. R. Felix
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  2. H. Fleisch
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Additional information

Acknowledgments. We thank DrH. J. Schatzmann for introducing us to the methods of calcium transport out of human red cells. We are very grateful to Miss M. Leu for skilled assistance and Mrs B. Gyger for preparing the manuscript.—This work has been supported by the Swiss National Science Foundation (3.725/76) and by the Procter & Gamble Company, Cincinnati, USA.

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Felix, R., Fleisch, H. The effect of pyrophosphate and diphosphonates on calcium transport in red cells. Experientia 33, 1003–1005 (1977). https://doi.org/10.1007/BF01945933

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