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Isolation of plasmid DNA fromAgrobacterium by isopycnic density gradient centrifugation in vertical rotors

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Summary

Fixed angle and vertical rotors are compared in terms of their sample load, run time, and resolution for the preparation of plasmid DNA fromAgrobacterium.

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References

  1. M.R. Davey, E.C. Cocking, J.P. Freeman, N. Pearce and I. Tudor, Pl. Sci. Lett.18, 307 (1980).

    Article  CAS  Google Scholar 

  2. J.P. Hernalsteens, F. Van Vliet, M. DeBeuckeleer, A. Depicker, G. Engler, H. Lemmers, M. Holsters, M. Van Montagu and J. Schell, Nature287, 684 (1980).

    Article  Google Scholar 

  3. M.-D. Chilton, R.K. Saiki, N. Yadov, M.P. Gordon and F. Quetier, Proc. natl Acad. Sci. USA77, 4060 (1980).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. I. Zaenen, N. Van Larabecke, H.J. Teuchy, M. Van Montagu and J. Schell, J. molec. Biol.86, 109 (1974).

    Article  CAS  PubMed  Google Scholar 

  5. M.R. Davey, J.P. Freeman, J. Draper and E.C. Cocking, in: Methods in Plant Pathology, p. 209. Ed. D.S. Ingram and J.P. Helgeson. Blackwell Scientific Publications Ltd, London 1980.

    Google Scholar 

  6. G.M. Richards, Analyt. Biochem.57, 369 (1974).

    Article  CAS  PubMed  Google Scholar 

  7. T.C. Currier and E.W. Nester, Analyt. Biochem.76, 431 (1976).

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Plant Genetic Manipulation Group, Department of Botany, University of Nottingham, University Park, NG7 2RD, Nottingham, England

    J. Draper, M. R. Davey, J. P. Freeman & E. C. Cocking

Authors
  1. J. Draper
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  2. M. R. Davey
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  3. J. P. Freeman
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  4. E. C. Cocking
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Additional information

Acknowledgments. This work was supported by a CASE Studentship from the SRC and ICI Corporate Laboratories to J.D., and a grant from the SRC to M.R.D. and E.C.C. The authors thank Mr B.V. Case for photographic assistance.

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Draper, J., Davey, M.R., Freeman, J.P. et al. Isolation of plasmid DNA fromAgrobacterium by isopycnic density gradient centrifugation in vertical rotors. Experientia 38, 101–102 (1982). https://doi.org/10.1007/BF01944551

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  • DOI: https://doi.org/10.1007/BF01944551

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