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Visualization of DNA in various phages (T4, χ, T7, ϕ 29) by ethidium bromide epi-fluorescent microscopy

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Summary

DNA-containing areas in various phages (T4, χ, T7 and ϕ29) could be observed at the light microscopic level using ethidium bromide epi-fluorescent microscopy. The fluorescent intensity per phage was in linear proportion to the DNA content in each phage.

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Authors and Affiliations

  1. Department of Cell Biology, National Institute for Basic Biology, 444, Okazaki, Japan

    T. Kuroiwa, S. Nishibayashi, S. Kawano & T. Suzuki

Authors
  1. T. Kuroiwa
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  2. S. Nishibayashi
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  3. S. Kawano
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  4. T. Suzuki
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Additional information

Acknowledgements. This work was supported in part by the grant No. 521708 and No. 511212 from the Japan Ministry of Education, Science and Culture.

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Kuroiwa, T., Nishibayashi, S., Kawano, S. et al. Visualization of DNA in various phages (T4, χ, T7, ϕ 29) by ethidium bromide epi-fluorescent microscopy. Experientia 37, 969–971 (1981). https://doi.org/10.1007/BF01971784

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  • DOI: https://doi.org/10.1007/BF01971784

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